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CLIN CANCER RES:肺癌瘤内注射CCL21基因修饰的DC细胞可以诱导肿瘤特异性免疫反应及CD8+T细胞浸润

2017-08-23 MedSci MedSci原创

CLIN CANCER RES近期发表一篇文章,评估了瘤内局部注射肿瘤疫苗的安全性及肿瘤抗原特异性免疫反应。晚期非小细胞肺癌(NSCLC)患者接受瘤内注射转载了表达CCL21基因腺病毒载体的自体DC细胞(Ad-CCL21-DC)。

CLIN CANCER RES近期发表一篇文章,评估了瘤内局部注射肿瘤疫苗的安全性及肿瘤抗原特异性免疫反应。晚期非小细胞肺癌(NSCLC)患者接受瘤内注射转载了表达CCL21基因腺病毒载体的自体DC细胞(Ad-CCL21-DC)。研究共纳入16例ⅢB/Ⅳ期的非小细胞肺癌患者,患者第0天和第7天经过CT或支气管镜引导瘤内注射DC细胞(1×106,5×106,1×107或3×107 DC/次)。通过ELISPOT分析肿瘤抗原特异性外周血淋巴细胞评估免疫反应。肿瘤组织活检,利用免疫组化评估CD8+T细胞以及利用免疫组化和rt-PCR评估PD-L1表达。研究结果表明,25%的患者在56天病情稳定。平均生存时间为3.9个月。ELISPOT分析表明6例患者出现针对肿瘤相关抗原(TAA)的系统性反应。54%的患者出现肿瘤CD8+T细胞浸润。肿瘤疫苗注射后CD8+细胞增加的患者出现PD-L1MRNA表达增高。文章最后认为,Ad-CCL21-DC瘤内疫苗会诱导系统性的肿瘤抗原特异性免疫反应,增加肿瘤CD8+T细胞浸润以及增加肿瘤PD-L1表达。需要进一步研究DC-CCL21瘤内疫苗与PD-1/PD-L1

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time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1599934, encodeId=1381159993404, content=<a href='/topic/show?id=7a7d4168e3a' target=_blank style='color:#2F92EE;'>#基因修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=41687, encryptionId=7a7d4168e3a, topicName=基因修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=069c18767911, createdName=apoenzyme, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=)]
    2018-02-18 1e145228m78(暂无匿称)

    学习了.谢谢作者分享!

    0

  3. 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time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1599934, encodeId=1381159993404, content=<a href='/topic/show?id=7a7d4168e3a' target=_blank style='color:#2F92EE;'>#基因修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=41687, encryptionId=7a7d4168e3a, topicName=基因修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=069c18767911, createdName=apoenzyme, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=)]
    2018-02-16 1e145228m78(暂无匿称)

    学习了.谢谢作者分享!

    0

  4. 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createdName=1e145228m78(暂无匿称), createdTime=Sun Feb 18 18:08:24 CST 2018, time=2018-02-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=288385, encodeId=9f3028838578, content=学习了.谢谢作者分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=134, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3xJXLtMtt5B40XXuPjn9xn1rBhrM6fHLkX1hI86ZoWoPJkgDkZuJNKkBfBHOcRd1fAdezg7JlHNOokohdAp3WkI/0, createdBy=32c81964262, createdName=1e145228m78(暂无匿称), createdTime=Fri Feb 16 11:11:29 CST 2018, time=2018-02-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1784044, encodeId=99d21e8404455, content=<a href='/topic/show?id=4b5c564252' target=_blank style='color:#2F92EE;'>#D8+T#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5642, encryptionId=4b5c564252, topicName=D8+T)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f612331, createdName=gds2009, createdTime=Thu Oct 05 20:21:00 CST 2017, time=2017-10-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1899153, encodeId=d48c1899153c8, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=118, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Tue Jul 31 03:21:00 CST 2018, time=2018-07-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=236923, encodeId=21f2236923ea, content=好文.值得点赞!认真学习了.把经验应用于实践.为患者解除病痛., beContent=null, objectType=article, channel=null, level=null, likeNumber=143, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Fri Aug 25 08:12:09 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1280167, encodeId=33d5128016e56, content=<a href='/topic/show?id=2bbe289846f' target=_blank style='color:#2F92EE;'>#免疫反应#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=28984, encryptionId=2bbe289846f, topicName=免疫反应)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=827b160, createdName=cnxcy, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1534044, encodeId=3af11534044d2, content=<a href='/topic/show?id=6ea85e6117' target=_blank style='color:#2F92EE;'>#DC细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5761, encryptionId=6ea85e6117, topicName=DC细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=067412523287, createdName=zhangyxzsh, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1569295, encodeId=b3851569295c5, content=<a href='/topic/show?id=1c562e42646' target=_blank style='color:#2F92EE;'>#修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27426, encryptionId=1c562e42646, topicName=修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=29c615505859, createdName=zhangyxzsh, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1599934, encodeId=1381159993404, content=<a href='/topic/show?id=7a7d4168e3a' target=_blank style='color:#2F92EE;'>#基因修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=41687, encryptionId=7a7d4168e3a, topicName=基因修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=069c18767911, createdName=apoenzyme, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=)]
    2017-10-05 gds2009
  5. 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time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1599934, encodeId=1381159993404, content=<a href='/topic/show?id=7a7d4168e3a' target=_blank style='color:#2F92EE;'>#基因修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=41687, encryptionId=7a7d4168e3a, topicName=基因修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=069c18767911, createdName=apoenzyme, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=)]
  6. 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encryptionId=4b5c564252, topicName=D8+T)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f612331, createdName=gds2009, createdTime=Thu Oct 05 20:21:00 CST 2017, time=2017-10-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1899153, encodeId=d48c1899153c8, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=118, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Tue Jul 31 03:21:00 CST 2018, time=2018-07-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=236923, encodeId=21f2236923ea, content=好文.值得点赞!认真学习了.把经验应用于实践.为患者解除病痛., beContent=null, objectType=article, channel=null, level=null, likeNumber=143, replyNumber=0, 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href='/topic/show?id=6ea85e6117' target=_blank style='color:#2F92EE;'>#DC细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5761, encryptionId=6ea85e6117, topicName=DC细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=067412523287, createdName=zhangyxzsh, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1569295, encodeId=b3851569295c5, content=<a href='/topic/show?id=1c562e42646' target=_blank style='color:#2F92EE;'>#修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27426, encryptionId=1c562e42646, topicName=修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=29c615505859, createdName=zhangyxzsh, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1599934, encodeId=1381159993404, content=<a href='/topic/show?id=7a7d4168e3a' target=_blank style='color:#2F92EE;'>#基因修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=41687, encryptionId=7a7d4168e3a, topicName=基因修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=069c18767911, createdName=apoenzyme, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=)]
    2017-08-25 ylzr123

    好文.值得点赞!认真学习了.把经验应用于实践.为患者解除病痛.

    0

  7. 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  8. 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  9. 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href='/topic/show?id=6ea85e6117' target=_blank style='color:#2F92EE;'>#DC细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5761, encryptionId=6ea85e6117, topicName=DC细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=067412523287, createdName=zhangyxzsh, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1569295, encodeId=b3851569295c5, content=<a href='/topic/show?id=1c562e42646' target=_blank style='color:#2F92EE;'>#修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27426, encryptionId=1c562e42646, topicName=修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=29c615505859, createdName=zhangyxzsh, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1599934, encodeId=1381159993404, content=<a href='/topic/show?id=7a7d4168e3a' target=_blank style='color:#2F92EE;'>#基因修饰#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=41687, encryptionId=7a7d4168e3a, topicName=基因修饰)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=069c18767911, createdName=apoenzyme, createdTime=Fri Aug 25 05:21:00 CST 2017, time=2017-08-25, status=1, ipAttribution=)]
    2017-08-25 zhangyxzsh
  10. 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