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J Endod:动态冲洗促进未发育完成根管模型中根尖牙乳头细胞的体外粘附

2018-03-23 lishiting MedSci原创

目前,牙本质预处理技术的应用在牙髓再生治疗中受到推崇。牙本质在EDTA超声冲洗后可以检测到数种生长因子。这篇研究的目的是为了评估采用多种不同溶液方案实施动态冲洗对未发育完成牙齿模型中根尖牙乳头细胞(APC)体外粘附的影响。

目前,牙本质预处理技术的应用在牙髓再生治疗中受到推崇。牙本质在EDTA超声冲洗后可以检测到数种生长因子。这篇研究的目的是为了评估采用多种不同溶液方案实施动态冲洗对未发育完成牙齿模型中根尖牙乳头细胞(APC)体外粘附的影响。

研究采用不同的动态冲洗技术:指针冲洗(NI)、NI+ EndoActivator和NI+被动超声冲洗,配合使用不同的冲洗溶液:生理盐水(NSS)、EDTA和氯己定二葡糖酸盐+EDTA,对插入含氢氧化钙牙本质片的已扩大根管模型实施动态冲洗。对最初粘附的纤连蛋白阳性APC的数量进行计数,并通过扫描电镜检查牙本质表面形态。

结果显示,动态冲洗组的APCs数量要明显高于对照组(P < .001)。在应用相同冲洗技术时,使用NSS溶液组的APCs数量高于使用EDTA或氯己定二葡糖酸盐+EDTA组(P < .001)。根管内所有位置的细胞数量均相似;然而,使用NSS的超声组在根管根中和根尖部的粘附细胞数量明显增加(P < .05)。

结论:在未发育完成牙齿模型中使用动态冲洗法可以显著促进APC粘附于含氢氧化钙的牙本质上。另外,当NSS作为终冲洗溶液时,粘附细胞的数量显著增加。

原始出处

Prompreecha S, Sastraruji T, et al. Dynamic Irrigation Promotes Apical Papilla Cell Attachment in an Ex Vivo Immature Root Canal Model. J Endod. 2018 Mar 14. pii: S0099-2399(18)30047-5. doi: 10.1016/j.joen.2018.01.012.

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  2. [GetPortalCommentsPageByObjectIdResponse(id=1739931, encodeId=d6421e3993108, content=<a href='/topic/show?id=f49c310e134' target=_blank style='color:#2F92EE;'>#冲洗#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31071, encryptionId=f49c310e134, topicName=冲洗)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=4ec334602181, createdName=ms3892185887221420, createdTime=Fri Aug 03 20:38:00 CST 2018, time=2018-08-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347276, encodeId=67dd134e276aa, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522885, encodeId=61211522885a7, content=<a href='/topic/show?id=c72462241ff' target=_blank style='color:#2F92EE;'>#根管#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62241, encryptionId=c72462241ff, topicName=根管)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ba5e11492705, createdName=zhaozhouchifen, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298972, encodeId=5d372989e257, content=我也想投.但是看到您写的这个.所以问下.我也是第一次听说, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b2d52278456, createdName=121bde57m15(暂无昵称), createdTime=Fri Mar 23 14:39:01 CST 2018, time=2018-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298929, encodeId=652b298929f8, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Fri Mar 23 09:46:12 CST 2018, time=2018-03-23, status=1, ipAttribution=)]
    2018-03-25 neurowu
  3. [GetPortalCommentsPageByObjectIdResponse(id=1739931, encodeId=d6421e3993108, content=<a href='/topic/show?id=f49c310e134' target=_blank style='color:#2F92EE;'>#冲洗#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31071, encryptionId=f49c310e134, topicName=冲洗)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=4ec334602181, createdName=ms3892185887221420, createdTime=Fri Aug 03 20:38:00 CST 2018, time=2018-08-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347276, encodeId=67dd134e276aa, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522885, encodeId=61211522885a7, content=<a href='/topic/show?id=c72462241ff' target=_blank style='color:#2F92EE;'>#根管#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62241, encryptionId=c72462241ff, topicName=根管)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ba5e11492705, createdName=zhaozhouchifen, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298972, encodeId=5d372989e257, content=我也想投.但是看到您写的这个.所以问下.我也是第一次听说, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b2d52278456, createdName=121bde57m15(暂无昵称), createdTime=Fri Mar 23 14:39:01 CST 2018, time=2018-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298929, encodeId=652b298929f8, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Fri Mar 23 09:46:12 CST 2018, time=2018-03-23, status=1, ipAttribution=)]
  4. [GetPortalCommentsPageByObjectIdResponse(id=1739931, encodeId=d6421e3993108, content=<a href='/topic/show?id=f49c310e134' target=_blank style='color:#2F92EE;'>#冲洗#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31071, encryptionId=f49c310e134, topicName=冲洗)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=4ec334602181, createdName=ms3892185887221420, createdTime=Fri Aug 03 20:38:00 CST 2018, time=2018-08-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347276, encodeId=67dd134e276aa, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522885, encodeId=61211522885a7, content=<a href='/topic/show?id=c72462241ff' target=_blank style='color:#2F92EE;'>#根管#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62241, encryptionId=c72462241ff, topicName=根管)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ba5e11492705, createdName=zhaozhouchifen, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298972, encodeId=5d372989e257, content=我也想投.但是看到您写的这个.所以问下.我也是第一次听说, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b2d52278456, createdName=121bde57m15(暂无昵称), createdTime=Fri Mar 23 14:39:01 CST 2018, time=2018-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298929, encodeId=652b298929f8, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Fri Mar 23 09:46:12 CST 2018, time=2018-03-23, status=1, ipAttribution=)]
    2018-03-23 121bde57m15(暂无昵称)

    我也想投.但是看到您写的这个.所以问下.我也是第一次听说

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1739931, encodeId=d6421e3993108, content=<a href='/topic/show?id=f49c310e134' target=_blank style='color:#2F92EE;'>#冲洗#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31071, encryptionId=f49c310e134, topicName=冲洗)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=4ec334602181, createdName=ms3892185887221420, createdTime=Fri Aug 03 20:38:00 CST 2018, time=2018-08-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347276, encodeId=67dd134e276aa, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522885, encodeId=61211522885a7, content=<a href='/topic/show?id=c72462241ff' target=_blank style='color:#2F92EE;'>#根管#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62241, encryptionId=c72462241ff, topicName=根管)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ba5e11492705, createdName=zhaozhouchifen, createdTime=Sun Mar 25 00:38:00 CST 2018, time=2018-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298972, encodeId=5d372989e257, content=我也想投.但是看到您写的这个.所以问下.我也是第一次听说, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b2d52278456, createdName=121bde57m15(暂无昵称), createdTime=Fri Mar 23 14:39:01 CST 2018, time=2018-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=298929, encodeId=652b298929f8, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Fri Mar 23 09:46:12 CST 2018, time=2018-03-23, status=1, ipAttribution=)]
    2018-03-23 龙胆草

    学习谢谢分享

    0

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