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Heliyon：体外研究牙髓干细胞在3D支架中的表现

2018-10-09 MedSci MedSci原创

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骨移植替代物的三维多孔支架在细胞靶向和移植策略中起关键作用。这些支架提供的表面可促进干细胞的附着、存活、迁移、增殖和分化。本研究旨在评估在含有和不含羟基磷灰石的聚乳酸/聚乙醇酸支架上培养的人牙髓间充质干细胞的体外行为。 研究从前磨牙和磨牙中获得牙髓干细胞进行体外实验研究。将细胞在具有成骨分化培养基的支架上培养。在三个不同的时间点评估细胞增殖、粘附和细胞向生物材料中的成骨细胞分化（7,15和3

骨移植替代物的三维多孔支架在细胞靶向和移植策略中起关键作用。这些支架提供的表面可促进干细胞的附着、存活、迁移、增殖和分化。本研究旨在评估在含有和不含羟基磷灰石的聚乳酸/聚乙醇酸支架上培养的人牙髓间充质干细胞的体外行为。

研究从前磨牙和磨牙中获得牙髓干细胞进行体外实验研究。将细胞在具有成骨分化培养基的支架上培养。在三个不同的时间点评估细胞增殖、粘附和细胞向生物材料中的成骨细胞分化（7,15和30天）。使用Split Plot块和MANOVA模型进行数据分析。

结果显示，在7,15和30天，与PLGA支架相比，PLGA/HA支架中hDPSCs对成骨细胞谱系的分化能力更好，成骨标志物RUNX2，ALP，OPN和COL-1的表达较高。两种类型的支架之间在细胞粘附上无明显的统计学差异。

综上所述，该研究结果表明，PLGA/HA支架提供了更好的物理和化学信号，可以通过牙髓干细胞粘附、增殖和向成骨谱系分化的能力来判断。

原始出处：

Nury Tatiana Jiménez, Juan Carlos Munévar, et al., In vitro response of dental pulp stem cells in 3D scaffolds: A regenerative bone material. Heliyon. 2018 Sep; 4(9): e00775.


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    2019-03-01 jxrzshh

    #牙髓#

    0

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    2018-10-11 ms9833124466084992

    #3D#

    0

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    2018-10-11 ms6519009802973839

    #牙髓干细胞#

    0

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