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INT J LAB HEMATOL:通过流式细胞术评估骨髓增生异常的参考范围

2019-02-01 MedSci MedSci原创

目前,已经提出了基于四个参数的流式细胞术评分(FCS)用于分析具有<5%原始细胞的骨髓增生异常综合征患者。在此,研究人员评估了从股骨头分离的骨髓抽取样本是否可用于验证个体参数评分(IPS)的截止值,有助于FCS并比较两个中心的FCS参数的适用性。

研究人员从接受髋关节置换手术的患者的股骨头获得骨髓细胞。使用它们自己的设施和方法在两个中心独​​立地获得细胞样品的4个单独参数的得分。随后在另一个中心交换并重新分析流式细胞仪数据文件。比较得到的四个数据集,以评估两个中心的重现性和结果。

结果显示,40个骨髓样本中有29个含有足够的细胞用于分析。4个单独参数中的3个的建议截止值是合适的。所有29个样品在4个获得的数据集中的1个中显示阳性个体参数评分(IPS1)。PS评分的大部分差异是由于重新分析了另一个中心的数据文件,而不是数据采集的结果。FCS:在11个样品中观察到≥2

研究表明,来自股骨头的骨髓样品适用于验证各个参数的建议参考截止值。需要对建议的参考截止值进行调整,以便进行可靠的解释。数据采集​​和数据分析的标准化对于获得统一的结果是必要的。

原始出处:

Adriaan J. van Gammeren M. van Rijckevorsel Anne E. Bras. Usability of femoral head bone marrow to verify reference ranges for the assessment of myelodysplasia by flow cytometry

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    2019-02-03 衣带渐宽

    学习

    0

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    2019-02-03 fengyi812
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