CLIN CHEM LAB MED：多中心研究评估I型前胶原蛋白n末端前肽(PINP)检测方法的一致性

2019-11-17 MedSci MedSci原创

生物化学骨转换标志物(BTM)是在细胞水平上评估骨重建的主要工具。在研究中，I型前胶原蛋白(PINP)的n端前肽被推荐作为骨形成的参考标记。

生物化学骨转换标志物(BTM)是在细胞水平上评估骨重建的主要工具。在研究中，I型前胶原蛋白(PINP)的n端前肽被推荐作为骨形成的参考标记。

本研究描述了一个多中心研究的结果，研究人员检测常规临床实验室检测血清和血浆中的PINP。从位于四个中心(雅典，希腊[GR]，哥本哈根，丹麦[DK]，列日，比利时[BE]和谢菲尔德，英国[英国])收集了到骨质疏松症诊所就诊的796名患者的血清和血浆(EDTA)样本。根据制造商的说明，用每一种可用的常规临床实验室方法对标本进行一式两份的分析。采用Passing-Bablok回归、Bland-Altman图、v型评价方法、血清和血浆标本间PINP值的一致性相关系数以及方法间的一致性相关系数来确定结果之间的一致性，同时采用广义线性模型识别可能影响两种方法之间关系的变量。

结果表明，EDTA血浆和血清均可用于PINP的检测。研究人员观察到Orion放射免疫测定法与PINP (Roche Cobas和IDS iSYS)自动化方法之间存在显著的比例偏差，但两者的结果非常相似。多变量模型并没有改善两种方法之间的良好相关性。

研究表明，通过应用校正系数或正确分配校准值，可以使PINP试验更加一致。这项工作将通过制造商和临床实验室专业人士之间的进一步合作继续改善。

原始出处：

Etienne Cavalier，Richard Eastell，A multicenter study to evaluate harmonization of assays for N-terminal propeptide of type I procollagen (PINP): a report from the IFCC-IOF Joint Committee for Bone Metabolism

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2019-12-22 zhanfl

#一致性#

76 0
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2020-01-04 luoxiaog

#多中心#

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2020-09-16 redcrab

#多中心研究#

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2019-11-19 gracezdd

#检测方法#

107 0
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2019-11-19 12498e5fm14(暂无昵称)

#胶原蛋白#

79 0
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2019-11-19 ms3994565386320060

#Med#

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CLIN CHEM LAB MED：多中心研究评估I型前胶原蛋白n末端前肽(PINP)检测方法的一致性

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