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J Endod：冲洗液对人的根尖牙乳头干细胞的影响

2017-12-20 lishiting MedSci原创

Endocyn细胞毒性
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Endocyn为一种pH中性的次氯酸和次氯酸盐溶液，已经得到开发并应用于根管冲洗。这篇研究的目的是为了评估Endocyn对人牙周膜(PDL)成纤维细胞、大鼠骨肉瘤细胞(UMR-106)和根尖牙乳头干细胞(SCAP)的影响，并与其它常用冲洗液相比较。

Endocyn为一种pH中性的次氯酸和次氯酸盐溶液，已经得到开发并应用于根管冲洗。这篇研究的目的是为了评估Endocyn对人牙周膜(PDL)成纤维细胞、大鼠骨肉瘤细胞(UMR-106)和根尖牙乳头干细胞(SCAP)的影响，并与其它常用冲洗液相比较。对于细胞毒性的检测，研究将细胞暴露于不同浓度的Endocyn， 6% NaOCl， 17% EDTA和2%氯已定10分钟、1小时或24小时。使用钙黄绿素AM荧光观察细胞存活情况。另外，也检测Endocyn抑制SCAP增殖和碱性磷酸酶活性的能力。最后，通过反转录聚合酶链式反应检测SCAP的转录表达变化。结果显示，在施加刺激至24小时时，Endocyn对PDL和UMR细胞表现出来的毒性不比水多。50%的Endocyn浓度对SCAP刺激1小时展现出了一定的毒性。Endocyn浓度>20%会抑制SCAP增殖，而浓度≥10%会抑制碱性磷酸酶活性。10% Endocyn暴露于SCAP 3天并未改变大多数的转录表达，但却明显减弱了碱性磷酸酶活性、纤维调节素和osteomodulin的表达。结论：与其它常用的根管冲洗液相比，Endocyn展现出对PDL

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    2018-04-24 ms3892185887221420

    #冲洗#

    0

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    2018-09-16 ms920641618706883

    #冲洗液#

    0

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    2017-12-20 hhh678

    henhao

    0

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    2017-12-20 太上老君1968

    henhao

    0

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