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Lasers Med Sci:蓝光发光二极管的照射增强了体外牙龈间充质干细胞的成骨分化

2019-03-04 不详 网络

本研究旨在探究蓝光照射对干细胞成骨分化过程的影响。使用的细胞来自人牙龈间充质干细胞(hGMSC),并使用蓝色发光二极管用0(对照组),1,2,4或6J /cm 2蓝光处理。通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴化物(MTT)细胞增殖试验评估细胞生长,并通过监测碱性磷酸酶(ALP)活性来评估成骨分化,茜素红染色和实时PCR(RT-PCR)。 MTT测定的结果表明蓝光抑制

本研究旨在探究蓝光照射对干细胞成骨分化过程的影响。

使用的细胞来自人牙龈间充质干细胞(hGMSC),并使用蓝色发光二极管用0(对照组),1,2,4或6J /cm 2蓝光处理。通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴化物(MTT)细胞增殖试验评估细胞生长,并通过监测碱性磷酸酶(ALP)活性来评估成骨分化,茜素红染色和实时PCR(RT-PCR)。

MTT测定的结果表明蓝光抑制hGMSC增殖,ALP和茜素红结果显示蓝光促进成骨。当细胞用2或4J /cm 2的蓝光照射时,成骨基因runt相关转录因子2(Runx2),I型胶原(Col1)和骨钙蛋白(OCN)的表达水平显著增加(P <0.05)。

总之,该研究结果表明,蓝光照射抑制hGMSC的增殖并促进成骨分化。

原始出处:

Zhu T, Wu Y, et al., Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro. Lasers Med Sci. 2019 Mar 2. doi: 10.1007/s10103-019-02750-3.

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    2019-06-21 HinsMax
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    2019-12-21 anminleiryan
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  7. [GetPortalCommentsPageByObjectIdResponse(id=1821403, encodeId=45121821403ac, content=<a href='/topic/show?id=428a101612e7' target=_blank style='color:#2F92EE;'>#骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=69, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101612, encryptionId=428a101612e7, topicName=骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/qXbLjJB4FSzhQS3C5qQFMJkqicRIJFL6zHFwwhv0I95nzA6xicevQcEica0gk4A58UkbMMriadAgTY2b7N7Yd0ZsQg/132, createdBy=910c2500152, createdName=HinsMax, createdTime=Fri Jun 21 20:32:00 CST 2019, time=2019-06-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1815469, encodeId=26dc181546920, content=<a href='/topic/show?id=ae359e231d4' target=_blank style='color:#2F92EE;'>#间充质干细#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=62, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=97231, encryptionId=ae359e231d4, topicName=间充质干细)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4b632500090, createdName=h.y200607_23674418, createdTime=Sat Jan 04 03:32:00 CST 2020, time=2020-01-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1878302, encodeId=01cf18e8302d1, content=<a href='/topic/show?id=c157696175' target=_blank style='color:#2F92EE;'>#ERS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6961, encryptionId=c157696175, topicName=ERS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e93e105, createdName=anminleiryan, createdTime=Sat Dec 21 14:32:00 CST 2019, time=2019-12-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1989650, encodeId=c50c1989650ba, content=<a href='/topic/show?id=541c239345a' target=_blank style='color:#2F92EE;'>#二极管#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=131, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=23934, encryptionId=541c239345a, topicName=二极管)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e183245, createdName=Eleven11, createdTime=Sat Aug 10 00:32:00 CST 2019, time=2019-08-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1797991, encodeId=68571e9799173, content=<a href='/topic/show?id=43de6e74810' target=_blank style='color:#2F92EE;'>#牙龈间充质干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67748, encryptionId=43de6e74810, topicName=牙龈间充质干细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1e1c493, createdName=xugc, createdTime=Sun Nov 17 17:32:00 CST 2019, time=2019-11-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337545, encodeId=f615133e5450e, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Wed Mar 06 10:32:00 CST 2019, time=2019-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352046, encodeId=ffa21352046fe, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Wed Mar 06 10:32:00 CST 2019, time=2019-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609620, encodeId=77f6160962069, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Wed Mar 06 10:32:00 CST 2019, time=2019-03-06, status=1, ipAttribution=)]
    2019-03-06 zhaojie88
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