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Biofouling:香芹酚和松油烯-4-醇可有效对抗植体表面的菌膜

2018-09-22 MedSci MedSci原创

本研究评估了香芹酚和松油烯-4-醇对牙龈卟啉单胞菌和具核梭杆菌的抗菌性能及其对成纤维细胞的细胞毒作用。 首先评估香芹酚和松油烯-4-醇的最小抑制浓度(MIC)和最小杀菌浓度(MBC)。通过XTT测定评估最小生物膜抑制浓度(MBIC)。定量钛表面上的生物膜(CFU ml-1),通过共聚焦激光扫描显微镜(CLSM)和MTT的细胞毒活性评估。 结果显示,对于牙龈卟啉单胞菌和具核梭杆菌,香芹酚

本研究评估了香芹酚和松油烯-4-醇对牙龈卟啉单胞菌和具核梭杆菌的抗菌性能及其对成纤维细胞的细胞毒作用。

首先评估香芹酚和松油烯-4-醇的最小抑制浓度(MIC)和最小杀菌浓度(MBC)。通过XTT测定评估最小生物膜抑制浓度(MBIC)。定量钛表面上的生物膜(CFU ml-1),通过共聚焦激光扫描显微镜(CLSM)和MTT的细胞毒活性评估。

结果显示,对于牙龈卟啉单胞菌和具核梭杆菌,香芹酚的MIC和MBC为0.007%和0.002%,松油烯-4-醇均为0.06%。对于牙龈卟啉单胞菌和具核梭杆菌,香芹酚的MBIC为0.03%和0.06%,松油烯-4-醇的MBIC为0.06%和0.24%。

使用香芹酚(0.26%,0.06%)和松油烯-4-醇(0.95%,0.24%)具有抗生物膜活性,并显示出与氯己定(CHX)相似的细胞毒活性。然而,松油烯-4-醇(0.24%)处理组的细胞活力更高。香芹酚和松油烯-4-醇在减少生物膜方面显示出抗菌活性。此外,研究人员还观察到CHX样细胞毒性。

原始出处:

Maquera-Huacho PM, Tonon CC, et al., In vitro antibacterial and cytotoxic activities of carvacrol and terpinen-4-ol against biofilm formation on titanium implant surfaces. Biofouling. 2018 Sep 6:1-11. doi: 10.1080/08927014.2018.1485892.

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