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Gut 中国佳稿| CD177+嗜中性粒细胞作为功能活化的嗜中性粒细胞对IBD负调节

2017-06-27 BMJ BMJ

性粒细胞IBD负调节
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通讯作者：刘占举  同济大学附属第十人民医院摘要中译背景：在炎性肠病（IBD）中，嗜中性粒细胞聚集在发炎粘膜上，在发病机理中发挥了重要作用。CD177在嗜中性粒细胞中表达，在发炎过程中增加。然而，CD177+嗜中性粒细胞在IBD发病机制中的作用仍然难以捉摸。材料与方法：使用定量RT-PCR，流式细胞术和免疫组化学法分析IBD患者外周血和肠粘膜中CD177的表达。分离CD177+和CD17


通讯作者：刘占举  同济大学附属第十人民医院

摘要中译

背景：在炎性肠病（IBD）中，嗜中性粒细胞聚集在发炎粘膜上，在发病机理中发挥了重要作用。CD177在嗜中性粒细胞中表达，在发炎过程中增加。然而，CD177+嗜中性粒细胞在IBD发病机制中的作用仍然难以捉摸。

材料与方法：使用定量RT-PCR，流式细胞术和免疫组化学法分析IBD患者外周血和肠粘膜中CD177的表达。分离CD177+和CD177-嗜中性粒细胞，通过RNA测序确定基因差异。用葡聚糖硫酸钠诱导CD177-/-和野生型小鼠结肠炎，以确定CD177+嗜中性粒细胞在IBD中的作用。

结果：与健康对照组相比，IBD患者外周血和炎症粘膜中的CD177+嗜中性粒细胞明显升高。RNA测序显示，来自IBD患者的CD177+和CD177-嗜中性粒细胞之间的差异基因表达与对细菌防御、过氧化氢和活性氧（ROS）的反应有关。与CD177-亚型相比，CD177+嗜中性粒细胞产生较低水平的促炎细胞因子（即干扰素-γ，白介素（IL）-6，IL-17A），但IL-22和转化生长因子-β水平较高，并且表现出增强的杀菌活性（即ROS、抗菌肽、嗜中性粒细胞的胞外捕获）。与野生型小鼠相比，CD177 - / -小鼠对DSS诱导发展了更严重的结肠炎。此外，通过CD177-嗜中性粒细胞产生的IL-22减少，CD177缺乏导致肠道屏障和抗细菌免疫受损。

结论：CD177+嗜中性粒细胞代表功能活化的人群，并通过增加杀菌活性和IL-22的产生，在IBD中发挥保护作用。靶向CD177+嗜中性粒细胞可能有益于IBD的治疗。

原始出处：
Zhou G， Yu L， Fang L， et al. CD177+neutrophils as functionally activated neutrophils negatively regulate IBD. Gut. doi：10.1136/gutjnl-2016- 313535

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  1. [GetPortalCommentsPageByObjectIdResponse(id=2028989, encodeId=60b3202898932, content=<a href='/topic/show?id=89a440214c2' target=_blank style='color:#2F92EE;'>#嗜中性粒细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=40214, encryptionId=89a440214c2, topicName=嗜中性粒细胞)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/F82CC4A89F97BD21D3DDB7AD5F2AD09D/100, createdBy=52952500022, createdName=ms6829001416292975, createdTime=Sat Aug 26 00:20:00 CST 2017, time=2017-08-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=216556, encodeId=25fa21655683, content=感谢小编为我们准备了如此丰盛的精神大餐，同时也向作者致谢！认真学习了，点赞！, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Thu Jun 29 07:15:53 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1263438, encodeId=540112634385e, content=<a href='/topic/show?id=75bc930217' target=_blank style='color:#2F92EE;'>#IBD#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=9302, encryptionId=75bc930217, topicName=IBD)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc0372, createdName=bnurmamat, createdTime=Thu Jun 29 05:20:00 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1296638, encodeId=6d7b1296638a4, content=<a href='/topic/show?id=29c82219e47' target=_blank style='color:#2F92EE;'>#中性粒细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=22197, encryptionId=29c82219e47, topicName=中性粒细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3883266, createdName=yykkxiaodou, createdTime=Thu Jun 29 05:20:00 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=216019, encodeId=5aff216019ad, content=好文章，好思路，学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=aadd1680584, createdName=蔬菜, createdTime=Tue Jun 27 16:43:11 CST 2017, time=2017-06-27, status=1, ipAttribution=)]
    2017-08-26 ms6829001416292975

    #嗜中性粒细胞#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=2028989, encodeId=60b3202898932, content=<a href='/topic/show?id=89a440214c2' target=_blank style='color:#2F92EE;'>#嗜中性粒细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=40214, encryptionId=89a440214c2, topicName=嗜中性粒细胞)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/F82CC4A89F97BD21D3DDB7AD5F2AD09D/100, createdBy=52952500022, createdName=ms6829001416292975, createdTime=Sat Aug 26 00:20:00 CST 2017, time=2017-08-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=216556, encodeId=25fa21655683, content=感谢小编为我们准备了如此丰盛的精神大餐，同时也向作者致谢！认真学习了，点赞！, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Thu Jun 29 07:15:53 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1263438, encodeId=540112634385e, content=<a href='/topic/show?id=75bc930217' target=_blank style='color:#2F92EE;'>#IBD#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=9302, encryptionId=75bc930217, topicName=IBD)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc0372, createdName=bnurmamat, createdTime=Thu Jun 29 05:20:00 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1296638, encodeId=6d7b1296638a4, content=<a href='/topic/show?id=29c82219e47' target=_blank style='color:#2F92EE;'>#中性粒细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=22197, encryptionId=29c82219e47, topicName=中性粒细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3883266, createdName=yykkxiaodou, createdTime=Thu Jun 29 05:20:00 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=216019, encodeId=5aff216019ad, content=好文章，好思路，学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=aadd1680584, createdName=蔬菜, createdTime=Tue Jun 27 16:43:11 CST 2017, time=2017-06-27, status=1, ipAttribution=)]
    2017-06-29 ylzr123

    感谢小编为我们准备了如此丰盛的精神大餐，同时也向作者致谢！认真学习了，点赞！

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=2028989, encodeId=60b3202898932, content=<a href='/topic/show?id=89a440214c2' target=_blank style='color:#2F92EE;'>#嗜中性粒细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=40214, encryptionId=89a440214c2, topicName=嗜中性粒细胞)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/F82CC4A89F97BD21D3DDB7AD5F2AD09D/100, createdBy=52952500022, createdName=ms6829001416292975, createdTime=Sat Aug 26 00:20:00 CST 2017, time=2017-08-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=216556, encodeId=25fa21655683, content=感谢小编为我们准备了如此丰盛的精神大餐，同时也向作者致谢！认真学习了，点赞！, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Thu Jun 29 07:15:53 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1263438, encodeId=540112634385e, content=<a href='/topic/show?id=75bc930217' target=_blank style='color:#2F92EE;'>#IBD#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=9302, encryptionId=75bc930217, topicName=IBD)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc0372, createdName=bnurmamat, createdTime=Thu Jun 29 05:20:00 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1296638, encodeId=6d7b1296638a4, content=<a href='/topic/show?id=29c82219e47' target=_blank style='color:#2F92EE;'>#中性粒细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=22197, encryptionId=29c82219e47, topicName=中性粒细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3883266, createdName=yykkxiaodou, createdTime=Thu Jun 29 05:20:00 CST 2017, time=2017-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=216019, encodeId=5aff216019ad, content=好文章，好思路，学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=aadd1680584, createdName=蔬菜, createdTime=Tue Jun 27 16:43:11 CST 2017, time=2017-06-27, status=1, ipAttribution=)]
    2017-06-29 bnurmamat

    #IBD#

    0

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    2017-06-29 yykkxiaodou

    #中性粒细胞#

    0

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    2017-06-27 蔬菜

    好文章，好思路，学习了

    0

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