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Clinica Chimica Acta:在印度北部城市和高度流行的农村人群中,利用基于猪带绦虫囊液的酶联免疫转移印迹技术诊断脑囊虫病的价值

2020-05-30 MedSci原创 MedSci原创

猪脑囊虫病(NCC)是由猪带绦虫引起的。NCC的血清诊断仍然是一个挑战。由于放射成像(CT / MRI)是成本较高,而且需要技术专业知识和特定资源。

猪脑囊虫病(NCC)是由猪带绦虫引起的。NCC的血清诊断仍然是一个挑战。由于放射成像(CT / MRI)是成本较高,而且需要技术专业知识和特定资源。 因此,在地方性地区它的可用性受到限制。现有的酶电免疫转移免疫印迹(EITB)抗原难以制备,也不符合地方性人群的标准。因此,迫切需要简单、可靠的EITB工具。

猪脑囊虫采自屠宰后的自然感染的猪。通过抽吸囊液/囊泡液,超离心处理,免疫印迹法检测。

研究结果显示,总共406例[农村256例(NCC 78,除NCC 108以外的癫痫发作和健康对照者70);纳入城市患者150名(NCC 41,除NCC 59以外的癫痫发作和健康对照50)。 EITB阳性(检测到<50 kDa的条带)仅与城市人口的NCC患者(p <0.001)显著相关,而与农村人口则无显著相关(p = 0.292)。 然而,在城市和农村地区,鉴定15 kDa的条带均与NCC显著相关,总体敏感性为91.5%,特异性为91.6%。 35 kDa条带的存在与多个NCC相关(p <0.001)。研究表明,EITB上的15kda反应带对地方性人群NCC的诊断具有较高的敏感性和特异性。

在EITB上存在35kDa带与多囊尾蚴感染有关。 观察需要纯化囊肿液抗原,以开发简单和易于执行的测试在现场研究。

原始出处:

Naina AroraRimanpreet KaurEvaluation of Taenia solium cyst fluid-based enzyme linked immunoelectro transfer blot for Neurocysticercosis diagnosis in urban and highly endemic rural population of North India

 

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    2020-07-11 windight
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    2020-06-01 daviiliu
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    2020-06-01 muzishouyi
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