Methods Mol Biol：人类动物模型视网膜色素上皮-脉络膜外植体培养物综述

2018-11-19 MedSci MedSci原创

德国基尔大学医学中心眼科的Klettner A近日在Methods Mol Biol杂志上发表了一篇文章，他们以猪为主要的人类RPE动物模型，详细论述了视网膜色素上皮（RPE）-脉络膜外植体目前的研究状况。

德国基尔大学医学中心眼科的Klettner A近日在Methods Mol Biol杂志上发表了一篇文章，他们以猪为主要的人类RPE动物模型，详细论述了视网膜色素上皮（RPE）-脉络膜外植体目前的研究状况。

视网膜色素上皮（RPE）-脉络膜外植体的培养实验可以使得研究者在其自然环境下研究RPE和布鲁赫膜。猪眼睛很容易获得，是人类RPE的良好动物模型。从处死的猪中制备外植体，并且要在4个小时内制备完成，并将其转移到固定环中。制备的外植体可以在环之间的组织中培养，并在灌注器官培养系统中可以培养长达一周。同时可以通过钙黄绿素染色来研究外植体的存活力等情况。

总之，这篇综述为人类理解视网膜色素上皮-脉络膜相关疾病整理了思路。

原文出处：

Klettner， A. and Y. Miura， Porcine RPE/Choroidal Explant Cultures. Methods Mol Biol， 2019. 1834： p. 109-118

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2019-07-06 12498ebem31暂无昵称

#Biol#

50 0
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2019-10-19 12498568m59暂无昵称

#脉络膜#

72 0
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2019-06-06 一闲

#MET#

66 0
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2019-07-28 weihongyv

#色素上皮#

74 0
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2019-04-17 sunylz

#Bio#

50 0
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2019-10-31 sunylz

#色素#

46 0
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2018-11-21 zutt

#视网膜#

53 0
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2018-11-19 CHANGE

梅斯里提供了很多疾病的模型计算公式，赞一个！

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