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Predicting essential genes of 37 prokaryotes by combining information-theoretic features

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 188 ()

Essential genes are required for the reproduction and survival of an organism. Rapid identification of essential genes has practical application value......

Establishment and performance evaluation of multiplex PCR-dipstick DNA chromatography assay for simultaneous diagnosis of four sexually transmitted pathogens

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 186 ()

Introduction: Rapid, sensitive, and specific diagnostic methods are indispensable for sexually transmitted infections (STIs). In this study, a multipl......

Design of potentially universal SSU primers in myxomycetes using next-generation sequencing

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 184 ()

Unlike fungi, which have a universally accepted barcode marker, universal primers still lack in myxomycetes. Typically, DNA barcode primers were desig......

Effect of different sample treatment methods on Low Endotoxin Recovery Phenomenon

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 186 ()

Endotoxin is a kind of lipopolysaccharide that exits on the cell wall of Gram-negative bacteria. It can cause fever, shock or even death when is deliv......

A novel bioluminescent approach to the loop-mediated isothermal amplification-based detection of Lactobacillus salivarius in feed samples

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 187 ()

Coupling loop-mediated isothermal amplification (LAMP) with a bioluminescent assay in real-time (LAMPBART) is a strategy that can be readily leveraged......

Rapid detection of fungi from blood samples of patients with candidemia using modified calcofluor white stain

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 184 ()

We reported a modified CFW assay for rapid detection of fungi in blood samples and evaluated its efficacy in vivo and in vitro. The positive rate, sen......

Comparison of the efficacy of metagenomic next-generation sequencing and Xpert MTB/RIF in the diagnosis of tuberculous meningitis

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 180 ()

Metagenomic next-generation sequencing (mNGS) is an emerging sequence-based method that detects genomic information of pathogenic microorganisms from ......

MALDI-TOF MS platform combined with machine learning to establish a model for rapid identification of methicillin-resistant Staphylococcus aureus

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 180 ()

MALDI-TOF MS is an effective potential tool to distinguish between MSSA and MRSA. By combining the ClinProTools3.0 software and manual grouping interv......

Detection of Exiguobacterium spp. and E. acetylicum on fresh-cut leafy vegetables by a multiplex PCR assay

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 180 ()

Aims: To identify the main spoilage bacterium on fresh-cut leafy vegetables and establish a multiplex PCR assay. Methods and results: Based on physiol......

A low pH-based rapid and direct colorimetric sensing of bacteria using unmodified gold nanoparticles

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 180 ()

Herein we report a novel strategy for the detection of bacteria using unfunctionalized gold nanoparticles (AuNPs), which was utilized as a colorimetri......

A sensitive EZMTT method provides microscale, quantitative and high-throughput evaluation of drug efficacy in the treatment of Mycobacterium tuberculosis infectious diseases

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 181 ()

Drug resistance has become a serious public health problem in mycobacterial infectious diseases. Here, we investigated a water soluble tetrazolium sal......

Sensitive and rapid detection of cytolethal distending toxin encoding genes in Providencia alcalifaciens

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 181 ()

Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplificati......

Template length, concentration and guanidine and cytosine content influence on multiple displacement amplification efficiency

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 181 ()

Detection of low abundance human health pathogens in environmental samples is a challenge for water monitoring. This limitation can be overcome by the......

Agrobacterium tumefaciens-mediated transformation of Coniella granati

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 182 ()

Pomegranate fruit rot caused by Coniella granati is among the most devastating diseases threatening pomegranate production. The pathogenic mechanism o......

Accurate and sensitive analysis of Staphylococcus aureus through CRISPR-Cas12a based recycling signal amplification cascades for early diagnosis of skin and soft tissue infections

期刊: JOURNAL OF MICROBIOLOGICAL METHODS, 2021; 183 ()

Early-diagnosis and treatments of skin and soft tissue infections remain a huge challenge due to the difficulties in the detection of trace amounts of......

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