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J Periodontol:超声洁治VS.激光治疗种植体周围炎的效果

2018-02-05 MedSci MedSci原创

本研究旨在评估常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响:1)牙菌斑和生物腐蚀氧化钛涂层的去除;2)表面改变诱导;和3)对成骨细胞的生物相容性。将龈下菌斑涂覆在适度粗糙表面的钛片,然后乙醇固定,并且以非接触模式用超声波洁牙机(金属尖端)或Er:YAG激光(20.3或38.2J/cm 2)处理。荧光检测残留菌斑。通过扫描电子显微镜评估钛片表面形态

本研究旨在评估常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响:1)牙菌斑和生物腐蚀氧化钛涂层的去除;2)表面改变诱导;和3)对成骨细胞的生物相容性。

将龈下菌斑涂覆在适度粗糙表面的钛片,然后乙醇固定,并且以非接触模式用超声波洁牙机(金属尖端)或Er:YAG激光(20.3或38.2J/cm 2)处理。荧光检测残留菌斑。通过扫描电子显微镜评估钛片表面形态。通过碘化丙锭/DNA染色试验分析Saos-2成骨细胞在新的和处理过的钛片表面的生存能力、附着、增殖和分化;共聚焦显微镜分析细胞骨架、Ki67、骨桥蛋白和碱性磷酸酶的表达以及矿化结节的形成。

两种方法都能有效地清洁钛片表面,超声波洁牙机可使斑块面积减少到11.7%,Er:YAG激光(38.2 J/cm2)可减少到≤0.03%。超声处理的钛片表面表现出明显的变化,二氧化钛(TiO2)层的不完全去除和少量斑块残留,而Er:YAG激光(38.2J/cm 2)可完全去除斑块和TiO2层,开放钛表面微观结构。两种处理都保持Saos-2成骨细胞表面良好的生物相容性。气-水冷却保持钛片温度低于47℃的临界阈值。

综上所述,这项研究表明,具有金属尖端的超声波洁牙机在去除阳极氧化钛片上的斑块和TiO2层方面不如高能量Er:YAG激光的效果好,虽然两种方法似乎能够恢复处理过的钛表面足够的骨导电率。

原始出处:

Giannelli M, Bani D, et al., Effects of an Erbium:Yttrium-Aluminum-Garnet Laser and Ultrasonic Scaler on Titanium Dioxide-Coated Titanium Surfaces Contaminated With Subgingival Plaque: An In Vitro Study to Assess Post-Treatment Biocompatibility With Osteogenic Cells. J Periodontol. 2017 Nov;88(11):1211-1220. doi: 10.1902/jop.2017.170195. Epub 2017 Jul 10.

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    2019-01-15 hyf028
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    2018-07-27 feather89
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    2018-07-25 huangdf
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    2018-02-07 oliver169
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