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Int J Implant Dent:不同大小的钛和氧化锆颗粒对细胞的影响

2019-07-30 sunshine 译 MedSci原创

本研究旨在评估不同大小的钛(Ti)和氧化锆(Zr)颗粒对(1)骨肉瘤来源的成骨细胞(SaOs-2)和人牙龈成纤维细胞(HGF)的代谢活性和(2)单核细胞的细胞因子(THP-1)表达的影响。将Ti(60-80nm和100nm)和Zr(2μm和75μm)颗粒与SaOs-2,HGF和THP-1细胞一起温育。在第0,2,4和7天以及0,1,2和4(THP-1),评估线粒体活性,并使用酶联免疫吸附测定法测定

本研究旨在评估不同大小的钛(Ti)和氧化锆(Zr)颗粒对(1)骨肉瘤来源的成骨细胞(SaOs-2)和人牙龈成纤维细胞(HGF)的代谢活性和(2)单核细胞的细胞因子(THP-1)表达的影响。

将Ti(60-80nm和100nm)和Zr(2μm和75μm)颗粒与SaOs-2,HGF和THP-1细胞一起温育。在第0,2,4和7天以及0,1,2和4(THP-1),评估线粒体活性,并使用酶联免疫吸附测定法测定第1天刺激THP-1的白细胞介素(IL)-1β和IL- 6的浓度。

结果显示,Ti60-80,Ti100,Zr2和Zr75颗粒与SaOs-2和HGF细胞活力的逐渐及明显降低相关。但是这些影响在Zr组中不太明显。与对照细胞相似,THP-1未显示IL-1β和IL-6浓度的增加。在Ti100存在下,THP-1的活力仅受损。

综上所述,该研究结果表明,Ti和Zr颗粒对SaOs-2和HGF的活力具有不利影响,但对THP-1没有促炎作用。

原始出处:

Schwarz F, Langer M, et al., Cytotoxicity and proinflammatory effects of titanium and zirconia particles. Int J Implant Dent. 2019 Jul 9;5(1):25. doi: 10.1186/s40729-019-0178-2.

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    2020-01-20 xuyong536
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    2020-05-12 30397613
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    2020-01-06 xuyu
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    2019-07-30 医者仁心5538

    学习了

    0

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