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Open Dent J:桑树提取物具有较好的抗菌和抗炎能力

2018-05-17 MedSci MedSci原创

牙周炎是一种慢性炎症性疾病,是导致成人牙齿脱落的主要原因。关于桑树提取物(MSE)在牙周病中的抗炎活性的研究十分有限。本研究旨在探讨MSE对牙龈卟啉单胞菌脂多糖(LPS)刺激的人牙周膜成纤维细胞(hPDL)中牙周致病菌的生长和白细胞介素(IL)-6和IL-8表达的抑制作用。 通过盘扩散、最小抑制浓度和最小杀菌浓度方法测试MSE对牙龈卟啉单胞菌和伴放线聚集杆菌的抗菌活性。通过MTS测定法测定牙

牙周炎是一种慢性炎症性疾病,是导致成人牙齿脱落的主要原因。关于桑树提取物(MSE)在牙周病中的抗炎活性的研究十分有限。本研究旨在探讨MSE对牙龈卟啉单胞菌脂多糖(LPS)刺激的人牙周膜成纤维细胞(hPDL)中牙周致病菌的生长和白细胞介素(IL)-6和IL-8表达的抑制作用。

通过盘扩散、最小抑制浓度和最小杀菌浓度方法测试MSE对牙龈卟啉单胞菌和伴放线聚集杆菌的抗菌活性。通过MTS测定法测定牙龈卟啉单胞菌LPS和MSE对hPDL成纤维细胞的细胞毒性。使用逆转录-qPCR和酶联免疫吸附测定分别测量hPDL成纤维细胞中细胞因子(IL-6和IL-8)mRNA和蛋白的表达。

结果显示,MSE对牙龈卟啉单胞菌和伴放线聚集杆菌都具有抗菌活性,抑菌圈分别为10.00±0.33mm和17.33±0.58mm。MSE对牙龈卟啉单胞菌的MIC和MBC值为62.5μg/ml。MSE对放线菌伴放线菌的MIC和MBC值分别为250μg/mL和500μg/ml。牙龈卟啉单胞菌LPS显示介导hPDL成纤维细胞中促炎细胞因子的表达。然而,浓度为2.5和5.0μg/ml的MSE可显著抑制牙龈卟啉单胞菌LPS诱导的IL-6和IL-8 mRNA和蛋白质表达(p <0.05)。

本研究表明,MSE对牙龈卟啉单胞菌和伴放线聚集杆菌都具有抗菌活性。MSE抑制牙龈卟啉单胞菌LPS刺激的hPDL成纤维细胞中IL-6和IL-8的表达,表明具有抗炎作用。因此,MSE或是治疗牙周炎的潜在辅助剂。

原始出处:


Yiemwattana I, Chaisomboon N, Jamdee K. Antibacterial and Anti-inflammatory Potential of Morus alba Stem Extract. Open Dent J. 2018 Mar 30;12:265-274. doi: 10.2174/1874210601812010265. eCollection 2018.

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    2018-06-06 feather89
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    2018-05-18 kafei

    了解了谢谢

    0

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