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CLIN CHEM:急性心肌梗死患者血液中全长心肌肌钙蛋白Ⅰ及其蛋白水解片段的抗体筛选试验

2018-04-11 MedSci MedSci原创

在急性心肌梗死(AMI)患者的血液中,心肌肌钙蛋白I(cTnI)表现为具有蛋白水解片段的完整分子。cTnI的降解可能影响对其免疫检测精确程度。在这项研究中,我们确定了cTnI片段,并计算了在AMI后不同时间患者血液中的比例,以鉴别cTnI哪部分最稳定。 研究人员在植入支架前后胸痛发作后1<span style="font-family:"MS Gothic";mso-bi

在急性心肌梗死(AMI)患者的血液中,心肌肌钙蛋白I(cTnI)表现为具有蛋白水解片段的完整分子。cTnI的降解可能影响对其免疫检测精确程度。在这项研究中,我们确定了cTnI片段,并计算了在AMI后不同时间患者血液中的比例,以鉴别cTnI哪部分最稳定。 研究人员在植入支架前后胸痛发作后1〜36 h内收集AMI患者的血清标本。从血清样品中通过免疫提取cTnI及其片段,并通过用对cTnI的不同表位特异性的单克隆抗体(mAbs)和通过对cTnI的中心和末端部分特异的2免疫测定法进行Western印迹分析。 研究发现,在AMI患者血液中可以检测到完整的cTnI及其11个主要片段。连续样品中片段的比例在AMI后1-36小时期间没有显示出较大的变化。特异于氨基酸残基(aar)34和126之间的表位的mAbs可以对所有提取的cTnI染色。特异于23-36和126-196的单克隆抗体识别大约80%至90%(丰度)的cTnI。 除了特异于cTnI中心部分的mAb(氨基酸位置大约在34-126),对于相邻表位(氨基酸位置大约23-36和126-196)特异性的抗体均可用于检测,因为它们能够识别≥80%的急

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    学习学习学习

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    2018-04-13 xiaotao112411

    学习学习学习

    0

  5. 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    2018-04-13 xiaotao112411

    学习学习学习

    0

  6. 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    2018-04-13 xiaotao112411

    学习学习学习

    0

  7. 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    2018-04-13 xiaotao112411

    学习学习学习

    0

  8. 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  9. 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