Baidu
map

大牛的“家常便饭”!张锋1篇Science,“女神”1篇Nature

2016-10-12 生物探索 生物探索

在基因编辑领域,Broad研究所的张锋和加州大学伯克利分校的Jennifer Doudna都是不可否认的大牛人物。尽管因专利问题已针锋相对很久,但两人在近几年取得的多项重要研究成果是有目共睹的。在CNS上发表论文对他们来说犹如“家常便饭”。近日,这两位大牛又分别在Nature和Science杂志上发表了新成果。一起来看看,这次他们又发现了什么吧。

Nature:靶向RNA的CRISPR系统又有新发现

9月26日,在线发表于Nature杂志上的题为“Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection”的研究发现了靶向RNA的CRISPR系统中C2c2蛋白更广泛的用途。Jennifer Doudna是这一研究的通讯作者。

研究表明,RNA导向RNA切割酶(RNA-guided RNA-cutting enzyme)C2c2其实具有两种不同的RNA切割活性,而不是像先前研究中描述的只有1种。这两种不同的RNA切割活性有各自的功能:第一种是负责生产导向RNAs,使C2c2能够找到特定靶向的RNA分子;第二种是充当普通的RNA“剪刀”,用来破坏RNAs。

事实上,今年6月发表在Science杂志上一篇题为“C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector”的研究中,来自Broad研究所、MIT、NIH等机构的科学家们率先描述了这种新型靶向RNA的CRISPR系统。张锋和NIH的Eugene V. Koonin是论文的共同通讯作者。

研究中,科学家小组能够利用C2c2精准地靶向和删除特定的RNA序列,降低相应蛋白的表达水平。CRISPR/C2c2系统仅靶向RNA的这种能力提供了高通量特异性操纵RNA的新方法,也补充了CRISPR的基因编辑功能。张锋表示,C2c2的发现使CRISPR系统家族又多了一个全新的工具。


9月发表的其它4篇文章

据不完全统计,除了Nature上的这篇新论文外,Doudna 9月还相继在Molecular Cell、Nature Structural & Molecular Biology以及Nature Communications发表了3篇论文,同时还在Nature Biotechnology上发表1篇综述。具体如下表:


这篇题为“Applications of CRISPR technologies in research and beyond”的综述指出,在研究领域,基于CRISPR的基因编辑技术已经被用于多种途径,如控制转录、改变表观基因组、进行全基因组筛选和染色体成像。此外,CRISPR系统也已经被用于缓解动物的遗传疾病,可能很快会在临床上用于治疗人类眼睛和血液疾病。美国和中国已批准两项临床试验,将使用基于CRISPR/Cas9的技术治疗癌症。除了这些生物医学应用外,CRISPR技术还已被用于促进农作物生长和牲畜饲养,设计新的抗菌剂,以及控制受基因驱动的疾病携带昆虫。


Science:CRISPR技术又有新用途

说完“女神”,再来看看张锋的新研究究竟是什么。9月30日,发表在Science杂志上题为“High-resolution interrogation of functional elements in the noncoding genome”的研究中,科学家们证实,基于CRISPR的技术能够识别控制疾病相关基因的调控元件。张锋和Broad研究所的Neville E. Sanjana是这一研究的共同通讯作者。

研究中,科学家们利用一个大型sgRNA(single guide RNA )文库鉴定出了影响基因调节的非编码元件。这些基因的功能缺失突变与癌症药物 vemurafenib(用于治疗BRAF V600E突变的转移性黑色素瘤)的耐药性有关;因此,调控这些基因的启动子和增强子元件很受关注。

让科学家们惊讶的是,这一研究中鉴定出的大部分非编码调控元件利用传统技术是无法发现的。Sanjana表示:“相比蛋白质编码基因序列,我们对非编码调控元件的了解并不多。这一研究连同其它的基因编辑筛选将使我们能够发现调控这些基因组重要部分的规则。”

Sanjana的实验室目前正在开发改善CRISPR/Cas9基因组筛选能力的方法,希望能够处理更长的基因组。哈佛大学的遗传学大牛George Church表示,从100万碱基延伸至32亿碱基将是巨大的挑战之一。

值得一提的是,9月29日,发表在Science杂志上的另一篇题为“Systematic mapping of functional enhancer-promoter connections with CRISPR interference”的论文也报道了相似的研究成果。科学家们使用高通量 CRISPRi 筛选技术,在两种疾病相关基因1兆碱基(megabase)距离内发现了非编码调控元件。MD安德森癌症中心的癌症基因组研究者Traver Hart:“这是首次对基因组中增强子区域系统、直接的分析。”

原始出处:

CRISPR toolbox expanded by protein that cuts RNA in two distinct ways

Alexandra East-Seletsky,Mitchell R. O’Connell,et al Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.Nature 26 September 2016

Targeting the Noncoding Genome with CRISPR

Neville E. Sanjana, Feng Zhang,et al High-resolution interrogation of functional elements in the noncoding genome.Science  30 Sep 2016

Charles P. Fulco1, Mathias Munschauer et al.Systematic mapping of functional enhancer-promoter connections with CRISPR interference.Science 29 Sep 2016

版权声明:
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料,版权均属于梅斯医学所有。非经授权,任何媒体、网站或个人不得转载,授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章,或“梅斯号”自媒体发布的文章,仅系出于传递更多信息之目的,本站仅负责审核内容合规,其内容不代表本站立场,本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系,我们将立即进行删除处理。
在此留言
评论区 (18)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2017-02-06 1debbfffm06(暂无匿称)

    膜拜大牛

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2017-02-05 huangzh898

    牛人!

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2017-07-23 liye789132251
  4. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2016-10-17 xyfg98

    谢谢分享!

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2016-10-14 Arthur M

    大牛

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2016-10-14 jichang
  7. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2016-10-13 明月清辉

    大牛,膜拜。

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2016-10-13 孙文杰

    这么牛,学习了~

    0

  9. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2016-10-12 sophia0225

    努力学习

    0

  10. [GetPortalCommentsPageByObjectIdResponse(id=174519, encodeId=9fd51e4519f0, content=膜拜大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d32a1717978, createdName=1debbfffm06(暂无匿称), createdTime=Mon Feb 06 11:03:49 CST 2017, time=2017-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=174383, encodeId=ef5d1e438378, content=牛人!, beContent=null, objectType=article, channel=null, level=null, likeNumber=124, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20230812/e93037ddfed9425fb0316dc3e904f2f4/870c0a4383ef42ef803173fea0339be3.jpg, createdBy=27ba1551982, createdName=huangzh898, createdTime=Sun Feb 05 17:52:55 CST 2017, time=2017-02-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1881367, encodeId=8b5d188136ef1, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Sun Jul 23 19:31:00 CST 2017, time=2017-07-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148991, encodeId=5cb7148991df, content=谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fe261399236, createdName=xyfg98, createdTime=Mon Oct 17 11:25:42 CST 2016, time=2016-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=148046, encodeId=171d14804639, content=大牛, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLB2lWFzjaQjxC33RfYGdWpDovtkkdhzWmm9c4lRfGccXhIurkRaLuS5VBHDGBbAOTm2bWazV6x9wg/0, createdBy=342e1692193, createdName=Arthur M, createdTime=Fri Oct 14 08:35:13 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1375349, encodeId=9a5413e534949, content=<a href='/topic/show?id=2949159989d' target=_blank style='color:#2F92EE;'>#SCIE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15998, encryptionId=2949159989d, topicName=SCIE)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=17a3387, createdName=jichang, createdTime=Fri Oct 14 00:31:00 CST 2016, time=2016-10-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147366, encodeId=cc6d14e3662d, content=大牛,膜拜。, beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6Nx0VGA4QMDzOP8oAgnV4h51EjSfehJ9QxyiaSmw8PB56BhY8KXFSxHCibXrzcy4abrzSibXQPxJ9kzrLcJyhhB8a/0, createdBy=1d3b1672603, createdName=明月清辉, createdTime=Thu Oct 13 09:12:45 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=147323, encodeId=22f514e32309, content=这么牛,学习了~, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/y1ZJaAFo7AFqm7Hn2QNVTjOpBV9mRicUEjyBqRMnLADF1oQXJGeIVCpiaY1hJVMd1C40EhFCw4LL8sCKTx8Yuvsw/132, createdBy=af0b1960284, createdName=孙文杰, createdTime=Thu Oct 13 07:54:51 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146918, encodeId=5be4146918f9, content=努力学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3351963162, createdName=sophia0225, createdTime=Wed Oct 12 16:09:29 CST 2016, time=2016-10-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=146845, encodeId=0cdd14684539, content=基础研究还是很难很深奥, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20151226/IMG567EB873985DE3928.jpg, createdBy=ea941622301, createdName=inter158, createdTime=Wed Oct 12 14:14:00 CST 2016, time=2016-10-12, status=1, ipAttribution=)]
    2016-10-12 inter158

    基础研究还是很难很深奥

    0

相关资讯

韩春雨基因编辑技术恐遭弃用:数百个基因中重复失败

导语:因为大面积无法重复实验,作者和所在单位又不出面应对质疑声,河北科技大学副教授韩春雨领衔的NgAgo基因编辑技术的重复工作已经遇冷。自2016年5月2日在《自然-生物技术》在线发表论文,NgAgo已经问世5个月,韩春雨在论文中描述的NgAgo是一项和目前主流的“基因魔剪”CRISPR拥有同样效率的基因编辑技术,能高效地实现对特定DNA片段的敲除、加入。但在近日哥本哈根举办的“CRISPR

默克推出全新基因编辑技术Centinel

领先的科技公司默克 (Merck) 今天推出一项全新的基因编辑技术,用于对中国仓鼠卵巢细胞 (CHO) 系进行改造,从而抵御鼠细小病毒 (MVM),这在同类技术中尚属首创。

新一代基因编辑技术在南京大学问世 超越韩春雨

2016年9月15日,《Genome Biology》报道了一种基于SGN的基因编辑新技术,以结构引导的内切酶(SGN,Structure-guided nuclease)实现体内外DNA任意序列的靶向和切割。论文一作为Shu Xu,论文通信作者为南京大学医学院附属金陵医院的周国华(Guohua Zhou)研究员、南京大学模式动物研究所的赵庆顺(Qingshun Zhao)教授

韩春雨候选“中青年科技创新领军人才”,到底怎么解决韩春雨“疑云”?

继收获河北省科协副主席、“美丽河北·最美教师”头衔之后,河北科技大学副教授韩春雨正成为国家“中青年科技创新领军人才”的候选人之一。此前,7月,河北科技大学推荐韩春雨为2016年度“***奖励计划”人选候选人。
 
9月30日,河北科技大学官网一则名为《关于我校2016年国家创新人才推进计划暨“万人计划”科技创新领军人才、科技创业领军人才推荐人选的公示》的通告显示,韩春雨等四位在校教师成为“中青年科技创新领军人才”推荐人选。通告还表示,9月22日学校发布了申报通知,“校内共有4人进行申报,学校科研院对申报材料进行了严格审核”,共有4人入选推荐,公示期为3日,但没有标明如有异议,可联系何人。
 
· 2016-10-11

-->

韩春雨所在河北科大两千万设备招标被指暗箱操作

虽尚未公布中标结果,河北科技大学基因编辑技术研究中心近两千万元的进口设备采购项目已起争议。有参与投标的公司向该项目代理机构河北省国际招标有限公司提交质疑函,认为该项目在采购活动中没有执行原先公示的最低评标价法。从河北省国际招标有限公司该项目联系人李俊旭处获悉,河北省国际招标有限公司的确收到了质疑函,李俊旭表示:“有质疑很正常,我们经常遇到这个。”8月31日,中国政府采购网上公示了“河北科技大学

12位学者实名发声:未能重复韩春雨实验,呼吁第三方介入调查

10月10日晚,12位学者站了出来,决定实名公开他们“重复”韩春雨实验方法的结果:“阴性的”、“不工作”等等。这样结论的通俗表达即是,他们没能“重复”出韩春雨的实验,其实验方法“让人怀疑”。这并非第一次有人质疑韩春雨的实验方法,但愿意实名站出来,并接受公共媒体的采访,却十分罕见。今年5月初,河北科技大学副教授韩春雨在Nature Biotechnology(《自然·生物技术》)发了一篇论文声称发现

Baidu
map
Baidu
map
Baidu
map
Baidu
map