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Initiating Method Development with Scouting Gradients- Where to Begin and How to Proceed?

期刊: LCGC EUROPE, 2023; 36 (7)

With so many options for method parameters to adjust during method development, identifying a starting point can be intimidating. Starting with scouti......

Using Thermodynamics and Simulations to Understand Selectivity and Retention in Gas Chromatography

期刊: LCGC EUROPE, 2023; 36 (2)

Retention and selectivity in gas chromatography (GC) occur because of the thermodynamics of the phase transition from the vapour phase following sampl......

A Rapid, Automated Extraction Platform to Assess Drug Product Potency by Online Liquid Chromatography

期刊: LCGC EUROPE, 2023; 36 (3)

We present a new automated active pharmaceutical ingredient (API) extraction approach with integrated analysis by online liquid chromatography (LC) to......

The Role of Separation Techniques in the Analysis of mRNA Therapeutic Drug Substances and Drug Products

期刊: LCGC EUROPE, 2023; 36 (2)

Messenger ribonucleic acids (mRNA) therapeutics are becoming more widespread pharmaceutical tools to treat a wide range of diseases or infections, as ......

What Are Options to Improve My Separation? Part 3: Options to Consider to Improve Resolution of Complex Mixtures

期刊: LCGC EUROPE, 2023; 36 (6)

Many high performance liquid chromatography (HPLC) users are confronted with questions about how to improve upon the performance of an existing or rec......

2D-LC-MS Approaches for the Analysis of In-Process Samples and for the Characterization of mAbs in a Regulated Environment

期刊: LCGC EUROPE, 2023; 36 (9)

Biologics, and in particular monoclonal antibodies (mAbs), are an important class of therapeutics, and their market share keeps growing. The productio......

The Evolution of LC Troubleshooting: Protective Devices and Column Repair

期刊: LCGC EUROPE, 2023; 36 (9)

As chromatography technologies change, our approaches to using them in ways that value reliability, as well as the ways we should approach troubleshoo......

Does High Polarity Mean High Retention on Stationary Phases in Gas Chromatography?

期刊: LCGC EUROPE, 2023; 36 (1)

Stationary phase chemistry, polarity, and selectivity have been of ongoing interest since the inception of gas chromatography (GC) in the 1950s. In th......

Online Solid-Phase Extraction-Gas Chromatography-Flame Ionization Detection System for Monitoring Contaminants at Parts-Per-Trillion Concentrations in Process Waters

期刊: LCGC EUROPE, 2023; 36 (1)

Online monitoring of odour and taste components that occur at parts-per-trillion (ppt) levels in industrial process waters requires specialized analyt......

How Chemistry Determines Separations: Influence of Selectivity on Resolution

期刊: LCGC EUROPE, 2023; 36 (3)

Resolution in chromatography is influenced by retention, engineering, and selectivity. Retention relates to how long analytes are in the column; gener......

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