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Tissue Eng Part A：纳米图案化的PEG-GelMA-HA水凝胶增强牙髓干细胞的软骨形成分化

2019-05-20 不详 网络

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我们已经研究了表面纳米形貌和透明质酸（HA）对牙髓干细胞（DPSC）的体外软骨形成的影响。采用紫外辅助毛细管力光刻法制备由聚（乙二醇）二甲基丙烯酸酯（PEGDMA），甲基丙烯酸酯化明胶（GelMA）和HA组成的复合PEG-GelMA-HA水凝胶的明确定义的纳米结构支架。本研究首先证明了DPSCs形成了三维球状体，为体外软骨细胞分化提供了合适的环境。此外，研究还发现，与组织培养聚苯乙烯培养的DPSC

我们已经研究了表面纳米形貌和透明质酸（HA）对牙髓干细胞（DPSC）的体外软骨形成的影响。

采用紫外辅助毛细管力光刻法制备由聚（乙二醇）二甲基丙烯酸酯（PEGDMA），甲基丙烯酸酯化明胶（GelMA）和HA组成的复合PEG-GelMA-HA水凝胶的明确定义的纳米结构支架。

本研究首先证明了DPSCs形成了三维球状体，为体外软骨细胞分化提供了合适的环境。此外，研究还发现，与组织培养聚苯乙烯培养的DPSC相比，在纳米图案PEG-GelMA-HA支架上培养的DPSC显示出软骨形成基因标记物（Sox9，碱性磷酸酶，聚蛋白多糖，II型前胶原和X型前胶原）的明显上调，同时多能干细胞基因，Nanog和上皮-间充质基因（Twist，Snail，Slug）的表达下调。免疫细胞化学显示在纳米图案化的PEG-GelMA-HA支架上培养的DPSC中更广泛地沉积II型胶原。

总之，这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。

原始出处：

Nemeth CL1, Janebodin K, et al., Enhanced chondrogenic differentiation of dental pulp stem cells using nanopatterned PEG-GelMA-HA hydrogels. Tissue Eng Part A. 2014 Nov;20(21-22):2817-29. doi: 10.1089/ten.TEA.2013.0614. Epub 2014 Jun 30.

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    2019-09-23 jxrzshh

    #牙髓#

    0

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    2019-10-11 feather89

    #PE#

    0

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    2020-03-25 xuyu

    #Tissue#

    0

  4. 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createdBy=f06c33759375, createdName=feather89, createdTime=Fri Oct 11 20:13:00 CST 2019, time=2019-10-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911382, encodeId=85ba1911382b2, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Wed Mar 25 18:13:00 CST 2020, time=2020-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2081559, encodeId=6d42208155999, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Mon Jan 27 21:13:00 CST 2020, time=2020-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=367016, encodeId=209d36e0161b, content=发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 30 21:33:49 CST 2019, time=2019-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366682, encodeId=1ca1366682ea, content=这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Fri May 24 07:07:22 CST 2019, time=2019-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366666, encodeId=ba123666663f, content=表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 23 21:34:26 CST 2019, time=2019-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308055, encodeId=9541130805599, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357192, encodeId=e374135e192af, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1461026, encodeId=fce7146102621, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=)]
    2020-01-27 spoonycyy

    #Eng#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1669773, encodeId=7ca71669e734f, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Mon Sep 23 12:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727990, encodeId=60ec1e27990f8, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Fri Oct 11 20:13:00 CST 2019, time=2019-10-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911382, encodeId=85ba1911382b2, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Wed Mar 25 18:13:00 CST 2020, time=2020-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2081559, encodeId=6d42208155999, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Mon Jan 27 21:13:00 CST 2020, time=2020-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=367016, encodeId=209d36e0161b, content=发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 30 21:33:49 CST 2019, time=2019-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366682, encodeId=1ca1366682ea, content=这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Fri May 24 07:07:22 CST 2019, time=2019-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366666, encodeId=ba123666663f, content=表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 23 21:34:26 CST 2019, time=2019-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308055, encodeId=9541130805599, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357192, encodeId=e374135e192af, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1461026, encodeId=fce7146102621, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=)]
    2019-05-30 zyh4018

    发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1669773, encodeId=7ca71669e734f, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Mon Sep 23 12:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727990, encodeId=60ec1e27990f8, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Fri Oct 11 20:13:00 CST 2019, time=2019-10-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911382, encodeId=85ba1911382b2, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Wed Mar 25 18:13:00 CST 2020, time=2020-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2081559, encodeId=6d42208155999, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Mon Jan 27 21:13:00 CST 2020, time=2020-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=367016, encodeId=209d36e0161b, content=发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 30 21:33:49 CST 2019, time=2019-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366682, encodeId=1ca1366682ea, content=这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Fri May 24 07:07:22 CST 2019, time=2019-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366666, encodeId=ba123666663f, content=表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 23 21:34:26 CST 2019, time=2019-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308055, encodeId=9541130805599, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357192, encodeId=e374135e192af, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1461026, encodeId=fce7146102621, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=)]
    2019-05-24 zyh4018

    这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1669773, encodeId=7ca71669e734f, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Mon Sep 23 12:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727990, encodeId=60ec1e27990f8, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Fri Oct 11 20:13:00 CST 2019, time=2019-10-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911382, encodeId=85ba1911382b2, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Wed Mar 25 18:13:00 CST 2020, time=2020-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2081559, encodeId=6d42208155999, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Mon Jan 27 21:13:00 CST 2020, time=2020-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=367016, encodeId=209d36e0161b, content=发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 30 21:33:49 CST 2019, time=2019-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366682, encodeId=1ca1366682ea, content=这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Fri May 24 07:07:22 CST 2019, time=2019-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366666, encodeId=ba123666663f, content=表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 23 21:34:26 CST 2019, time=2019-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308055, encodeId=9541130805599, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357192, encodeId=e374135e192af, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1461026, encodeId=fce7146102621, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=)]
    2019-05-23 zyh4018

    表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=1669773, encodeId=7ca71669e734f, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Mon Sep 23 12:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727990, encodeId=60ec1e27990f8, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Fri Oct 11 20:13:00 CST 2019, time=2019-10-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911382, encodeId=85ba1911382b2, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Wed Mar 25 18:13:00 CST 2020, time=2020-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2081559, encodeId=6d42208155999, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Mon Jan 27 21:13:00 CST 2020, time=2020-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=367016, encodeId=209d36e0161b, content=发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 30 21:33:49 CST 2019, time=2019-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366682, encodeId=1ca1366682ea, content=这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Fri May 24 07:07:22 CST 2019, time=2019-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366666, encodeId=ba123666663f, content=表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 23 21:34:26 CST 2019, time=2019-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308055, encodeId=9541130805599, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357192, encodeId=e374135e192af, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1461026, encodeId=fce7146102621, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=)]
    2019-05-21 tastas

    #水凝胶#

    0

  9. [GetPortalCommentsPageByObjectIdResponse(id=1669773, encodeId=7ca71669e734f, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Mon Sep 23 12:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727990, encodeId=60ec1e27990f8, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Fri Oct 11 20:13:00 CST 2019, time=2019-10-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911382, encodeId=85ba1911382b2, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Wed Mar 25 18:13:00 CST 2020, time=2020-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2081559, encodeId=6d42208155999, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Mon Jan 27 21:13:00 CST 2020, time=2020-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=367016, encodeId=209d36e0161b, content=发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 30 21:33:49 CST 2019, time=2019-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366682, encodeId=1ca1366682ea, content=这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Fri May 24 07:07:22 CST 2019, time=2019-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366666, encodeId=ba123666663f, content=表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 23 21:34:26 CST 2019, time=2019-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308055, encodeId=9541130805599, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357192, encodeId=e374135e192af, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1461026, encodeId=fce7146102621, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=)]
    2019-05-21 zhaojie88

    #ART#

    0

  10. [GetPortalCommentsPageByObjectIdResponse(id=1669773, encodeId=7ca71669e734f, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Mon Sep 23 12:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727990, encodeId=60ec1e27990f8, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Fri Oct 11 20:13:00 CST 2019, time=2019-10-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911382, encodeId=85ba1911382b2, content=<a href='/topic/show?id=df471e4882e' target=_blank style='color:#2F92EE;'>#Tissue#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17488, encryptionId=df471e4882e, topicName=Tissue)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Wed Mar 25 18:13:00 CST 2020, time=2020-03-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2081559, encodeId=6d42208155999, content=<a href='/topic/show?id=57406e8506' target=_blank style='color:#2F92EE;'>#Eng#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6785, encryptionId=57406e8506, topicName=Eng)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3516494, createdName=spoonycyy, createdTime=Mon Jan 27 21:13:00 CST 2020, time=2020-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=367016, encodeId=209d36e0161b, content=发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 30 21:33:49 CST 2019, time=2019-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366682, encodeId=1ca1366682ea, content=这些发现表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Fri May 24 07:07:22 CST 2019, time=2019-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=366666, encodeId=ba123666663f, content=表明纳米形貌和HA为促进DPSC的软骨形成分化提供了重要的线索。, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/10/28/3628e0b9a9377f351a54894178b67039.jpg, createdBy=442b1338925, createdName=zyh4018, createdTime=Thu May 23 21:34:26 CST 2019, time=2019-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1308055, encodeId=9541130805599, content=<a href='/topic/show?id=edd364149bc' target=_blank style='color:#2F92EE;'>#水凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64149, encryptionId=edd364149bc, topicName=水凝胶)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=47c5349, createdName=tastas, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1357192, encodeId=e374135e192af, content=<a href='/topic/show?id=b0702e84b9' target=_blank style='color:#2F92EE;'>#ART#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2784, encryptionId=b0702e84b9, topicName=ART)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1461026, encodeId=fce7146102621, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue May 21 17:13:00 CST 2019, time=2019-05-21, status=1, ipAttribution=)]
    2019-05-21 lishizhe

    #凝胶#

    0

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