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Sci Rep:外泌体在单纯性脂肪肝进展到NASH中发挥重要作用

2017-08-05 MedSci MedSci原创

非酒精性脂肪性肝病(NAFLD)是慢性肝病发生的主要病因,但是从单纯性脂肪变性到非酒精性脂肪性肝炎(NASH)的确切机制目前尚不清楚。本研究中研究人员探究了外泌体在NAFLD进展中的作用。研究人员使用棕榈酸(PA)分离人肝癌细胞株分离出外泌体,并使用miRNA芯片检测miRNA。然后使用从肝细胞分离的外泌体处理肝星状细胞(HSC)系(LX-2)。与对照组相比,PA处理的肝细胞CD36得表达水平显著

非酒精性脂肪性肝病(NAFLD)是慢性肝病发生的主要病因,但是从单纯性脂肪变性到非酒精性脂肪性肝炎(NASH)的确切机制目前尚不清楚。本研究中研究人员探究了外泌体在NAFLD进展中的作用。

研究人员使用棕榈酸(PA)分离人肝癌细胞株分离出外泌体,并使用miRNA芯片检测miRNA。然后使用从肝细胞分离的外泌体处理肝星状细胞(HSC)系(LX-2)。与对照组相比,PA处理的肝细胞CD36得表达水平显著升高,且有外泌体的产生。微阵列分析显示,来源于赋形剂与来源于PA处理的干细胞的外泌体内miRNA的表达存在明显的差别。与使用赋形剂处理肝细胞的外泌体与LX-2细胞共培养相比,使用PA处理肝细胞的外泌体与LX-2细胞共培养时,与肝细胞纤维化发生相关的基因表达明显增多。

总之,该研究表明,PA处理肝细胞可显著增强Exosomes的产生,并会改变外泌内miRNA的表达谱。此外,来自PA处理的肝细胞的外泌体可引起HSCs内纤维化基因表达水平的增加。因此,外泌体可能在单纯性脂肪肝进展到NASH的过程中起着非常重要的作用。

原始出处:

Young-Sun Lee, So Yeon Kim, et al., Exosomes derived from palmitic acid-treated hepatocytes induce fibrotic activation of hepatic stellate cells. Sci Rep. 2017; 7: 3710. Published online 2017 Jun 16. doi: 10.1038/s41598-017-03389-2.

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    2017-08-07 kksonne
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    2017-08-06 执着追梦

    学习,谢谢分享

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=2023487, encodeId=ebff202348ee5, content=<a href='/topic/show?id=57ce9605ef6' target=_blank style='color:#2F92EE;'>#重要作用#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=94, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96057, encryptionId=57ce9605ef6, topicName=重要作用)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8e4c53, createdName=jiyangfei, createdTime=Fri Jan 19 06:19:00 CST 2018, time=2018-01-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1301181, encodeId=804813011819a, content=<a href='/topic/show?id=38e32852fe' target=_blank style='color:#2F92EE;'>#ASH#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2852, encryptionId=38e32852fe, topicName=ASH)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=39a0295, createdName=kksonne, createdTime=Mon Aug 07 13:19:00 CST 2017, time=2017-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=230587, encodeId=8faa23058ed6, content=学习,谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sun Aug 06 07:16:55 CST 2017, time=2017-08-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=230549, encodeId=237223054915, content=学习了谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5Kzuf9u71gZhPYMiajRtENwicoAABeQtfXOlic8ibhYSy6DvJZWUDVtjRvTfqBffr1XJ6JLtFB5kHicthl/0, createdBy=08bb2061153, createdName=189****7206, createdTime=Sun Aug 06 07:05:04 CST 2017, time=2017-08-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=230462, encodeId=038a230462ee, content=来自PA处理的肝细胞的外泌体可引起HSCs内纤维化基因表达水平的增加。因此,外泌体可能在单纯性脂肪肝进展到NASH的过程中起着非常重要的作用。, beContent=null, objectType=article, channel=null, level=null, likeNumber=112, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7cJ6ejWUr2nIia8qOvAKNdLHr32Df6G8iaoaOx6pHaoiacwp26DaslxZHCGbibZhhCGven4rvmWP60GahtzgFjgCjR/0, createdBy=6b111703011, createdName=有备才能无患, createdTime=Sat Aug 05 22:27:21 CST 2017, time=2017-08-05, status=1, ipAttribution=)]
    2017-08-06 189****7206

    学习了谢谢分享

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=2023487, encodeId=ebff202348ee5, content=<a href='/topic/show?id=57ce9605ef6' target=_blank style='color:#2F92EE;'>#重要作用#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=94, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96057, encryptionId=57ce9605ef6, topicName=重要作用)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8e4c53, createdName=jiyangfei, createdTime=Fri Jan 19 06:19:00 CST 2018, time=2018-01-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1301181, encodeId=804813011819a, content=<a href='/topic/show?id=38e32852fe' target=_blank style='color:#2F92EE;'>#ASH#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2852, encryptionId=38e32852fe, topicName=ASH)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=39a0295, createdName=kksonne, createdTime=Mon Aug 07 13:19:00 CST 2017, time=2017-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=230587, encodeId=8faa23058ed6, content=学习,谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sun Aug 06 07:16:55 CST 2017, time=2017-08-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=230549, encodeId=237223054915, content=学习了谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5Kzuf9u71gZhPYMiajRtENwicoAABeQtfXOlic8ibhYSy6DvJZWUDVtjRvTfqBffr1XJ6JLtFB5kHicthl/0, createdBy=08bb2061153, createdName=189****7206, createdTime=Sun Aug 06 07:05:04 CST 2017, time=2017-08-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=230462, encodeId=038a230462ee, content=来自PA处理的肝细胞的外泌体可引起HSCs内纤维化基因表达水平的增加。因此,外泌体可能在单纯性脂肪肝进展到NASH的过程中起着非常重要的作用。, beContent=null, objectType=article, channel=null, level=null, likeNumber=112, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7cJ6ejWUr2nIia8qOvAKNdLHr32Df6G8iaoaOx6pHaoiacwp26DaslxZHCGbibZhhCGven4rvmWP60GahtzgFjgCjR/0, createdBy=6b111703011, createdName=有备才能无患, createdTime=Sat Aug 05 22:27:21 CST 2017, time=2017-08-05, status=1, ipAttribution=)]
    2017-08-05 有备才能无患

    来自PA处理的肝细胞的外泌体可引起HSCs内纤维化基因表达水平的增加。因此,外泌体可能在单纯性脂肪肝进展到NASH的过程中起着非常重要的作用。

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外泌体的最重要的生物学功能是它们可能用作临床诊断中的生物标志物。与在常规标本例如血清或尿中鉴定的生物标志物相比,外泌体生物标志物提供最高量的灵敏度和特异性,这可归因于它们优异的稳定性。这里梅斯小编整理了近期今年以来关于外泌体的重要研究进展与大家一同分享。【1】Nature:外泌体还能用于胰腺癌治疗6月7日Nature在线发表了安德森癌症中心Raghu Kalluri教授新的研究进展:修饰过后的

SCI REP:外泌体中的miRNA可作为川崎病诊断标志

川崎病(Kawasaki disease),是一种以全身血管炎变为主要病理的急性发热性出疹性小儿疾病。虽然已有的研究已经发现一些血来源的蛋白分子或者miRNA有潜在的诊断能力,但是,其有效性还有待提高。外泌体,一种包含了细胞特异性信息的囊泡结构,保护了内部的分子免受胞外酶类的降解。因此,通过检测外泌体中的遗传物质,是寻找可靠诊断biomarker的理想方式。

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