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Physical activity, confidence and quality of life among cancer patient-carer dyads

期刊: SPORTS MEDICINE-OPEN, 2021; 7 (1)

Background Physical activity (PA) has been positively associated with health-related quality of life (HRQoL) among cancer patients and family caregive......

Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes

期刊: BIO-PROTOCOL, 2020; 10 (1)

Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs ......

Bone-in-culture Array to Model Bone Metastasis in ex vivo Condition

期刊: BIO-PROTOCOL, 2020; 10 (2)

Bone is the most frequently affected organ by metastases of breast cancer and prostate cancer. Our knowledge on bone metastasis is extremely limited d......

Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH

期刊: BIO-PROTOCOL, 2020; 10 (3)

Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization......

Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands

期刊: BIO-PROTOCOL, 2020; 10 (4)

Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the succ......

Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana

期刊: BIO-PROTOCOL, 2020; 10 (5)

Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultura......

Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network

期刊: BIO-PROTOCOL, 2020; 10 (5)

Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim......

Contemporaneous Measurement of Outer and Inner Membrane Permeability in Gram-negative Bacteria

期刊: BIO-PROTOCOL, 2020; 10 (5)

The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilizati......

Purification of Rice Stripe Virus

期刊: BIO-PROTOCOL, 2020; 10 (6)

Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purificat......

Murine Acute Pneumonia Model of Pseudomonas aeruginosa Lung Infection

期刊: BIO-PROTOCOL, 2020; 10 (21)

Animal infection models play significant roles in studying bacterial pathogenic mechanisms, host pathogen interaction as well as evaluating drug and v......

K+ Release Assay and K+ Measurement in Oocyte Assay

期刊: BIO-PROTOCOL, 2020; 10 (21)

Xenopus oocyte is a powerful system for the exogenous expression and functional characterization of plant membrane transport proteins. Until now, a nu......

Long-distance Transport in Bacterial Swarms Revealed by Single Nanoparticle Tracking

期刊: BIO-PROTOCOL, 2020; 10 (21)

During swarming, high density flagella-driven bacteria migrate collectively in a swirling pattern on wet agar surfaces, immersed in a thin viscous flu......

Precise Targeting of Single Microelectrodes to Orientation Pinwheel Centers

期刊: BIO-PROTOCOL, 2020; 10 (11)

In the mammalian visual system, early stages of visual form perception begin with orientation selective neurons in primary visual cortex (V1). In many......

Extracellular Vesicles Tracking and Quantification Using CT and Optical Imaging in Rats

期刊: BIO-PROTOCOL, 2020; 10 (11)

Exosomes, a subtype of extracellular vesicles, are nanovesicles of endocytic origin. Exosomes contain a plethora of proteins, lipids, and genetic mate......

Pea Aphid Rearing, Bacterial Infection and Hemocyte Phagocytosis Assay

期刊: BIO-PROTOCOL, 2020; 10 (24)

Insects rely on the simple but effective innate immune system to combat infection. Cellular and humoral responses are interconnected and synergistic i......

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