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Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 183 ()

Efficient preparation of libraries is the key step of next-generation sequencing (NGS) methods. Tn5 transposase enables simple, robust and highly effi......

A simple protocol to purify human TFIID free of the MED26 subunit of mediator complex

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 184 ()

The general transcription factor TFIID is a multiprotein complex that is essential for specific transcription initiation by RNA polymerase II. It is c......

A scalable and reproducible preparation for the antitumor protein TLC, a human-derived telomerase inhibitor

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 187 ()

Telomerase, which is overexpressed in approximately 90% of liver cancer cells, is an ideal target for anti-liver cancer therapy. LPTS, a putative live......

Expression and characterization of a thermotolerant and pH-stable hyaluronate lyase from Thermasporomyces composti DSM22891

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 182 ()

Hyaluronate lyases have received extensive attention due to their applications in medical science, drug and biochemical engineering. However, few ther......

Preparation of a polyclonal antibody against the non-structural protein, NSs of SFTSV

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 184 ()

Severe fever with thrombocytopenia syndrome virus (SFTSV) is newly discovered virus which is the member of the order Bunyavirales, family phenuivirida......

Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 186 ()

For recombinant proteins produced in Chinese hamster ovary (CHO) cells, fragmentation is a common phenomenon that results in generation of product-rel......

Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 187 ()

Zearalenone (ZEN), one of the most dangerous mycotoxins, causes enormous economic losses in the food and feed industries. To solve the problem of ZEN ......

The structure and properties of MFG-E8 and the In vitro assessment of its toxic effects on myoblast cells

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 178 ()

Four high-molecular-weight protein fractions of milk fat globule membrane (MFGM) were isolated from bovine milk. Sodium dodecyl sulfate-polyacrylamide......

Evaluating a new mixed-mode resin Diamond MMC Mustang using Capto MMC ImpRes as a benchmark

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 186 ()

Diamond MMC Mustang is a relatively new mixed-mode resin, which mediates both cation exchange and hydrophobic interactions. In this work, we evaluated......

Identification of chicken-derived scFv against N-glycolylneuraminic acid retrieved from an immune library by phage display

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 182 ()

N-glycolylneuraminic acid (Neu5Gc) is a type of sialic acid, it can be synthesized by a range of mammals except chickens and healthy human. After ente......

Cloning and characterization of a novel DNase gene from Trichogramma pretiosum

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 185 ()

DNase is a powerful tool for a series of molecular biology applications. Developing a strategy for large-scale production of DNase with high purity an......

Expression and purification of a recombinant ELRL-MAP30 with dual-targeting anti-tumor bioactivity

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 185 ()

MAP30 (Momordica antiviral protein 30kD) is a single-chain I-type ribosome inactivating protein with a variety of biological activities, including ant......

A comparative study and evaluation of anti-EGFR nanobodies expressed in Pichia pastoris and Escherichia coli as antitumor moieties

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 184 ()

Anti-EGFR nanobodies have been successfully applied as antitumor moieties in the photodynamic therapy and drug delivery systems. But the yields of nan......

Enzymatic properties of an efficient glucan branching enzyme and its potential application in starch modification

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 178 ()

Glucan branching enzymes (GBEs, EC 2.4.1.18) catalyze the formation of a-1,6-linked branch in starch, which is important for the starch modification w......

Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2021; 183 ()

The poor grain filling of inferior spikelets (IS) situated on the lower secondary rachis branch leads to a remarkable decrease in rice yield and quali......

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