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Density separation of petrous bone powders for optimized ancient DNA yields

期刊: GENOME RESEARCH, 2023; 33 (4)

Density separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply ......

Accurate sequencing of DNA motifs able to form alternative (non-B) structures

期刊: GENOME RESEARCH, 2023; 33 (6)

Approximately 13% of the human genome at certain motifs have the potential to form noncanonical (non-B) DNA structures (e.g., G-quadruplexes, crucifor......

A temporal in vivo catalog of chromatin accessibility and expression profiles in pineoblastoma reveals a prevalent role for repressor elements

期刊: GENOME RESEARCH, 2023; 33 (2)

Pediatric pineoblastomas (PBs) are rare and aggressive tumors of grade IV histology. Although some oncogenic drivers are characterized, including germ......

Robust analysis of prokaryotic pangenome gene gain and loss rates with Panstripe

期刊: GENOME RESEARCH, 2023; 33 (1)

Horizontal gene transfer (HGT) plays a critical role in the evolution and diversification of many microbial species. The resulting dynamics of gene ga......

Enhancers display constrained sequence flexibility and context-specific modulation of motif function

期刊: GENOME RESEARCH, 2023; 33 (3)

The information about when and where each gene is to be expressed is mainly encoded in the DNA sequence of enhancers, sequence elements that comprise ......

Aligning distant sequences to graphs using long seed sketches

期刊: GENOME RESEARCH, 2023; 33 (7)

Sequence-to-graph alignment is crucial for applications such as variant genotyping, read error correction, and genome assembly. We propose a novel see......

Extremely fast construction and querying of compacted and colored de Bruijn graphs with GGCAT

期刊: GENOME RESEARCH, 2023; 33 (7)

Compacted de Bruijn graphs are one of the most fundamental data structures in computational genomics. Colored compacted de Bruijn graphs are a variant......

De novo transcriptome assembly of mouse male germ cells reveals novel genes, stage-specific bidirectional promoter activity, and noncoding RNA expression

期刊: GENOME RESEARCH, 2023; 33 (12)

In mammals, the adult testis is the tissue with the highest diversity in gene expression. Much of that diversity is attributed to germ cells, primaril......

Spatial transcriptomics reveals asymmetric cellular responses to injury in the regenerating spiny mouse (Acomys) ear

期刊: GENOME RESEARCH, 2023; 33 (8)

In contrast to other mammals, the spiny mouse (Acomys) regenerates skin and ear tissue, which includes hair follicles, glands, and cartilage, in a sca......

Historical RNA expression profiles from the extinct Tasmanian tiger

期刊: GENOME RESEARCH, 2023; 33 (8)

Paleogenomics continues to yield valuable insights into the evolution, population dynamics, and ecology of our ancestors and other extinct species. Ho......

The fine-scale recombination rate variation and associations with genomic features in a butterfly

期刊: GENOME RESEARCH, 2023; 33 (5)

Recombination is a key molecular mechanism that has profound implications on both micro- and macroevolutionary processes. However, the determinants of......

Dynamic modulation of genomic enhancer elements in the suprachiasmatic nucleus, the site of the mammalian circadian clock

期刊: GENOME RESEARCH, 2023; 33 (5)

The mammalian suprachiasmatic nucleus (SCN), located in the ventral hypothalamus, synchronizes and maintains daily cellular and physiological rhythms ......

An organism-wide ATAC-seq peak catalog for the bovine and its use to identify regulatory variants

期刊: GENOME RESEARCH, 2023; 33 (10)

We report the generation of an organism-wide catalog of 976,813 cis-acting regulatory elements for the bovine detected by the assay for transposase ac......

Variation in mutation, recombination, and transposition rates in Drosophila melanogaster and Drosophila simulans

期刊: GENOME RESEARCH, 2023; 33 (4)

The rates of mutation, recombination, and transposition are core parameters in models of evolution. They impact genetic diversity, responses to ongoin......

The human genome contains over a million autonomous exons

期刊: GENOME RESEARCH, 2023; 33 (11)

Mammalian mRNA and lncRNA exons are often small compared to introns. The exon definition model predicts that exons splice autonomously, dependent on p......

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