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Clinica Chimica Acta：柱后茚三酮衍生化液相色谱法与串联质谱法、离子交换色谱法测定氨基酸的比较

2019-09-22 gladiator MedSci原创

测定氨基酸
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氨基酸的精确定量是成功诊断和监测代谢疾病患者的必要条件。我们比较了离子交换色谱法(IEC)和液相色谱法与串联质谱法(LC-MS/MS)，这两种方法是临床实验室中最常用的测定生理样品中AAs的方法。

氨基酸的精确定量是成功诊断和监测代谢疾病患者的必要条件。我们比较了离子交换色谱法(IEC)和液相色谱法与串联质谱法(LC-MS/MS)，这两种方法是临床实验室中最常用的测定生理样品中AAs的方法。

研究人员将123名明显健康的儿童研究对象。根据AA分析仪Pinnacle PCX上皮克林实验室的协议，根据aTRAQ试剂盒将LC-MS/MS血浆样品制备成用于Sciex 3200 Qtrap上的检测样。结果采用皮尔逊相关系数和百分差布兰-阿尔特曼检验解释。

我们评估的14个AAs的Spearman相关系数，从Tau的0.67到Leu和Thr的0.89不等。11个AAs的测量均值差异(IEC与LC-MS/MS比较)符合我们<15%的验收标准，Ser和Tyr的差异更大(分别为19.5%和- 19.0%)，LC-MS/MS中测定的Cit浓度远低于IEC(31%的差异)。

这两种方法对于大多数AAs都具有足够的可比性，除了瓜氨酸外，个别AAs的参考值无需精化。音粗，对于监测治疗中的患者(如苯丙酮尿症患者)，仍然建议始终使用相同的分析方法来定量AAs。

原始出处：

Andraz Smon，Vanja Cuk，Comparison of liquid chromatography with tandem mass spectrometry and ion-exchange chromatography by post-column ninhydrin derivatization for amino acid monitoring

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    2020-03-06 ms3117646317107274

    #测定氨基酸#

    0

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    2020-06-13 丁鹏鹏

    #测定#

    0

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    2019-11-19 zhaohui6731

    #氨基酸#

    0

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    2020-01-17 windight

    #CTA#

    0

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    2019-10-01 最帅小男护

    学习了

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=2076786, encodeId=015c20e67864d, content=<a href='/topic/show?id=bf056545e9b' target=_blank style='color:#2F92EE;'>#测定氨基酸#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65457, encryptionId=bf056545e9b, topicName=测定氨基酸)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/WQ1WyMBGic8d2fKdCe6Cm3vaRh4X8YnlumYndC46iagNCBl9Df8RtoCaQchnFtZldHUctGJjsgsyutUlu8vPq6yQ/132, createdBy=97862500016, createdName=ms3117646317107274, createdTime=Fri Mar 06 04:11:00 CST 2020, time=2020-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1662305, encodeId=bb381662305c1, content=<a href='/topic/show?id=6a426545472' target=_blank style='color:#2F92EE;'>#测定#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65454, encryptionId=6a426545472, topicName=测定)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db925396819, createdName=丁鹏鹏, createdTime=Sat Jun 13 20:11:00 CST 2020, time=2020-06-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1860865, encodeId=e58f186086562, content=<a href='/topic/show?id=5e746403ed5' target=_blank style='color:#2F92EE;'>#氨基酸#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=120, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64037, encryptionId=5e746403ed5, topicName=氨基酸)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=bf3d82, createdName=zhaohui6731, createdTime=Tue Nov 19 14:11:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849752, encodeId=ba9c1849e5250, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=118, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Fri Jan 17 10:11:00 CST 2020, time=2020-01-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373410, encodeId=2d843e3410aa, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/07/01/577acf4668c2eb760152040cdd4c4196.jpg, createdBy=dc3f5107879, createdName=最帅小男护, createdTime=Tue Oct 01 12:46:22 CST 2019, time=2019-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1553367, encodeId=dfdb155336e98, content=<a href='/topic/show?id=81bd923e559' target=_blank style='color:#2F92EE;'>#质谱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=79, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92375, encryptionId=81bd923e559, topicName=质谱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96fd14366106, createdName=jeanqiuqiu, createdTime=Tue Sep 24 08:11:00 CST 2019, time=2019-09-24, status=1, ipAttribution=)]
    2019-09-24 jeanqiuqiu

    #质谱#

    0

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