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JCLA:如何准确测定25-羟基维生素D浓度?

2017-05-29 MedSci MedSci原创

近年来,关于维生素D的人口研究急剧增加。这些研究通常依赖于对25-羟基维生素D [25(OH)D]浓度的测定。

近日,国际杂志Journal of Clinical Laboratory Analysis上在线发表一项关于LC-MS / MSDiasorin RIA在大量人口调查中测定25-羟基维生素D浓度的研究。

近年来,关于维生素D的人口研究急剧增加。这些研究通常依赖于对25-羟基维生素D [25OHD]浓度的测定。

使用LC-MS / MS在北芬兰出生(1966年)队列的5,915名参与者(31岁)中测量25OHD325OHD2浓度。在18个月的时间内对血液样品分批测定。由于测量中存出现异常值,使用Diasorin RIA重新测定了200个样品。测量之间的一致性通过Passing-Bablok回归和协议限制(LoA)进行评估。

研究发现通过LC-MS / MS测量的浓度远高于Diasorin RIA测定的浓度,平均差异为12.9ng / ml。在对数转化后的批量测量值之间,存在稳定的明显的差异。对于每批LC-MS / MS测量分别应用统计公式,能够在转化前清除明显的稳定和比例误差。

研究认为尽管引入了方案及程序来提高测定25OHD的测定的准确性,但仍然可能发生显著的差异。在这些情况下,可以通过用基于相对较少数量的重复来统一测量的方法,并建立置信度,使研究间能够进行比较。

原始出处:

Diane J. Berry, John Dutton, William D, et.al. Harmonization Study Between LC-MS/MS and Diasorin RIA for Measurement of 25-Hydroxyvitamin D Concentrations in a Large Population Survey.

本文系梅斯医学(MedSci)原创编译整理,转载需授权!


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    2017-06-11 189****7206

    学习了谢谢分享。

    0

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    2017-06-09 130****4638

    学习了谢谢分享。

    0

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createdName=189****7206, createdTime=Fri Jun 02 00:18:26 CST 2017, time=2017-06-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=205098, encodeId=7b1420509888, content=文章很好,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Thu Jun 01 06:55:26 CST 2017, time=2017-06-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204963, encodeId=1a8b204963c5, content=不错的文章,学习到很有意思的东西, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Wed May 31 23:07:22 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544443, encodeId=0a58154444307, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Wed May 31 14:40:00 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204721, encodeId=dac6204e21ff, content=文章不错,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Wed May 31 08:15:35 CST 2017, time=2017-05-31, status=1, ipAttribution=)]
    2017-06-07 130****4638

    学习了谢谢分享。

    0

  5. 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createdName=189****7206, createdTime=Fri Jun 02 00:18:26 CST 2017, time=2017-06-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=205098, encodeId=7b1420509888, content=文章很好,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Thu Jun 01 06:55:26 CST 2017, time=2017-06-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204963, encodeId=1a8b204963c5, content=不错的文章,学习到很有意思的东西, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Wed May 31 23:07:22 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544443, encodeId=0a58154444307, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Wed May 31 14:40:00 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204721, encodeId=dac6204e21ff, content=文章不错,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Wed May 31 08:15:35 CST 2017, time=2017-05-31, status=1, ipAttribution=)]
    2017-06-05 184****9840

    学习了谢谢分享。

    0

  6. 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createdName=131****2916, createdTime=Wed May 31 23:07:22 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544443, encodeId=0a58154444307, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Wed May 31 14:40:00 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204721, encodeId=dac6204e21ff, content=文章不错,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Wed May 31 08:15:35 CST 2017, time=2017-05-31, status=1, ipAttribution=)]
    2017-06-02 189****7206

    学习了谢谢分享。

    0

  7. 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createdName=189****7206, createdTime=Fri Jun 02 00:18:26 CST 2017, time=2017-06-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=205098, encodeId=7b1420509888, content=文章很好,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Thu Jun 01 06:55:26 CST 2017, time=2017-06-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204963, encodeId=1a8b204963c5, content=不错的文章,学习到很有意思的东西, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Wed May 31 23:07:22 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544443, encodeId=0a58154444307, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Wed May 31 14:40:00 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204721, encodeId=dac6204e21ff, content=文章不错,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Wed May 31 08:15:35 CST 2017, time=2017-05-31, status=1, ipAttribution=)]
    2017-06-01 1e10c84am36(暂无匿称)

    文章很好,拜读了

    0

  8. 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createdName=189****7206, createdTime=Fri Jun 02 00:18:26 CST 2017, time=2017-06-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=205098, encodeId=7b1420509888, content=文章很好,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Thu Jun 01 06:55:26 CST 2017, time=2017-06-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204963, encodeId=1a8b204963c5, content=不错的文章,学习到很有意思的东西, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Wed May 31 23:07:22 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544443, encodeId=0a58154444307, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Wed May 31 14:40:00 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204721, encodeId=dac6204e21ff, content=文章不错,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Wed May 31 08:15:35 CST 2017, time=2017-05-31, status=1, ipAttribution=)]
    2017-05-31 131****2916

    不错的文章,学习到很有意思的东西

    0

  9. 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createdName=131****2916, createdTime=Wed May 31 23:07:22 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544443, encodeId=0a58154444307, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Wed May 31 14:40:00 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204721, encodeId=dac6204e21ff, content=文章不错,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Wed May 31 08:15:35 CST 2017, time=2017-05-31, status=1, ipAttribution=)]
  10. 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createdName=189****7206, createdTime=Fri Jun 02 00:18:26 CST 2017, time=2017-06-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=205098, encodeId=7b1420509888, content=文章很好,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Thu Jun 01 06:55:26 CST 2017, time=2017-06-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204963, encodeId=1a8b204963c5, content=不错的文章,学习到很有意思的东西, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Wed May 31 23:07:22 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544443, encodeId=0a58154444307, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Wed May 31 14:40:00 CST 2017, time=2017-05-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=204721, encodeId=dac6204e21ff, content=文章不错,拜读了, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Wed May 31 08:15:35 CST 2017, time=2017-05-31, status=1, ipAttribution=)]
    2017-05-31 1e10c84am36(暂无匿称)

    文章不错,拜读了

    0

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在《过敏和临床免疫学杂志》上发表的一项新研究发现,在怀孕期间服用维生素D补充剂可以积极地改变新生儿的免疫系统,这可以有助于新生儿防止哮喘和呼吸道感染。英国伦敦国王学院的研究人员研究了在怀孕的第二和第三个月每天补充4,400 IU维生素D3对新生儿的影响情况。

维生素D预防癌症的真相

    我们都知道维生素D可以帮助钙的吸收、促进骨骼健康,也能增强免疫力,最近的一些研究甚至说它还可以预防老年痴呆症、结肠癌和乳腺癌。但这种关联到底有多强呢?最近发表在著名医学期刊《Journal of the American Medical Association》(简称JAMA)上的一篇文章也许可以回答这个问题。 这项历时4年的研究将2300名绝经后女性随机分成

J Endocrinol:维生素D或可成为止痛剂、安眠药

最新研究表明,维生素D补充与良好睡眠习惯相结合可能有助于治疗与疼痛相关的大量疾病。该研究对应文章发表于最新出版的Journal of Endocrinology杂志,名为The interfaces between vitamin D, sleep and pain。指出人类维生素D摄入与吸收水平与优质的睡眠质量可以帮助包括关节炎,月经痉挛和慢性背痛等疾病的治疗。

ASCO2017:维生素D也是宝!高剂量维生素D或延缓转移性结直肠癌进展

一项由Dana-Farber癌症研究所研究人员开展的一项多机构的维生素D随机、单盲治疗癌症患者临床试验发现,高剂量微生物或可减缓转移性结直肠癌患者的疾病进展,相关研究结果发表在6月2-6日在美国芝加哥举行的第53届美国临床肿瘤学会年会(ASCO)上。

JAHA:钙补充剂有或无维生素D与缺血性卒中相关风险分析!

这项研究表明钙补充剂作为单药治疗且较高剂量时可能会增加缺血性卒中的风险,而他们与维生素D的组合似乎抵消了这种风险。

J Endocrinol:补充维生素D + 睡个好觉 疼痛缓解

继一项关于维生素D、睡眠与疼痛之间相互关系的研究发表之后,研究人员认为,补充维生素D,再加上良好的睡眠健康状况,可有效地缓解因关节炎、慢性背部疼痛、纤维肌痛和痛经所致的疼痛。

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