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CELL: 人类蛋白质组首次完整定量检测

2016-08-08 佚名 生物谷

尽管2003年,人们成功地了完成人类基因组计划(Human Genome Project),构建出所有人类基因的目录,但是大多数蛋白质研究仍然聚焦在绘制出人类基因组图谱之前科学家们研究的蛋白中相对较小的一部分蛋白上。若要超越这种停滞不前的蛋白质-基因组学研究方法,就应需要为几乎每种人类蛋白开发高度特异性的检测方法。2016年7月28日的Cell期刊上,来自美国系统生物学研究所的Ulrike K

尽管2003年,人们成功地了完成人类基因组计划(Human Genome Project),构建出所有人类基因的目录,但是大多数蛋白质研究仍然聚焦在绘制出人类基因组图谱之前科学家们研究的蛋白中相对较小的一部分蛋白上。若要超越这种停滞不前的蛋白质-基因组学研究方法,就应需要为几乎每种人类蛋白开发高度特异性的检测方法。

2016年7月28日的Cell期刊上,来自美国系统生物学研究所的Ulrike Kusebauch博士、Robert Moritz教授和来自瑞士苏黎世联邦理工学院的Ruedi Aebersold发表的论文标题为“Human SRMAtlas: A Resource of Targeted Assays to Quantify the Complete Human Proteome”的文章,在该文中,研究人员利用166174种已被充分了解的化学合成蛋白特征性肽(proteotypic peptide)开发出一种被称作选择性反应监控(selected reaction monitoring,SRM)的技术。利用该技术可以靶向识别和可重复地定量预测人类蛋白质组中所有蛋白质的高度特异性质谱检测方法及其汇编目录,包括许多剪接变异体、非同义突变和翻译后修饰。

SRMAtlas资源可以在http://www.srmatlas.org网站上免费获取。研究人员期待这一资源将极大地加快基于蛋白质的实验室生物学发展从而有助理解疾病转化和健康轨迹,这是因为如今在理论上能够鉴定和定量检测出任何样品中的任何人类蛋白。

个人化医学将依赖于分子特征来监控人们的健康状态,提供信号来鉴定健康轨迹发生的变化,以及首先在临床试验随后在临床实践中提供信息来让合适的患者匹配正确的药物。这种人类SRMAtlas计划稳步地将蛋白组学推到前沿,并且为蛋白质组学在癌症登月计划(Cancer Moonshot)中发挥较大的作用提供了可能。

原始出处

Kusebauch U, Campbell DS, Deutsch EW, Chu CS, Spicer DA, Brusniak MY, Slagel J, Sun Z, Stevens J, Grimes B, Shteynberg D, Hoopmann MR, Blattmann P, Ratushny AV, Rinner O, Picotti P, Carapito C, Huang CY, Kapousouz M, Lam H, Tran T, Demir E, Aitchison JD, Sander C,Hood L, Aebersold R, Moritz RL.Human SRMAtlas: A Resource of Targeted Assays to Quantify the Complete Human Proteome.Cell. 2016 Jul 28

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    2016-10-22 维他命
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