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PLoS Med:Xpert——埃博拉病毒检测新方法!

2016-03-31 zhaozhou 译 MedSci原创

 埃博拉病毒在西非国家大肆传播,野外实验室检测埃博拉病毒依赖于复杂的、多步骤的实时逆转录PCR检测;一种精确的RT-PCR检测将减少检测病毒的时间,并且有可能增加检测效率。来自英国、美国、塞拉利昂等国的科研人员评价了一中检测埃博拉病毒的方法Cepheid GeneXpert的性能。这种方法通过静脉穿刺获得全血(WB)和口腔粘膜(BS)测试样本。 此项研究在公共卫生英格兰EVD检

埃博拉病毒在西非国家大肆传播,野外实验室检测埃博拉病毒依赖于复杂的、多步骤的实时逆转录PCR检测;一种精确的RT-PCR检测将减少检测病毒的时间,并且有可能增加检测效率。来自英国、美国、塞拉利昂等国的科研人员评价了一中检测埃博拉病毒的方法Cepheid GeneXpert的性能。这种方法通过静脉穿刺获得全血(WB)和口腔粘膜(BS)测试样本。
 

此项研究在公共卫生英格兰EVD检测实验室进行,使用经过临床检测的剩余的检测样本。EDTA-WB样本(n=218)在2015年4月1日到7月20日之间从可疑的或者确定的埃博拉病毒感染者中收集得到。EDTA-WB和BS样本使用Xpert(靶标:糖蛋白和核蛋白基因)和Trombley(靶标:核蛋白基因)平行检测。所有的WB杨蓓均是新鲜的。在所有测试样本中,7/218(3.2%)WB和7/71(9.9%)BS样本有Xpert结果,报道为“不确定”或者“错误”并且被排除在外,留下了211个WB和64个BS有着确定的Trombley和Xpert结果的样本。对于WB,22/22Trombley阳性样本均为Xpert阳性(灵敏度100%),181/189个Trombley阴性样本为Xpert阴性(特异性95.8%)。Xpert方法检测埃博拉病毒展现出了良好的性能,但是对于在使用前已经冰冻的样本来说,这种检测是 受限的。
 
通过此项研究,Xpert方法检测埃博拉病毒被证明是一种高效的方法,可以用于野外实验室检测埃博拉病毒感染。

原始出处:

Semper AE, Broadhurst MJ, Richards J, Foster GM, Simpson AJH, et al. (2016) Performance of the GeneXpert Ebola Assay for Diagnosis of Ebola Virus Disease in Sierra Leone: A Field Evaluation Study. PLoS Med 13(3): e1001980. doi: 10.1371/journal.pmed.1001980

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    2016-10-12 feather89
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  6. 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  7. 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    2016-04-01 lovejing

    灵敏度这么高?

    0

  8. 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likeNumber=156, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9e0b1603164, createdName=lovejing, createdTime=Fri Apr 01 19:15:00 CST 2016, time=2016-04-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=75167, encodeId=38f5e5167c1, content=灵敏度这么高?, beContent=null, objectType=article, channel=null, level=null, likeNumber=157, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9e0b1603164, createdName=lovejing, createdTime=Fri Apr 01 19:15:00 CST 2016, time=2016-04-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=75168, encodeId=f0dae516869, content=灵敏度这么高?, beContent=null, objectType=article, channel=null, level=null, likeNumber=186, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9e0b1603164, createdName=lovejing, createdTime=Fri Apr 01 19:15:00 CST 2016, time=2016-04-01, status=1, ipAttribution=)]
    2016-04-01 lovejing

    灵敏度这么高?

    0

  9. 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    2016-04-01 lovejing

    灵敏度这么高?

    0

  10. 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createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9e0b1603164, createdName=lovejing, createdTime=Fri Apr 01 19:15:00 CST 2016, time=2016-04-01, status=1, ipAttribution=)]
    2016-04-01 lovejing

    灵敏度这么高?

    0

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