Sci Rep：乳腺癌中钙化基因的表达谱

2017-09-22 MedSci MedSci原创

本研究中，研究人员调查了乳腺癌中钙化的基因表达谱。使用AffymetrixGeneChip®人类基因2.0 ST芯片对168例乳腺癌患者的手术样本进行基因表达分析。钼靶X线钙化由三位放射科医师进行审查，根据恶性肿瘤概率分为三组：乳腺癌无可疑钙化；低至中度可疑钙化的乳腺癌；和高度可疑钙化的乳腺癌。为了鉴定这三组之间差异表达的基因（DEGs），研究人员使用Tukey的可靠显著差异法的事后对比分析进行单

本研究中，研究人员调查了乳腺癌中钙化的基因表达谱。使用AffymetrixGeneChip®人类基因2.0 ST芯片对168例乳腺癌患者的手术样本进行基因表达分析。钼靶X线钙化由三位放射科医师进行审查，根据恶性肿瘤概率分为三组：乳腺癌无可疑钙化；低至中度可疑钙化的乳腺癌；和高度可疑钙化的乳腺癌。

为了鉴定这三组之间差异表达的基因（DEGs），研究人员使用Tukey HSD 事后对比分析进行单因素方差分析。为了探究DEGs的生物学意义，研究人员使用DAVID进行基因本体分析和BioLattice进行聚类分析。

结果，研究总发现2551个基因在三组间差异表达。高度可疑钙化的乳腺癌中ERBB2基因的表达上调（倍数变化2.474，p <0.001）。基因本体分析显示，与没有可疑钙化的乳腺癌相比，具有高度可疑钙化的乳腺癌的免疫、防御和炎症反应降低（p从10-23到10-8）。聚类分析也表明，免疫系统与钼靶X线相关（p <0.001）。

总之，该研究结果表明，乳腺癌的钙化与ERBB2的mRNA高水平表达及免疫系统活性降低有关。

原始出处：

Sung Ui Shin, Jeonghoon Lee, et al., Gene expression profiling of calcifications in breast cancer. Sci Rep. 2017; 7: 11427. Published online 2017 Sep 12. doi: 10.1038/s41598-017-11331-9.

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2017-09-29 zhangqingzui

好好学习.感谢

136 0
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2017-09-28 zhangqingzui

好好学习.感谢

112 0
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2017-09-25 zhangqingzui

好好学习.感谢

83 0
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2017-09-23 zhangqingzui

好好学习.感谢

126 0
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2017-09-22 135****7952平儿

学了..........

100 0
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2017-09-22 komasun

学习了谢谢分享

85 0
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2017-09-22 komasun

学习了谢谢分享

65 0
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2017-09-22 zhangqingzui

好好学习.感谢

77 0

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