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Dent Mater J：酸蚀过牙本质洞壁的内源性基质金属蛋白酶含量的评估

2019-09-18 lishiting MedSci原创

MMP水平牙本质
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研究显示，内源性牙本质基质金属蛋白酶(MMPs)与牙本质粘接的树脂渗透层内胶原纤维自主降解相关联。为了靶向这些水解蛋白酶，我们有必要掌握在树脂/牙本质界面会激活和产生何种MMPs。

研究显示，内源性牙本质基质金属蛋白酶(MMPs)与牙本质粘接的树脂渗透层内胶原纤维自主降解相关联。为了靶向这些水解蛋白酶，我们有必要掌握在树脂/牙本质界面会激活和产生何种MMPs。在这篇研究中，作用从单颗拔除牙齿窝洞壁提取牙本质样本内源性MMPs的定量水平和提取程序进行优化。在作者构建的牙齿窝洞模型中，预备1个合面窝洞(2×4×2 mm)并从冠方去除，提取围绕周围的1-mm厚度牙本质壁。采用粉碎仪将样本制备成粉末状。酶联免疫吸附测定(ELISA)结果显示，在不到300 mg牙本质粉末中检测到平均34.7 pg的MMP-9。这是第一篇通过此种方法定量检测从单颗拔除牙齿窝洞壁分离出来的牙本质蛋白内的内源性MMP水平。原始出处：Kang J， Izutani N， et al. Assaying endogenous matrix metalloproteinases (MMPs) in acid-etched dentinal cavity walls. Dent Mater J. 2019 Sep 11. doi： 10.4012/dmj.2018-342.本文系梅斯医学（MedSci）

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    2019-10-01 canlab

    #蛋白酶#

    0

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    2020-01-10 小华子

    #基质金属蛋白酶#

    0

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    2019-09-25 ms6897892027066920

    #金属蛋白酶#

    0

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    2020-01-17 diushouji

    #内源性#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1945215, encodeId=9533194521585, content=<a href='/topic/show?id=249488e60fe' target=_blank style='color:#2F92EE;'>#蛋白酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88760, encryptionId=249488e60fe, topicName=蛋白酶)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Tue Oct 01 11:22:00 CST 2019, time=2019-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2054464, encodeId=f2c32054464d3, content=<a href='/topic/show?id=bd9b4218475' target=_blank style='color:#2F92EE;'>#基质金属蛋白酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42184, encryptionId=bd9b4218475, topicName=基质金属蛋白酶)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTKCRibWHQArPHdSkqP90U1HwwqlGeU7BUgzY240D3v2ibBDJ09ybkGkwOjxz7qRmngZaGlK1taX6hfA/132, createdBy=2e772500094, createdName=小华子, createdTime=Fri Jan 10 06:22:00 CST 2020, time=2020-01-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2019916, encodeId=4290201991658, content=<a href='/topic/show?id=5a6d961841b' target=_blank style='color:#2F92EE;'>#金属蛋白酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96184, encryptionId=5a6d961841b, topicName=金属蛋白酶)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTLc3PoqaNe4QTZ947pojXuchvvmkkJN0wyBBbA3LVX2WDkgiaJ7cswu9yvA4CB0NicjcrCfBY1zRPkA/132, createdBy=b3812500089, createdName=ms6897892027066920, createdTime=Wed Sep 25 08:22:00 CST 2019, time=2019-09-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1682037, encodeId=ab6c168203e90, content=<a href='/topic/show?id=15593040e9a' target=_blank style='color:#2F92EE;'>#内源性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=30407, encryptionId=15593040e9a, topicName=内源性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b46b27929604, createdName=diushouji, createdTime=Fri Jan 17 20:22:00 CST 2020, time=2020-01-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1757189, encodeId=acf11e57189c5, content=<a href='/topic/show?id=103b421e0e8' target=_blank style='color:#2F92EE;'>#基质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42170, encryptionId=103b421e0e8, topicName=基质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=281337296540, createdName=ms7899726347904398, createdTime=Thu Jul 23 23:22:00 CST 2020, time=2020-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1441963, encodeId=5a5e144196301, content=<a href='/topic/show?id=67526e55924' target=_blank style='color:#2F92EE;'>#牙本质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67559, encryptionId=67526e55924, topicName=牙本质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=37364903098, createdName=ms8538335253054589, createdTime=Fri Sep 20 12:22:00 CST 2019, time=2019-09-20, status=1, ipAttribution=)]
    2020-07-23 ms7899726347904398

    #基质#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1945215, encodeId=9533194521585, content=<a href='/topic/show?id=249488e60fe' target=_blank style='color:#2F92EE;'>#蛋白酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88760, encryptionId=249488e60fe, topicName=蛋白酶)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Tue Oct 01 11:22:00 CST 2019, time=2019-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2054464, encodeId=f2c32054464d3, content=<a href='/topic/show?id=bd9b4218475' target=_blank style='color:#2F92EE;'>#基质金属蛋白酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42184, encryptionId=bd9b4218475, topicName=基质金属蛋白酶)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTKCRibWHQArPHdSkqP90U1HwwqlGeU7BUgzY240D3v2ibBDJ09ybkGkwOjxz7qRmngZaGlK1taX6hfA/132, createdBy=2e772500094, createdName=小华子, createdTime=Fri Jan 10 06:22:00 CST 2020, time=2020-01-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2019916, encodeId=4290201991658, content=<a href='/topic/show?id=5a6d961841b' target=_blank style='color:#2F92EE;'>#金属蛋白酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96184, encryptionId=5a6d961841b, topicName=金属蛋白酶)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTLc3PoqaNe4QTZ947pojXuchvvmkkJN0wyBBbA3LVX2WDkgiaJ7cswu9yvA4CB0NicjcrCfBY1zRPkA/132, createdBy=b3812500089, createdName=ms6897892027066920, createdTime=Wed Sep 25 08:22:00 CST 2019, time=2019-09-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1682037, encodeId=ab6c168203e90, content=<a href='/topic/show?id=15593040e9a' target=_blank style='color:#2F92EE;'>#内源性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=30407, encryptionId=15593040e9a, topicName=内源性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b46b27929604, createdName=diushouji, createdTime=Fri Jan 17 20:22:00 CST 2020, time=2020-01-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1757189, encodeId=acf11e57189c5, content=<a href='/topic/show?id=103b421e0e8' target=_blank style='color:#2F92EE;'>#基质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42170, encryptionId=103b421e0e8, topicName=基质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=281337296540, createdName=ms7899726347904398, createdTime=Thu Jul 23 23:22:00 CST 2020, time=2020-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1441963, encodeId=5a5e144196301, content=<a href='/topic/show?id=67526e55924' target=_blank style='color:#2F92EE;'>#牙本质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67559, encryptionId=67526e55924, topicName=牙本质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=37364903098, createdName=ms8538335253054589, createdTime=Fri Sep 20 12:22:00 CST 2019, time=2019-09-20, status=1, ipAttribution=)]
    2019-09-20 ms8538335253054589

    #牙本质#

    0

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