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Clin Chem：低病毒载量热灭活新冠肺炎标本的潜在假阴性核酸检测结果

2020-07-19 MedSci原创 MedSci原创

假阴性；核酸；检测
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2019年底以来，2019冠状病毒病(COVID-19)在全球急速传播。核酸检测(NAT)在COVID-19患者诊断和治疗中发挥了重要作用。

2019年底以来，2019冠状病毒病(COVID-19)在全球急速传播。核酸检测(NAT)在COVID-19患者诊断和治疗中发挥了重要作用。在某些情况下，56℃的热灭活策略被推荐用于在NAT之前对SARS-CoV-2灭活。然而，理论上，这种方法可能会破坏这种单链RNA病毒的核酸完整性，并在实时聚合酶链反应(RT-PCR)测试中造成假阴性。

本研究了热灭活是否会影响病毒NAT的结果。研究人员检测了热灭活对SARS-CoV-2定量RT-PCR结果的影响，特别是对低病毒载量标本的假阴性结果的影响。此外，还研究了不同标本类型、标本保存时间和化学灭活方法对NAT的影响。

研究显示，对确诊的COVID-19患者进行RT-PCR检测后，热孵育期Ct值升高。此外，在至少一次平行检测中，约一半的弱阳性样本(15个样本中的7个，46.7%)经热灭活后RT-PCR阴性。使用胍基裂解法保存标本对RT-PCR结果的影响较小，假阴性率更低(15个标本中有2个，占13.3%)，Ct值的升高明显小于热灭活法。

研究结果表明，热灭活对RT-PCR检测SARS-CoV-2的效率有不利影响。鉴于化学灭活化剂的适用范围有限，需要考虑其他确保对实验室人员进行全面保护的方法。

原始出处：

Yang Pan, Luyao Long,Potential False-Negative Nucleic Acid Testing Results for Severe Acute Respiratory Syndrome Coronavirus 2 from Thermal Inactivation of Samples with Low Viral Loads

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    2020-09-01 ms306040998833292

    #标本#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1758983, encodeId=c3561e589831b, content=<a href='/topic/show?id=39e96195411' target=_blank style='color:#2F92EE;'>#标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61954, encryptionId=39e96195411, topicName=标本)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=45f437392611, createdName=ms306040998833292, createdTime=Tue Sep 01 12:50:10 CST 2020, time=2020-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2083712, encodeId=67b32083e120c, content=<a href='/topic/show?id=685862555f0' target=_blank style='color:#2F92EE;'>#检测结果#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62555, encryptionId=685862555f0, topicName=检测结果)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4dfa37, createdName=huhuaidong387, createdTime=Sun Mar 21 17:50:10 CST 2021, time=2021-03-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1491161, encodeId=d9ad1491161df, content=<a href='/topic/show?id=fb94e0991b3' target=_blank style='color:#2F92EE;'>#病毒载量#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=70991, encryptionId=fb94e0991b3, topicName=病毒载量)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=1c1e8876950, createdName=grace5703, createdTime=Tue Jul 21 06:50:10 CST 2020, time=2020-07-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1028311, encodeId=f82610283113e, content=新冠肺炎，疫情何时才能消失, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=公卫新人, createdTime=Sun Jul 19 18:50:10 CST 2020, time=2020-07-19, status=1, ipAttribution=)]
    2021-03-21 huhuaidong387

    #检测结果#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1758983, encodeId=c3561e589831b, content=<a href='/topic/show?id=39e96195411' target=_blank style='color:#2F92EE;'>#标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61954, encryptionId=39e96195411, topicName=标本)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=45f437392611, createdName=ms306040998833292, createdTime=Tue Sep 01 12:50:10 CST 2020, time=2020-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2083712, encodeId=67b32083e120c, content=<a href='/topic/show?id=685862555f0' target=_blank style='color:#2F92EE;'>#检测结果#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62555, encryptionId=685862555f0, topicName=检测结果)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4dfa37, createdName=huhuaidong387, createdTime=Sun Mar 21 17:50:10 CST 2021, time=2021-03-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1491161, encodeId=d9ad1491161df, content=<a href='/topic/show?id=fb94e0991b3' target=_blank style='color:#2F92EE;'>#病毒载量#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=70991, encryptionId=fb94e0991b3, topicName=病毒载量)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=1c1e8876950, createdName=grace5703, createdTime=Tue Jul 21 06:50:10 CST 2020, time=2020-07-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1028311, encodeId=f82610283113e, content=新冠肺炎，疫情何时才能消失, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=公卫新人, createdTime=Sun Jul 19 18:50:10 CST 2020, time=2020-07-19, status=1, ipAttribution=)]
    2020-07-21 grace5703

    #病毒载量#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1758983, encodeId=c3561e589831b, content=<a href='/topic/show?id=39e96195411' target=_blank style='color:#2F92EE;'>#标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61954, encryptionId=39e96195411, topicName=标本)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=45f437392611, createdName=ms306040998833292, createdTime=Tue Sep 01 12:50:10 CST 2020, time=2020-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2083712, encodeId=67b32083e120c, content=<a href='/topic/show?id=685862555f0' target=_blank style='color:#2F92EE;'>#检测结果#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62555, encryptionId=685862555f0, topicName=检测结果)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4dfa37, createdName=huhuaidong387, createdTime=Sun Mar 21 17:50:10 CST 2021, time=2021-03-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1491161, encodeId=d9ad1491161df, content=<a href='/topic/show?id=fb94e0991b3' target=_blank style='color:#2F92EE;'>#病毒载量#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=70991, encryptionId=fb94e0991b3, topicName=病毒载量)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=1c1e8876950, createdName=grace5703, createdTime=Tue Jul 21 06:50:10 CST 2020, time=2020-07-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1028311, encodeId=f82610283113e, content=新冠肺炎，疫情何时才能消失, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=公卫新人, createdTime=Sun Jul 19 18:50:10 CST 2020, time=2020-07-19, status=1, ipAttribution=)]
    2020-07-19 公卫新人

    新冠肺炎，疫情何时才能消失

    0

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