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Curr Mol Med：细胞松弛素D可用于颅颌面缺损的骨修复

2019-10-13 不详 网络

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由创伤，肿瘤切除，感染或先天性畸形引起的骨缺损是一种常见的临床疾病。骨组织工程被认为是骨缺损重建中的一种有前途的方法。因此，可以促进成骨作用的药物受到极大关注。细胞松弛素D（Cyto D）是一种来自霉菌的代谢产物，被证明能够修饰肌动蛋白并重组细胞骨架。本研究旨在探讨Cyto D对小鼠成骨细胞MC3T3-E1细胞成骨分化的作用及其机制。通过ALP染色，ARS，Western印迹和RT-PCR评估了C

由创伤，肿瘤切除，感染或先天性畸形引起的骨缺损是一种常见的临床疾病。骨组织工程被认为是骨缺损重建中的一种有前途的方法。因此，可以促进成骨作用的药物受到极大关注。细胞松弛素D（Cyto D）是一种来自霉菌的代谢产物，被证明能够修饰肌动蛋白并重组细胞骨架。本研究旨在探讨Cyto D对小鼠成骨细胞MC3T3-E1细胞成骨分化的作用及其机制。

通过ALP染色，ARS，Western印迹和RT-PCR评估了Cyto D诱导的MC3T3-E1细胞的成骨分化，并利用特异性途径抑制剂来探讨MAPK途径是否参与了这一过程。

结果表明，最佳作用浓度为10-2μg/ml。Cyto D的添加可以显着上调Runx2，OCN和OSX的表达，增强ALP活性，改善钙沉积，并促进p38蛋白的磷酸化水平。该途径的抑制明显降低了p38的激活以及成骨相关基因的表达。

总之，该研究结果表明，Cyto D可以通过p38-MAPK信号通路促进MC3T3细胞的成骨分化，但不能促进ERK1/2或JNK的成骨分化，它是改善成骨性的潜在药物。

原始出处：

Liu Q, Zhuang Y, et al., Cytochalasin D Promotes Osteogenic Differentiation of MC3T3-E1 Cells via p38-MAPK Signaling Pathway. Curr Mol Med. 2019 Oct 6. doi: 10.2174/1566524019666191007104816. 

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    2020-01-04 宋威

    #颅颌面#

    0

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    2020-05-07 feifers

    #细胞松弛素#

    0

  3. 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2020-01-11 axin020

    #骨修复#

    0

  4. 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2019-10-16 yjs木玉

    好好好好好好

    0

  5. 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2019-10-15 ms3994565386320060

    #Med#

    0

  6. 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2019-10-15 1e1c07bdm01(暂无匿称)

    好

    0

  7. 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2019-10-14 1ddf0692m34(暂无匿称)

    学习了，长知识

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=1820278, encodeId=75c418202e8d5, content=<a href='/topic/show?id=f7fc1004806c' target=_blank style='color:#2F92EE;'>#颅颌面#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100480, encryptionId=f7fc1004806c, topicName=颅颌面)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6105386, createdName=宋威, createdTime=Sat Jan 04 19:53:00 CST 2020, time=2020-01-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1805347, encodeId=9ffa180534ee4, content=<a href='/topic/show?id=cfe1e79231c' target=_blank style='color:#2F92EE;'>#细胞松弛素#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77923, encryptionId=cfe1e79231c, topicName=细胞松弛素)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1f1d390, createdName=feifers, createdTime=Thu May 07 15:53:00 CST 2020, time=2020-05-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1719503, encodeId=e5781e19503d3, content=<a href='/topic/show?id=17641015914f' target=_blank style='color:#2F92EE;'>#骨修复#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=77, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101591, encryptionId=17641015914f, topicName=骨修复)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7a5232746261, createdName=axin020, createdTime=Sat Jan 11 20:53:00 CST 2020, time=2020-01-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373975, encodeId=155e3e397599, content=好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Wed Oct 16 06:06:02 CST 2019, time=2019-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610029, encodeId=2ac91610029b4, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Oct 15 14:53:00 CST 2019, time=2019-10-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373932, encodeId=47403e39321f, content=好, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Tue Oct 15 04:14:38 CST 2019, time=2019-10-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373896, encodeId=f0123e3896ab, content=学习了，长知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Mon Oct 14 08:13:38 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373895, encodeId=72cc3e389513, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/08/03/0e492dda8bcc1f98474b74c2863aca87.jpg, createdBy=068d2446376, createdName=Dr Z, createdTime=Mon Oct 14 07:34:37 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373890, encodeId=e6fa3e38908f, content=学习学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRkia1DP5MpwEgtXo1XhXA11xD6MWKxunAZFXKSNic0fWHUQx83oNHPlRMxxIctM83QKeCLkJpMibibiaC/0, createdBy=7e581734408, createdName=深海的鱼, createdTime=Mon Oct 14 06:21:29 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373884, encodeId=ad643e388471, content=学习了，很有用，不错, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2019-10-14 Dr Z

    学习了

    0

  9. [GetPortalCommentsPageByObjectIdResponse(id=1820278, encodeId=75c418202e8d5, content=<a href='/topic/show?id=f7fc1004806c' target=_blank style='color:#2F92EE;'>#颅颌面#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100480, encryptionId=f7fc1004806c, topicName=颅颌面)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6105386, createdName=宋威, createdTime=Sat Jan 04 19:53:00 CST 2020, time=2020-01-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1805347, encodeId=9ffa180534ee4, content=<a href='/topic/show?id=cfe1e79231c' target=_blank style='color:#2F92EE;'>#细胞松弛素#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77923, encryptionId=cfe1e79231c, topicName=细胞松弛素)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1f1d390, 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Tue Oct 15 04:14:38 CST 2019, time=2019-10-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373896, encodeId=f0123e3896ab, content=学习了，长知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Mon Oct 14 08:13:38 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373895, encodeId=72cc3e389513, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/08/03/0e492dda8bcc1f98474b74c2863aca87.jpg, createdBy=068d2446376, createdName=Dr Z, createdTime=Mon Oct 14 07:34:37 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373890, encodeId=e6fa3e38908f, content=学习学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRkia1DP5MpwEgtXo1XhXA11xD6MWKxunAZFXKSNic0fWHUQx83oNHPlRMxxIctM83QKeCLkJpMibibiaC/0, createdBy=7e581734408, createdName=深海的鱼, createdTime=Mon Oct 14 06:21:29 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373884, encodeId=ad643e388471, content=学习了，很有用，不错, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2019-10-14 深海的鱼

    学习学习学习

    0

  10. [GetPortalCommentsPageByObjectIdResponse(id=1820278, encodeId=75c418202e8d5, content=<a href='/topic/show?id=f7fc1004806c' target=_blank style='color:#2F92EE;'>#颅颌面#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100480, encryptionId=f7fc1004806c, topicName=颅颌面)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6105386, createdName=宋威, createdTime=Sat Jan 04 19:53:00 CST 2020, time=2020-01-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1805347, encodeId=9ffa180534ee4, content=<a href='/topic/show?id=cfe1e79231c' target=_blank style='color:#2F92EE;'>#细胞松弛素#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77923, encryptionId=cfe1e79231c, topicName=细胞松弛素)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1f1d390, 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Tue Oct 15 04:14:38 CST 2019, time=2019-10-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373896, encodeId=f0123e3896ab, content=学习了，长知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Mon Oct 14 08:13:38 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373895, encodeId=72cc3e389513, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/08/03/0e492dda8bcc1f98474b74c2863aca87.jpg, createdBy=068d2446376, createdName=Dr Z, createdTime=Mon Oct 14 07:34:37 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373890, encodeId=e6fa3e38908f, content=学习学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRkia1DP5MpwEgtXo1XhXA11xD6MWKxunAZFXKSNic0fWHUQx83oNHPlRMxxIctM83QKeCLkJpMibibiaC/0, createdBy=7e581734408, createdName=深海的鱼, createdTime=Mon Oct 14 06:21:29 CST 2019, time=2019-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=373884, encodeId=ad643e388471, content=学习了，很有用，不错, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Mon Oct 14 02:03:13 CST 2019, time=2019-10-14, status=1, ipAttribution=)]
    2019-10-14 飛歌

    学习了，很有用，不错

    0

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