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A cocktail of protein engineering strategies: Breaking the enzyme bottleneck one by one for high UTP production in vitro

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (6)

The pyrimidine metabolic pathway is tightly regulated in microorganisms, allowing limited success in metabolic engineering for the production of pathw......

JIF:4.404

Physiological metabolic topology analysis of Halomonas elongata DSM 2581(T) in response to sodium chloride stress

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (12)

The halophilic bacterium Halomonas elongata DSM 2581(T) generally adapts well to high level of salinity by biosynthesizing ectoine, which functions as......

JIF:4.404

Reassessing acetyl-CoA supply and NADPH availability for mevalonate biosynthesis from glycerol in Escherichia coli

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (10)

Mevalonate is an important platform compound for the biosynthesis of isoprenoids. It can be synthesized from acetyl-CoA in the presence of nicotinamid......

JIF:4.404

In silico and in vivo studies of the effect of surface curvature on the osteoconduction of porous scaffolds

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (2)

Recent evidence shows that the curvature of porous scaffold plays a significant role in guiding tissue regeneration. However, the underlying mechanism......

JIF:4.404

Comparative genomic and transcriptomic analysis guides to further enhance the biosynthesis of erythromycin by an overproducer

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (6)

Omics approaches have been applied to understand the boosted productivity of natural products by industrial high-producing microorganisms. Here, with ......

JIF:4.404

Biosynthesis of allantoin in Escherichia coli via screening a highly effective urate oxidase

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (9)

Allantoin is an important fine chemical that can be widely used in pharmaceutical, cosmetic and agricultural industries. Currently, allantoin is mainl......

JIF:4.404

Regulating substrate preference of phosphatase by reshaping the cap domain for multi-enzymatic biosynthesis of high-purity monosaccharides

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (12)

Phosphatases are a class of enzymes catalyzing the cleavage of monophosphate ester bonds from the phosphorylated substrates. They have important appli......

JIF:4.404

Production of retinoic acid by engineered Saccharomyces cerevisiae using an endogenous aldehyde dehydrogenase

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (11)

Retinoic acid (RA), a vitamin A (retinol)-derived lipophilic compound, is involved in various physiological functions. The demand for RA is growing in......

JIF:4.404

Engineering Escherichia coli asymmetry distribution-based synthetic consortium for shikimate production

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (11)

Microbial consortia constitute a promising tool for achieving high-value chemical bio-production. However, customizing the consortium ratio remains ch......

JIF:4.404

Developing Rhodococcus opacus and Sphingobium sp. coculture systems for valorization of lignin-derived dimers

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (11)

Bioconversion is being regarded as a promising way for lignin valorization because it enables funneling diverse lignin components into single compound......

JIF:4.404

Dynamic alterations of metabolites revealed the vascularization progression of bioengineered liver

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (10)

Vascularization is a critical but challenging process in developing functional bioengineered livers with the decellularized liver scaffolds (DLSs) and......

JIF:4.404

Engineering of reaction specificity, enantioselectivity, and catalytic activity of nitrilase for highly efficient synthesis of pregabalin precursor

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (9)

Simultaneous evolution of multiple enzyme properties remains challenging in protein engineering. A chimeric nitrilase (BaNITM0) with high activity tow......

JIF:4.404

Enhancing thermal stability and lytic activity of phage lysin PlyAB1 from Acinetobacter baumannii

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (10)

With the increasingly serious drug resistance of Acinetobacter baumannii, there is an increasingly urgent need for new antibacterial drugs. Phage lysi......

JIF:4.404

3D printing of self-standing and vascular supportive multimaterial hydrogel structures for organ engineering

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (1)

Three dimensional printable formulation of self-standing and vascular-supportive structures using multi-materials suitable for organ engineering is of......

JIF:4.404

A label-free and signal-amplifiable assay method for colorimetric detection of carcinoembryonic antigen

期刊: BIOTECHNOLOGY AND BIOENGINEERING, 2022; 119 (2)

In this work, an innovative colorimetric assay method for the determination of carcinoembryonic antigen is developed with aptamer probes utilized as r......

JIF:4.404

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