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Tissue Eng Part A：水凝胶硬度对人脂肪干细胞微球的分化的影响

2019-01-13 MedSci MedSci原创

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水凝胶代表了用于实现三维（3D）组织工程（TE）构建体的有吸引力的材料平台，因为它们具有可调节的机械性质，与不同类型的细胞相容并且类似于天然细胞外基质中发现的元素。到目前为止，通过利用单细胞包封方法进行了许多水凝胶-软骨/骨TE相关研究。尽管多细胞球体培养物对软骨或骨TE表现出有利的性质，但水凝胶内干细胞微球的软骨形成或成骨分化潜力尚未得到很多研究。本研究首次探讨了明胶基水凝胶（储能模量为538,

水凝胶代表了用于实现三维（3D）组织工程（TE）构建体的有吸引力的材料平台，因为它们具有可调节的机械性质，与不同类型的细胞相容并且类似于天然细胞外基质中发现的元素。到目前为止，通过利用单细胞包封方法进行了许多水凝胶-软骨/骨TE相关研究。尽管多细胞球体培养物对软骨或骨TE表现出有利的性质，但水凝胶内干细胞微球的软骨形成或成骨分化潜力尚未得到很多研究。

本研究首次探讨了明胶基水凝胶（储能模量为538,3584或7263 Pa）的刚度如何影响由端粒酶永生化人脂肪干细胞形成的微球的增殖和分化（hASC /端粒酶逆转录酶）。共聚焦显微镜表明，所有测试的水凝胶在对照，软骨形成或成骨培养基中的3-5周培养期间支持细胞活力。虽然在较软的水凝胶中，来自相邻微球的细胞在几天内开始长出并相互连接，但它们的突出分别在较硬的水凝胶或在软骨形成培养基中培养的较慢或受限。在所有测试的水凝胶中检测到的软骨形成标志物（SOX9，ACAN，COL2A1）的高表达证明，hASC/hTERT微球的软骨形成分化非常成功，特别是在两种较软的水凝胶中，其中阿尔新安证实了软骨特异性质。这些软骨诱导的样品也表达COL10A1，它是软骨细胞肥大的标志物。有趣的是，水凝胶本身（没有分化培养基）显示出轻微的软骨诱导。无论水凝胶硬度如何，在用成骨培养基刺激的样品中，所选标记物RUNX2，BGLAP，ALPL和COL1A1的表达不是决定性的。尽管如此，von Kossa染色证实两种较软水凝胶中的骨生成刺激样品中存在钙沉积，这表明这些也有利于骨生成。茜素红定量测定也证实了这一观察结果，在成骨诱导水凝胶中检测到的钙含量高于对照组。

综上所述，这些结果表明，脂肪来源的干细胞微球体在明胶基水凝胶中的包封显示出未来在软骨或骨TE中应用的潜力。

原始出处：

Zigon-Branc S, Markovic M, et al., Impact of hydrogel stiffness on differentiation of human adipose-derived stem cell microspheroids. Tissue Eng Part A. 2019 Jan 11. doi: 10.1089/ten.TEA.2018.0237.

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    2019-12-14 spoonycyy

    #Eng#

    0

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    2019-09-21 xuyu

    #Tissue#

    0

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    2019-01-14 tastas

    #水凝胶#

    0

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    2019-01-14 zhaojie88

    #ART#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=2081522, encodeId=5086208152214, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Sat Dec 14 19:27:00 CST 2019, time=2019-12-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911371, encodeId=bbaf19113e1be, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Sat Sep 21 22:27:00 CST 2019, time=2019-09-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308035, encodeId=70b11308035f0, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Mon Jan 14 17:27:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357078, encodeId=5d84135e078e7, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon Jan 14 17:27:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1439003, encodeId=309514390039b, content=<a href='/topic/show?id=dd2884346fe' target=_blank style='color:#2F92EE;'>#脂肪干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=84346, encryptionId=dd2884346fe, topicName=脂肪干细胞)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Mon Jan 14 17:27:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1460996, encodeId=83f71460996b7, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Mon Jan 14 17:27:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=357994, encodeId=e39f35e994ee, content=好文，值得点赞！认真学习，应用于实践！谢谢分享给广大同好！, beContent=null, objectType=article, channel=null, level=null, likeNumber=109, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRuUXgacXpPeW0x8Z3Qr9c3I2kTic4ZIpR29iaftYgkjRcbv982icYMtqANBPYBgPnOia6mVtX76qMXuc/0, createdBy=d85d1636106, createdName=lietome2, createdTime=Sun Jan 13 08:44:14 CST 2019, time=2019-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=357981, encodeId=155b35e981be, content=学习学习再学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Sun Jan 13 05:12:17 CST 2019, time=2019-01-13, status=1, ipAttribution=)]
    2019-01-14 zhouqu_8

    #脂肪干细胞#

    0

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    2019-01-14 lishizhe

    #凝胶#

    0

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    2019-01-13 lietome2

    好文，值得点赞！认真学习，应用于实践！谢谢分享给广大同好！

    0

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    2019-01-13 yjs木玉

    学习学习再学习

    0

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