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Infect Genet Evol:寨卡病毒通过负面影响颅神经嵴发育诱导异常颅骨成骨

2019-01-25 MedSci MedSci原创

妊娠期间寨卡病毒(ZIKV)感染被认为通过神经发生期间神经系统的直接损伤与出生缺陷相关。然而,在这项研究中,我们的数据显示ZIKV感染显著抑制了鸡胚胎中的Safranin O / Fast Green和茜素红染色显示的颅骨成骨,这提供了由ZIKV引起的颅面骨畸形的另一种可能性。通过免疫荧光染色和电子显微镜,我们证实了鸡胚神经管和神经嵴部位的ZIKV感染。接下来,体内(鸡胚)和体外[神经嵴细胞(NC

妊娠期间寨卡病毒(ZIKV)感染被认为通过神经发生期间神经系统的直接损伤与出生缺陷相关。然而,在这项研究中,我们的数据显示ZIKV感染显著抑制了鸡胚胎中的Safranin O / Fast Green和茜素红染色显示的颅骨成骨,这提供了由ZIKV引起的颅面骨畸形的另一种可能性。

通过免疫荧光染色和电子显微镜,我们证实了鸡胚神经管和神经嵴部位的ZIKV感染。接下来,体内(鸡胚)和体外[神经嵴细胞(NCC)的原代培养物] ZIKV和HNK-1双重免疫荧光染色证明ZIKV感染抑制迁移性NCC的产生。由ZIKV感染的HH10鸡胚中AP-2α-和Pax7-阳性NCC的减少证实,在迁移过程中也发生了颅NCC的异常发育。整体原位杂交证明,在ZIKV感染的HH10鸡胚中,钙粘蛋白6B表达升高并且Slug,FoxD3和BMP4 / Msx1表达降低,这意味着神经嵴产生的上皮-间质转化(EMT)被ZIKV感染阻断。此外,体内和体外pHIS3和Pax7双重免疫荧光染色显示ZCCV感染抑制NCC增殖。HH10鸡胚胎中C-caspase-3和AP-2α双重免疫荧光染色和Western印迹显示ZIKV感染后NCC凋亡增加。最后,电子显微镜显示在ZIKV感染的胚胎中有多个自噬体,Western印迹和LC3B免疫荧光染色证明自噬相关基因被ZIKV感染激活。

总之,我们的数据首次表明,胚胎发生过程中的ZIKV感染可能干扰颅神经嵴发育,进而导致颅骨成骨异常。我们的结果为ZIKV感染引起的脑畸形提供了新的见解。

原始出处:

Yan Y1, Zhang XT, et al. Zika virus induces abnormal cranial osteogenesis by negatively affecting cranial neural crest development. Infect Genet Evol. 2019 Jan 18. pii: S1567-1348(18)30781-0. doi: 10.1016/j.meegid.2019.01.023.

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    2019-06-01 zhaojie88
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    2019-10-13 智慧医人
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    2019-01-27 neurowu
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    2019-01-27 zhaojie88
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  8. [GetPortalCommentsPageByObjectIdResponse(id=1686271, encodeId=a56916862e18c, content=<a href='/topic/show?id=66f3e93862' target=_blank style='color:#2F92EE;'>#Genet#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7938, encryptionId=66f3e93862, topicName=Genet)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d3e128296421, createdName=江川靖瑶, createdTime=Mon Jun 24 03:33:00 CST 2019, time=2019-06-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2049135, encodeId=335c2049135ab, content=<a href='/topic/show?id=4363e929ad' target=_blank style='color:#2F92EE;'>#Gene#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7929, encryptionId=4363e929ad, topicName=Gene)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Sat Jun 01 18:33:00 CST 2019, time=2019-06-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1734104, encodeId=c84b1e3410481, content=<a href='/topic/show?id=d4f412668a4' target=_blank style='color:#2F92EE;'>#NET#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12668, encryptionId=d4f412668a4, topicName=NET)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=智慧医人, createdTime=Sun Oct 13 21:33:00 CST 2019, time=2019-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2023371, encodeId=a44720233e185, content=<a href='/topic/show?id=0e5f92283c8' target=_blank style='color:#2F92EE;'>#负面影响#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92283, encryptionId=0e5f92283c8, topicName=负面影响)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=80a244, createdName=d830372, createdTime=Thu Feb 21 09:33:00 CST 2019, time=2019-02-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1347368, encodeId=1554134e36836, content=<a href='/topic/show?id=88713e938c1' target=_blank style='color:#2F92EE;'>#发育#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37938, encryptionId=88713e938c1, topicName=发育)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f4c124, createdName=neurowu, createdTime=Sun Jan 27 14:33:00 CST 2019, time=2019-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351972, encodeId=f23213519e267, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Sun Jan 27 14:33:00 CST 2019, time=2019-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1360108, encodeId=07511360108ff, content=<a href='/topic/show?id=6783466083f' target=_blank style='color:#2F92EE;'>#寨卡病毒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=46608, encryptionId=6783466083f, topicName=寨卡病毒)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=583c133, createdName=yuandd, createdTime=Sun Jan 27 14:33:00 CST 2019, time=2019-01-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1512959, encodeId=f06f15129599e, content=<a href='/topic/show?id=f0364660360' target=_blank style='color:#2F92EE;'>#寨卡#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=46603, encryptionId=f0364660360, topicName=寨卡)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d79610514526, createdName=12498710m14暂无昵称, createdTime=Sun Jan 27 14:33:00 CST 2019, time=2019-01-27, status=1, ipAttribution=)]
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