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使用BMP-2在大鼠腓骨缺损模型中验证骨再生的发生

2018-01-21 MedSci MedSci原创

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本研究旨在评估三个骨形态发生蛋白（BMP）及其载体在大鼠腓骨临界尺寸节段性缺损（CSD）模型中的修复作用，并探究动物模型和载体的研发以更好的用于骨再生。在实验1、2和3中分别使用14、16和24只双侧腓骨上存在CSDs的大鼠。使用具有可吸收胶原海绵（ACS）（实验1和2）、高压灭菌自体骨（AAB）和纤维蛋白胶（FG）（实验3）以及异种骨（实验2）的BMP-2进行实验。在每个实验的随访期间进行X线和

本研究旨在评估三个骨形态发生蛋白（BMP）及其载体在大鼠腓骨临界尺寸节段性缺损（CSD）模型中的修复作用，并探究动物模型和载体的研发以更好的用于骨再生。

在实验1、2和3中分别使用14、16和24只双侧腓骨上存在CSDs的大鼠。使用具有可吸收胶原海绵（ACS）（实验1和2）、高压灭菌自体骨（AAB）和纤维蛋白胶（FG）（实验3）以及异种骨（实验2）的BMP-2进行实验。在每个实验的随访期间进行X线和组织形态学评估。

结果显示，与对照和异种移植（猪骨）组相比，所有使用BMP-2的实验组中均观察到明显的新骨形成。虽然基于BMP载体的实验结果存在一些差异，但是在最初形成过多的骨之后，通过重塑明显减少了再生骨量。

综上所述，该研究结果表明，BMP-2由于其骨诱导性而表现出优异的骨再生能力，但是其递送系统不同效果也明显不同。ACS和FG表现出较好的骨再生能力，满足BMP载体靶向和控释的必要条件。AAB不能提供作为BMP载体的控释，但其维持空间的作用较为明显。能够为BMP/载体复合物提供足够支持的载体和支架对于大的骨缺陷是必需的，且AAB可作为有效的骨缺损修复支架来加以应用。大鼠腓骨CSD模型简单有效，可用于BMPs等药物对骨再生的初步评估。

原始出处：

Jung-Woo Nam, Hyung-Jun Kim. Stepwise verification of bone regeneration using recombinant human bone morphogenetic protein-2 in rat fibula model. J Korean Assoc Oral Maxillofac Surg. 2017 Dec; 43(6): 373–387.  doi:  10.5125/jkaoms.2017.43.6.373

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    2018-11-03 12498f17m73暂无昵称

    #BMP-2#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1642646, encodeId=b9741642646c5, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Sat Nov 03 09:04:00 CST 2018, time=2018-11-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634018, encodeId=c057163401876, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Fri Nov 30 14:04:00 CST 2018, time=2018-11-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1419249, encodeId=90ca141924955, content=<a href='/topic/show?id=2e2e10182602' target=_blank style='color:#2F92EE;'>#骨缺损#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101826, encryptionId=2e2e10182602, topicName=骨缺损)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=51173492599, createdName=lfcmxl, createdTime=Tue Jan 23 05:04:00 CST 2018, time=2018-01-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472097, encodeId=2c2f14e2097c0, content=<a href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Tue Jan 23 05:04:00 CST 2018, time=2018-01-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=280718, encodeId=d76a280e1875, content=学习..谢谢分享., beContent=null, objectType=article, channel=null, level=null, likeNumber=100, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/xkvRjYnJzzRJ2R0y7VhicibvkjPWHjIMmu4ZpKnE9cWAu4lVicS3uDUCfF4M4JWvrkrYfpb2KehPV0Ee29tPqyPEebBUibiaKhnvD/0, createdBy=f8412001184, createdName=惠映实验室, createdTime=Sun Jan 21 15:10:48 CST 2018, time=2018-01-21, status=1, ipAttribution=)]
    2018-11-30 HNYYM

    #BMP#

    0

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    2018-01-23 lfcmxl

    #骨缺损#

    0

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    2018-01-23 lsj630

    #骨再生#

    0

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    2018-01-21 惠映实验室

    学习..谢谢分享.

    0

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