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Soluble Expression, One-Step Purification and Characterization of Recombinant Human Growth Hormone Fused with ompA3 in Escherichia coli

Zhou, ZR; Huang, W; Liu, KJ; Lin, FL; Wang, XL; Wang, F; Jiang, RW

Jiang, RW (corresponding author), Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constituent, Int Cooperat Lab Tradit Chinese Med Modernizat &, Chines Minist Educ, Guangzhou 510632, Peoples R China.

PROTEIN AND PEPTIDE LETTERS, 2021; 28 (5): 533

Abstract

Results: High-level expression (800 mu g/mL) was achieved by induction with 0.5 mM IPTG at 30 degrees C for 10 hours. The purity of hGH was over 90%. ......

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