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Bioinertization of NanoLC/MS/MS Systems by Depleting Metal Ions From the Mobile Phases for Phosphoproteomics

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (5)

We have successfully developed a bioinertized nanoflow LC/MS/MS (nanoLC/MS/MS) system for the highly sensi-tive analysis of phosphopeptides by depleti......

Impact of Asialoglycoprotein Receptor and Mannose Receptor Deficiency on Murine Plasma N-glycome Profiles

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (9)

The asialoglycoprotein receptor (ASGPR) and the mannose receptor C -type 1 (MRC1) are well known for their selective recognition and clearance of circ......

Integrated Immunopeptidomics and Proteomics Study of SARS-CoV-2-Infected Calu-3 Cells Reveals Dynamic Changes in Allele-specific HLA Abundance and Antigen Presentation

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (10)

We present an integrated immunopeptidomics and proteomics study of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection to comprehen......

Glycoproteomics of a Single Protein: Revealing Tens of Thousands of Myozyme Glycoforms by Hybrid HPLC-MS Approaches

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (9)

Characterization of highly glycosylated biopharmaceuticals by mass spectrometry is challenging because of the huge chemical space of coexistent glycof......

Uterine Fluid Extracellular Vesicles Proteome Is Altered During the Estrous Cycle

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (11)

Uterine environment is tightly and finely regulated via various signaling pathways mediated through endocrine, exocrine, autocrine, juxtacrine, and pa......

Increasing Complexity of the N-Glycome During Caenorhabditis Development

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (3)

Caenorhabditis elegans is a frequently employed genetic model organism and has been the object of a wide range of developmental, genetic, proteomic, a......

Accurate Label-Free Quantification by directLFQ to Compare Unlimited Numbers of Proteomes

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (7)

Recent advances in mass spectrometry-based proteomics enable the acquisition of increasingly large datasets within relatively short times, which expos......

Uncovering Protein Networks in Cardiovascular Proteomics

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (8)

Biological networks have been widely used in many different diseases to identify potential biomarkers and design drug targets. In the present review, ......

Synchro-PASEF Allows Precursor-Specific Fragment Ion Extraction and Interference Removal in Data-Independent Acquisition

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (2)

Data-independent acquisition (DIA) methods have become increasingly popular in mass spectrometry-based prote-omics because they enable continuous acqu......

Proteomic Characterization of Acute Myeloid Leukemia for Precision Medicine

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (4)

Acute myeloid leukemia (AML) is a highly heterogeneous cancer of the hematopoietic system with no cure for most patients. In addition to chemotherapy,......

An Introduction to Analytical Challenges, Approaches, and Applications in Mass Spectrometry-Based Secretomics

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (9)

The active release of proteins into the extracellular space and the proteolytic cleavage of cell surface proteins are key processes that coordinate an......

PaxDb 5.0: Curated Protein Quantification Data Suggests Adaptive Proteome Changes in Yeasts

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (10)

The Protein Abundances Across Organisms database (PaxDb) is an integrative metaresource dedicated to protein abundance levels, in tissue -specific or ......

Application of Liquid-Liquid Extraction for N-terminal Myristoylation Proteomics

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (12)

Proteins can be modified by lipids in various ways, for example, by myristoylation, palmitoylation, farnesylation, and geranylgeranylation-these proce......

Biochemical Deconstruction and Reconstruction of Nuclear Matrix Reveals the Layers of Nuclear Organization

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (12)

Nuclear matrix (NuMat) is the fraction of the eukaryotic nucleus insoluble to detergents and high -salt extractions that manifests as a pan -nuclear f......

Large-Scale Immunopeptidome Analysis Reveals Recurrent Posttranslational Splicing of Cancer- and Immune-Associated Genes

期刊: MOLECULAR & CELLULAR PROTEOMICS, 2023; 22 (4)

Posttranslational spliced peptides (PTSPs) are a unique class of peptides that have been found to be presented by HLA class-I molecules in cancer. Thu......

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