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Sci Rep：载入地塞米松纳米球的RUNX2/SP7和ATF4的顺序转染增强了骨生成

2019-02-05 MedSci MedSci原创

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基因转染的时间对干细胞分化有很大影响。顺序转染对细胞行为的调节至关重要。当分化开始后几天转染时，在分化晚期表达的基因可以调节细胞行为和功能。为了确定关键基因递送的最佳时间，我们依次转染人间充质干细胞（hMSCs）。该方法可以容易地控制干细胞的成骨。首先用RUNX2和SP7转染hMSCs，使用聚（乳酸-共-羟基乙酸）纳米颗粒诱导成骨，然后在5,7和14天后用ATF4转染。在用所有三种基因转染hMSC

基因转染的时间对干细胞分化有很大影响。顺序转染对细胞行为的调节至关重要。当分化开始后几天转染时，在分化晚期表达的基因可以调节细胞行为和功能。为了确定关键基因递送的最佳时间，我们依次转染人间充质干细胞（hMSCs）。该方法可以容易地控制干细胞的成骨。

首先用RUNX2和SP7转染hMSCs，使用聚（乳酸-共-羟基乙酸）纳米颗粒诱导成骨，然后在5,7和14天后用ATF4转染。在用所有三种基因转染hMSC之前，单独转染每种基因并监测其表达。通过RT-PCR，Western印迹和共聚焦显微镜确认这些基因的转染。pDNA进入hMSCs的细胞核，RUNX2和SP7蛋白被翻译并触发成骨。其次，当细胞处于成骨细胞前阶段时，ATF4基因被递送。为了诱导hMSCs的成骨，在RUNX2/SP7转染后14天ATF4基因递送的最佳时间。

结果显示，在2维和3维培养系统中，RUNX2/SP7后14天转染ATF4促进了hMSCs的成骨分化。

原始出处：

Hye Jin Kim, Ji Sun Park, et al., Sequential transfection of RUNX2/SP7 and ATF4 coated onto dexamethasone-loaded nanospheresenhances osteogenesis. Sci Rep. 2018; 8: 1447.

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    2019-02-26 stfoxst

    #Runx2#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1669011, encodeId=ed381669011e5, content=<a href='/topic/show?id=f4c915e4262' target=_blank style='color:#2F92EE;'>#Runx2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15742, encryptionId=f4c915e4262, topicName=Runx2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=635226492904, createdName=stfoxst, createdTime=Tue Feb 26 00:28:00 CST 2019, time=2019-02-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1804570, encodeId=eb7118045e09b, content=<a href='/topic/show?id=97bee74149c' target=_blank style='color:#2F92EE;'>#纳米球#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77414, encryptionId=97bee74149c, topicName=纳米球)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1c8c241, createdName=w363522449, createdTime=Wed Dec 11 23:28:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360170, encodeId=fa1a3601e077, content=学习了谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=104, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160304/IMG56D94856B1B5D6405.jpg, createdBy=27931687771, createdName=一个字-牛, createdTime=Fri Feb 08 08:45:07 CST 2019, time=2019-02-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360093, encodeId=315336009320, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Thu Feb 07 08:09:37 CST 2019, time=2019-02-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557956, encodeId=2502155e95662, content=<a href='/topic/show?id=6dcc2935bd' target=_blank style='color:#2F92EE;'>#ATF4#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2935, encryptionId=6dcc2935bd, topicName=ATF4)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7df414933799, createdName=appleandpeer, createdTime=Wed Feb 06 16:28:00 CST 2019, time=2019-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=359968, encodeId=a84f35996865, content=好, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Tue Feb 05 07:10:51 CST 2019, time=2019-02-05, status=1, ipAttribution=)]
    2019-12-11 w363522449

    #纳米球#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1669011, encodeId=ed381669011e5, content=<a href='/topic/show?id=f4c915e4262' target=_blank style='color:#2F92EE;'>#Runx2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15742, encryptionId=f4c915e4262, topicName=Runx2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=635226492904, createdName=stfoxst, createdTime=Tue Feb 26 00:28:00 CST 2019, time=2019-02-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1804570, encodeId=eb7118045e09b, content=<a href='/topic/show?id=97bee74149c' target=_blank style='color:#2F92EE;'>#纳米球#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77414, encryptionId=97bee74149c, topicName=纳米球)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1c8c241, createdName=w363522449, createdTime=Wed Dec 11 23:28:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360170, encodeId=fa1a3601e077, content=学习了谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=104, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160304/IMG56D94856B1B5D6405.jpg, createdBy=27931687771, createdName=一个字-牛, createdTime=Fri Feb 08 08:45:07 CST 2019, time=2019-02-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360093, encodeId=315336009320, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Thu Feb 07 08:09:37 CST 2019, time=2019-02-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557956, encodeId=2502155e95662, content=<a href='/topic/show?id=6dcc2935bd' target=_blank style='color:#2F92EE;'>#ATF4#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2935, encryptionId=6dcc2935bd, topicName=ATF4)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7df414933799, createdName=appleandpeer, createdTime=Wed Feb 06 16:28:00 CST 2019, time=2019-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=359968, encodeId=a84f35996865, content=好, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Tue Feb 05 07:10:51 CST 2019, time=2019-02-05, status=1, ipAttribution=)]
    2019-02-08 一个字-牛

    学习了谢谢分享

    0

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    2019-02-07 龙胆草

    学习谢谢分享

    0

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    2019-02-06 appleandpeer

    #ATF4#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1669011, encodeId=ed381669011e5, content=<a href='/topic/show?id=f4c915e4262' target=_blank style='color:#2F92EE;'>#Runx2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15742, encryptionId=f4c915e4262, topicName=Runx2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=635226492904, createdName=stfoxst, createdTime=Tue Feb 26 00:28:00 CST 2019, time=2019-02-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1804570, encodeId=eb7118045e09b, content=<a href='/topic/show?id=97bee74149c' target=_blank style='color:#2F92EE;'>#纳米球#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77414, encryptionId=97bee74149c, topicName=纳米球)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1c8c241, createdName=w363522449, createdTime=Wed Dec 11 23:28:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360170, encodeId=fa1a3601e077, content=学习了谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=104, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160304/IMG56D94856B1B5D6405.jpg, createdBy=27931687771, createdName=一个字-牛, createdTime=Fri Feb 08 08:45:07 CST 2019, time=2019-02-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360093, encodeId=315336009320, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Thu Feb 07 08:09:37 CST 2019, time=2019-02-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557956, encodeId=2502155e95662, content=<a href='/topic/show?id=6dcc2935bd' target=_blank style='color:#2F92EE;'>#ATF4#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2935, encryptionId=6dcc2935bd, topicName=ATF4)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7df414933799, createdName=appleandpeer, createdTime=Wed Feb 06 16:28:00 CST 2019, time=2019-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=359968, encodeId=a84f35996865, content=好, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Tue Feb 05 07:10:51 CST 2019, time=2019-02-05, status=1, ipAttribution=)]
    2019-02-05 1e1c07bdm01(暂无匿称)

    好

    0

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