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手把手教绘制回归分析结果的森林图『GraphPad Prism和Excel』

2020-06-14 龚志忠 医咖会

在本期内容中,我们来实际操练一下,介绍几款常用的软件,教大家绘制出高质量的森林图。

在本期内容中,我们来实际操练一下,介绍几款常用的软件,教大家绘制出高质量的森林图。
下面我们以2016年发表在JACC杂志上的一篇文章《A Prospective Natural History Study of Coronary Atherosclerosis Using Fractional Flow Reserve》为例,利用文章中的Table 3多因素回归分析的结果来绘制森林图。

GraphPad Prism——论文绘图必备

GraphPad Prism是一款专门为医学专业人士设计的绘图软件,同时也兼顾统计分析的功能,操作便捷,可以帮助我们轻轻松松绘制出高质量的专业图形,简直就是科研人员的福利。
下面我们以GraphPad Prism 7为例,来介绍一下如何绘制回归分析结果的森林图。
1. 新建GraphPad Prism文件
在New Table & Graph下选择Column,利用软件提供的Metanlysis(Forest)plot教程数据新建一个GraphPad Prism文件。

 

 

2. 录入数据
按照软件提供的教程数据模板,录入对应的变量名称、HR值以及95% CI。

 

3. 点击Graphs下级目录中的Metanalysis,即可发现软件已经自动生成了一个森林图雏形,但是我们还需要对图形的一些细节进行进一步调整。

 

 

4. 调整坐标轴:双击坐标轴,进入Format Axes界面
4.1 首先,在Frame and Origin选项卡中设置坐标轴的起点(即无效线的坐标点),图形的形状、大小、位置,坐标轴的颜色、厚度等参数。

 

 

4.2 然后,在X axis选项卡中设置X轴的参数,主要包括刻度范围,刻度方向、长度,X轴的起点和增量等信息,同时在Titles & Fonts选项卡中设置变量名称与Y轴的距离。

 

 

 

5. 调整图形元素:双击图片,进入Format Graph界面
5.1 首先,在Appearance选项卡中设置图形各个元素的细节,包括颜色、形状、大小、厚度等信息。

 

 

5.2 然后,在Data Sets on Graph选项卡中调整变量名称的顺序。 

 

 

此时再次点击Graphs下级目录中的Metanalysis,发现经过调整后的森林图如下图所示,已经基本符合要求了,但总觉得少一些什么。

 

6. 插入数据文件
如果想在森林图中进一步添加HR值、95% CI及对应的P值,可以在图形处单击右键,选择Insert Object → Excel Object,插入一个Excel文件,录入对应的数值即可。最终生成的森林图如下所示,就可以直接导出放在文章中了哈。

 

  

Excel——办公居家标配

Excel作为Office办公三件套的标配之一,其处理数据和绘图的功能也是非常丰富的,它的强大足够超乎你的想象。相信大家对Excel的操作已经很熟悉了,那么怎样利用Excel绘制森林图呢?
我们以Excel 2013为例,虽然Excel里并没有直接提供绘制森林图的图形模板,但我们可以另辟蹊径,通过选择“插入→ 图表 → 所有图表 → 股价图 → 收盘图”来绘制森林图的雏形,如下图所示。

 

(Excel中录入数据)

 

 

 

这是一个旋转了90°的森林图,我们在上一期内容中也介绍了类似的森林图,这种类似的图也经常能够在文献中看到,例如2018年发表在Arterioscler Thromb Vasc Biol杂志(影响因子:6.6)的一篇文章《High-Density Lipoprotein Cholesterol and Mortality Too Much of a Good Thing?》
文中为了表达HDL-C水平与全因死亡之间的关联性(HR值),就采用了这种旋转了90°的森林图来表达分析结果,是不是和上面用Excel做出来的森林图雏形很相似呢。根据这种绘图风格,小咖深深怀疑这6.6分文章里的森林图就是用Excel搞定的。。。

 

 

下面,在这个图形的基础上,我们可以对图形的一些细节进行加工,包括字体、颜色、坐标轴刻度、交叉点等等(此处省略……字),修改完之后再将图形旋转回来就OK了,如下图所示。

 

 

操作过程好像听上去很简单,不过大家可能更关心的是“此处省略……字”的这个部分,到底怎样对图形的细节进行调整呢?
没关系,为了尽可能给大家提供方便,小咖已经做好了一个Excel图表的模板(ForestPlot.crtx)安利给大家。可以把这个文件放在Excel默认的模板目录下面,小咖电脑中的目录为
(C:\Users\Administrator\AppData\Roaming\Microsoft\Templates\Charts),大家可以根据自己电脑安装的目录进行寻找。
然后,当我们再次打开Excel时,选择“插入→ 图表 → 所有图表 → 模板”,就可以看见添加进去的森林图模板了。有了这个模板,大家就可以把自己的数据直接Copy进去就大功告成啦。

 

 

此外,如果还是觉得上面的森林图显得有些单薄,我们还可以将做好的森林图再插入到Excel的表格中,把图形和数据进行结合,使得看上去更加高大上哈。

 

参考文献:

[1] J Am Coll Cardiol. 2016;68:2247-55

[2] Arterioscler Thromb Vasc Biol. 2018; 38:669-72

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    2021-07-21 张小懂

    0

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    2021-05-24 1561f846acm

    太厉害👍请问有没有软件下载链接

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1001362, encodeId=a19910013625d, content=棒, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20210721/64c48c7c5a7a4ccab29b2c0e1f334ddc/c9e1cfeabb054c538b20f66b07d5d152.jpg, createdBy=5b2a1720746, createdName=张小懂, createdTime=Wed Jul 21 11:16:32 CST 2021, time=2021-07-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1671707, encodeId=c07d16e170729, content=<a href='/topic/show?id=c56c4051902' target=_blank style='color:#2F92EE;'>#回归分析#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=40519, encryptionId=c56c4051902, topicName=回归分析)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a46f26833520, createdName=cenghis, createdTime=Fri Sep 25 20:52:44 CST 2020, time=2020-09-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=968214, encodeId=eb0d96821485, content=太厉害👍请问有没有软件下载链接, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7ff42109217, createdName=1561f846acm, createdTime=Mon May 24 19:55:01 CST 2021, time=2021-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069212, encodeId=532a206921250, content=<a href='/topic/show?id=519b1420696' target=_blank style='color:#2F92EE;'>#PHP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14206, encryptionId=519b1420696, topicName=PHP)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Mon Mar 22 21:52:44 CST 2021, time=2021-03-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=883116, encodeId=801d88311636, content=挺不错的, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=0ef75416120, createdName=ms4000001651135066, createdTime=Sun Sep 06 15:39:41 CST 2020, time=2020-09-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=881641, encodeId=bbe588164118, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=64245268311, createdName=14818eb4m67暂无昵称, createdTime=Tue Sep 01 08:08:24 CST 2020, time=2020-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1962104, encodeId=d47819621042a, content=<a href='/topic/show?id=dbe6445416' target=_blank style='color:#2F92EE;'>#CEL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4454, encryptionId=dbe6445416, topicName=CEL)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=智慧医人, createdTime=Sat Jan 02 19:52:44 CST 2021, time=2021-01-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1971029, encodeId=2ceb19e102973, content=<a href='/topic/show?id=7787e1463b' target=_blank style='color:#2F92EE;'>#Excel#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7146, encryptionId=7787e1463b, topicName=Excel)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=bcaa382, createdName=fzwish20000, createdTime=Thu Jan 07 07:52:44 CST 2021, time=2021-01-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1595462, encodeId=8e1e15954621c, content=<a href='/topic/show?id=0b7e824178' target=_blank style='color:#2F92EE;'>#Graphpad#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8241, encryptionId=0b7e824178, topicName=Graphpad)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=55683, createdName=仁医06, createdTime=Tue Jun 16 13:52:44 CST 2020, time=2020-06-16, status=1, ipAttribution=)]
    2021-03-22 jeanqiuqiu
  5. [GetPortalCommentsPageByObjectIdResponse(id=1001362, encodeId=a19910013625d, content=棒, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20210721/64c48c7c5a7a4ccab29b2c0e1f334ddc/c9e1cfeabb054c538b20f66b07d5d152.jpg, createdBy=5b2a1720746, createdName=张小懂, createdTime=Wed Jul 21 11:16:32 CST 2021, time=2021-07-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1671707, encodeId=c07d16e170729, content=<a href='/topic/show?id=c56c4051902' target=_blank style='color:#2F92EE;'>#回归分析#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=40519, encryptionId=c56c4051902, topicName=回归分析)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a46f26833520, createdName=cenghis, createdTime=Fri Sep 25 20:52:44 CST 2020, time=2020-09-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=968214, encodeId=eb0d96821485, content=太厉害👍请问有没有软件下载链接, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7ff42109217, createdName=1561f846acm, createdTime=Mon May 24 19:55:01 CST 2021, time=2021-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069212, encodeId=532a206921250, content=<a href='/topic/show?id=519b1420696' target=_blank style='color:#2F92EE;'>#PHP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14206, encryptionId=519b1420696, topicName=PHP)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Mon Mar 22 21:52:44 CST 2021, time=2021-03-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=883116, encodeId=801d88311636, content=挺不错的, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=0ef75416120, createdName=ms4000001651135066, createdTime=Sun Sep 06 15:39:41 CST 2020, time=2020-09-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=881641, encodeId=bbe588164118, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=64245268311, createdName=14818eb4m67暂无昵称, createdTime=Tue Sep 01 08:08:24 CST 2020, time=2020-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1962104, encodeId=d47819621042a, content=<a href='/topic/show?id=dbe6445416' target=_blank style='color:#2F92EE;'>#CEL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4454, encryptionId=dbe6445416, topicName=CEL)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=智慧医人, createdTime=Sat Jan 02 19:52:44 CST 2021, time=2021-01-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1971029, encodeId=2ceb19e102973, content=<a href='/topic/show?id=7787e1463b' target=_blank style='color:#2F92EE;'>#Excel#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7146, encryptionId=7787e1463b, topicName=Excel)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=bcaa382, createdName=fzwish20000, createdTime=Thu Jan 07 07:52:44 CST 2021, time=2021-01-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1595462, encodeId=8e1e15954621c, content=<a href='/topic/show?id=0b7e824178' target=_blank style='color:#2F92EE;'>#Graphpad#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8241, encryptionId=0b7e824178, topicName=Graphpad)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=55683, createdName=仁医06, createdTime=Tue Jun 16 13:52:44 CST 2020, time=2020-06-16, status=1, ipAttribution=)]
    2020-09-06 ms4000001651135066

    挺不错的

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1001362, encodeId=a19910013625d, content=棒, beContent=null, objectType=article, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20210721/64c48c7c5a7a4ccab29b2c0e1f334ddc/c9e1cfeabb054c538b20f66b07d5d152.jpg, createdBy=5b2a1720746, createdName=张小懂, createdTime=Wed Jul 21 11:16:32 CST 2021, time=2021-07-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1671707, encodeId=c07d16e170729, content=<a href='/topic/show?id=c56c4051902' target=_blank style='color:#2F92EE;'>#回归分析#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=40519, encryptionId=c56c4051902, topicName=回归分析)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a46f26833520, createdName=cenghis, createdTime=Fri Sep 25 20:52:44 CST 2020, time=2020-09-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=968214, encodeId=eb0d96821485, content=太厉害👍请问有没有软件下载链接, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7ff42109217, createdName=1561f846acm, createdTime=Mon May 24 19:55:01 CST 2021, time=2021-05-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069212, encodeId=532a206921250, content=<a href='/topic/show?id=519b1420696' target=_blank style='color:#2F92EE;'>#PHP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14206, encryptionId=519b1420696, topicName=PHP)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Mon Mar 22 21:52:44 CST 2021, time=2021-03-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=883116, encodeId=801d88311636, content=挺不错的, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=0ef75416120, createdName=ms4000001651135066, createdTime=Sun Sep 06 15:39:41 CST 2020, time=2020-09-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=881641, encodeId=bbe588164118, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=64245268311, createdName=14818eb4m67暂无昵称, createdTime=Tue Sep 01 08:08:24 CST 2020, time=2020-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1962104, encodeId=d47819621042a, content=<a href='/topic/show?id=dbe6445416' target=_blank style='color:#2F92EE;'>#CEL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4454, encryptionId=dbe6445416, topicName=CEL)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=智慧医人, createdTime=Sat Jan 02 19:52:44 CST 2021, time=2021-01-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1971029, encodeId=2ceb19e102973, content=<a href='/topic/show?id=7787e1463b' target=_blank style='color:#2F92EE;'>#Excel#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7146, encryptionId=7787e1463b, topicName=Excel)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=bcaa382, createdName=fzwish20000, createdTime=Thu Jan 07 07:52:44 CST 2021, time=2021-01-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1595462, encodeId=8e1e15954621c, content=<a href='/topic/show?id=0b7e824178' target=_blank style='color:#2F92EE;'>#Graphpad#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8241, encryptionId=0b7e824178, topicName=Graphpad)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=55683, createdName=仁医06, createdTime=Tue Jun 16 13:52:44 CST 2020, time=2020-06-16, status=1, ipAttribution=)]
    2020-09-01 14818eb4m67暂无昵称

    学习

    0

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    2021-01-02 智慧医人
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    2021-01-07 fzwish20000
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    2020-06-16 仁医06

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