Neural Regen Res.：苓桂术甘汤治疗阿尔茨海默病的机制

《中国神经再生研究（英文版）》杂志于2012年12月36期出版的一项关于“Protective effects of Lingguizhugan decoction on amyloid-β peptide 25-35-induced cell injury: anti-inflammatory effects”的研究，观察了苓桂术甘汤对阿尔茨海默病细胞模型的保护作用及抗炎相关的机制。发现苓桂术甘汤可提高淀粉样β蛋白25-35损伤的人神经母细胞瘤细胞SH-SY5Y的活力，同时可抑制活化的小胶质细胞BV-2培养液上清中白细胞介素1β、白细胞介素6、肿瘤坏死因子α及一氧化氮的含量。作者认为，苓桂术甘汤可通过抑制小胶质细胞活化后炎性因子的过表达保护淀粉样β蛋白25-35损伤的SH-SY5Y细胞。

doi:10.3969/j.issn.1673-5374.2012.36.003
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Protective effects of Lingguizhugan decoction on amyloid-beta peptide (25–35)-induced cell injury Anti-inflammatory effects

Feifei Xi1, Feng Sang2, Chunxiang Zhou1, 3, Yun Ling1

In the present study, a human neuroblastoma cell line (SH-SY5Y) and BV-2 microglia were treated with amyloid-β peptide (25–35), as a model of Alzheimer’s disease, to evaluate the protective effects of 10-3–10-8 g/mL Lingguizhugan decoction and to examine the underlying anti-inflammatory mechanism. Lingguizhugan decoction significantly enhanced the viability of SH-SY5Y cells with amyloid-β peptide-induced injury, and lowered levels of interleukin-1β, interleukin-6, tumor necrosis factor-α and nitric oxide in the culture supernatant of activated BV-2 microglia. The effects of 10-3 g/mL Lingguizhugan decoction were more significant. These results suggest that Lingguizhugan decoction can protect SH-SY5Y cells against amyloid-β peptide (25–35)-induced injury in a dose-dependent manner by inhibiting overexpression of inflammatory factors by activated microglia.