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Clinica Chimica Acta:多重rRT-PCR检测SARS-CoV-2 RNA,用于临床实验室对COVID-19的分子诊断

2020-06-22 MedSci原创 MedSci原创

实时逆转录-聚合酶链反应(rRT-PCR)检测SARS-CoV-2 RNA,通过分子诊断实验室确认COVID-19的临床诊断。我们开发了一种用于检测SARS-CoV-2 RNA的多重rRT-PCR方法

实时逆转录-聚合酶链反应(rRT-PCR)检测SARS-CoV-2 RNA,通过分子诊断实验室确认COVID-19的临床诊断。我们开发了一种用于检测SARS-CoV-2 RNA的多重rRT-PCR方法。多重rRT-PCR采用三个基因:sarbecev特异性E基因、SARS-CoV-2特异性N基因和人ABL1基因作为内参。

单纯型和多重型rRT-PCR方法的Cq值具有较好的相关性。采用新型多重rRT-PCR方法可检测SARS-CoV-2 RNA的低拷贝(<25拷贝/反应)。

本研究提出的多重rRT-PCR方法将使SARS-CoV-2 RNA的高灵敏度检测成为可能,减少临床实验室技术人员所需的试剂使用、成本和时间。

原始出处:

Takayuki Ishige,Shota Murata,Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories

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    2020-11-03 changfy
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    2020-08-14 windight
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    2021-01-06 hukaixun
  5. [GetPortalCommentsPageByObjectIdResponse(id=1941568, encodeId=f34a1941568f1, content=<a href='/topic/show?id=108022e76b3' target=_blank style='color:#2F92EE;'>#临床实验#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=22776, encryptionId=108022e76b3, topicName=临床实验)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6779163, createdName=liuhuangbo, createdTime=Fri Mar 26 04:19:47 CST 2021, time=2021-03-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2084008, encodeId=2d04208400808, content=<a href='/topic/show?id=1b2515e2345' target=_blank style='color:#2F92EE;'>#RT-PCR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15723, encryptionId=1b2515e2345, topicName=RT-PCR)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=32d177, createdName=changfy, createdTime=Tue Nov 03 01:19:47 CST 2020, time=2020-11-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849948, encodeId=b4f21849948c8, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Fri Aug 14 22:19:47 CST 2020, time=2020-08-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1937930, encodeId=5a3f193e93088, content=<a href='/topic/show?id=02de15902d3' target=_blank style='color:#2F92EE;'>#SARS-CoV#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15902, encryptionId=02de15902d3, topicName=SARS-CoV)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6ab940, createdName=hukaixun, createdTime=Wed Jan 06 18:19:47 CST 2021, time=2021-01-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1321195, encodeId=cb081321195fb, content=<a href='/topic/show?id=ac0d159007f' target=_blank style='color:#2F92EE;'>#SARS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15900, encryptionId=ac0d159007f, topicName=SARS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8306486, createdName=小刀医生, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1419448, encodeId=2f55141944893, content=<a href='/topic/show?id=e290138682e' target=_blank style='color:#2F92EE;'>#PCR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=119, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13868, encryptionId=e290138682e, topicName=PCR)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=9cc43496966, createdName=Tommy1950, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1585356, encodeId=ca1715853560a, content=<a href='/topic/show?id=7b31138e2ee' target=_blank style='color:#2F92EE;'>#PCR检测#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13872, encryptionId=7b31138e2ee, topicName=PCR检测)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=3bf517165269, createdName=howi, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622914, encodeId=1664162291474, content=<a href='/topic/show?id=517d3168e03' target=_blank style='color:#2F92EE;'>#分子诊断#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31687, encryptionId=517d3168e03, topicName=分子诊断)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=)]
    2020-06-24 小刀医生
  6. [GetPortalCommentsPageByObjectIdResponse(id=1941568, encodeId=f34a1941568f1, content=<a href='/topic/show?id=108022e76b3' target=_blank style='color:#2F92EE;'>#临床实验#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=22776, encryptionId=108022e76b3, topicName=临床实验)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6779163, createdName=liuhuangbo, createdTime=Fri Mar 26 04:19:47 CST 2021, time=2021-03-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2084008, encodeId=2d04208400808, content=<a href='/topic/show?id=1b2515e2345' target=_blank style='color:#2F92EE;'>#RT-PCR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15723, encryptionId=1b2515e2345, topicName=RT-PCR)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=32d177, createdName=changfy, createdTime=Tue Nov 03 01:19:47 CST 2020, time=2020-11-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849948, encodeId=b4f21849948c8, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Fri Aug 14 22:19:47 CST 2020, time=2020-08-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1937930, encodeId=5a3f193e93088, content=<a href='/topic/show?id=02de15902d3' target=_blank style='color:#2F92EE;'>#SARS-CoV#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15902, encryptionId=02de15902d3, topicName=SARS-CoV)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6ab940, createdName=hukaixun, createdTime=Wed Jan 06 18:19:47 CST 2021, time=2021-01-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1321195, encodeId=cb081321195fb, content=<a href='/topic/show?id=ac0d159007f' target=_blank style='color:#2F92EE;'>#SARS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15900, encryptionId=ac0d159007f, topicName=SARS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8306486, createdName=小刀医生, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1419448, encodeId=2f55141944893, content=<a href='/topic/show?id=e290138682e' target=_blank style='color:#2F92EE;'>#PCR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=119, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13868, encryptionId=e290138682e, topicName=PCR)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=9cc43496966, createdName=Tommy1950, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1585356, encodeId=ca1715853560a, content=<a href='/topic/show?id=7b31138e2ee' target=_blank style='color:#2F92EE;'>#PCR检测#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13872, encryptionId=7b31138e2ee, topicName=PCR检测)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=3bf517165269, createdName=howi, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622914, encodeId=1664162291474, content=<a href='/topic/show?id=517d3168e03' target=_blank style='color:#2F92EE;'>#分子诊断#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31687, encryptionId=517d3168e03, topicName=分子诊断)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=)]
    2020-06-24 Tommy1950
  7. [GetPortalCommentsPageByObjectIdResponse(id=1941568, encodeId=f34a1941568f1, content=<a href='/topic/show?id=108022e76b3' target=_blank style='color:#2F92EE;'>#临床实验#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=22776, encryptionId=108022e76b3, topicName=临床实验)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6779163, createdName=liuhuangbo, createdTime=Fri Mar 26 04:19:47 CST 2021, time=2021-03-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2084008, encodeId=2d04208400808, content=<a href='/topic/show?id=1b2515e2345' target=_blank style='color:#2F92EE;'>#RT-PCR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15723, encryptionId=1b2515e2345, topicName=RT-PCR)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=32d177, createdName=changfy, createdTime=Tue Nov 03 01:19:47 CST 2020, time=2020-11-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849948, encodeId=b4f21849948c8, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Fri Aug 14 22:19:47 CST 2020, time=2020-08-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1937930, encodeId=5a3f193e93088, content=<a href='/topic/show?id=02de15902d3' target=_blank style='color:#2F92EE;'>#SARS-CoV#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15902, encryptionId=02de15902d3, topicName=SARS-CoV)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6ab940, createdName=hukaixun, createdTime=Wed Jan 06 18:19:47 CST 2021, time=2021-01-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1321195, encodeId=cb081321195fb, content=<a href='/topic/show?id=ac0d159007f' target=_blank style='color:#2F92EE;'>#SARS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15900, encryptionId=ac0d159007f, topicName=SARS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8306486, createdName=小刀医生, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1419448, encodeId=2f55141944893, content=<a href='/topic/show?id=e290138682e' target=_blank style='color:#2F92EE;'>#PCR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=119, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13868, encryptionId=e290138682e, topicName=PCR)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=9cc43496966, createdName=Tommy1950, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1585356, encodeId=ca1715853560a, content=<a href='/topic/show?id=7b31138e2ee' target=_blank style='color:#2F92EE;'>#PCR检测#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13872, encryptionId=7b31138e2ee, topicName=PCR检测)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=3bf517165269, createdName=howi, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622914, encodeId=1664162291474, content=<a href='/topic/show?id=517d3168e03' target=_blank style='color:#2F92EE;'>#分子诊断#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31687, encryptionId=517d3168e03, topicName=分子诊断)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Wed Jun 24 14:19:47 CST 2020, time=2020-06-24, status=1, ipAttribution=)]
    2020-06-24 howi
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