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Mol Cancer：研究揭示LncRNA RP11-436H11.5促进肾细胞癌增殖和侵袭的机制

2017-11-03 MedSci MedSci原创

肾细胞癌lncRNA
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来越多的证据表明，长链非编码RNAs（lncRNAs）在肿瘤发生中起着至关重要的作用。本研究中，研究人员报告了一种新型lncRNA，RP11-436H11.5，通过海绵miR-335-5p调节肾细胞癌（RCC）的细胞增殖和侵袭。通过qRT-PCR测定RCC组织中的lncRNA RP11-436H11.5的表达。通过细胞增殖测定和transwell侵袭测定来测量RCC细胞的增殖和侵袭。通过蛋白质印迹

来越多的证据表明，长链非编码RNAs（lncRNAs）在肿瘤发生中起着至关重要的作用。本研究中，研究人员报告了一种新型lncRNA，RP11-436H11.5，通过海绵miR-335-5p调节肾细胞癌（RCC）的细胞增殖和侵袭。

通过qRT-PCR测定RCC组织中的lncRNA RP11-436H11.5的表达。通过细胞增殖测定和transwell侵袭测定来测量RCC细胞的增殖和侵袭。通过蛋白质印迹分析检测BCL-W的表达。通过荧光素酶报告基因测定和RNA下拉测定来测量lncRNA RP11-436H11.5和miR-335-5p之间的相互作用。体内实验用于检测肿瘤形成。

qRT-PCR结果显示lncRNA RP11-436H11.5在RCC组织中的表达高于癌旁正常肾组织。生存分析结果显示，与lncRNA RP11-436H11.5低表达组患者相比， lncRNA RP11-436H11.5高表达的患者结局明显恶化。lncRNA RP11-436H11.5的下调抑制了RCC细胞在体外和体内的增殖和侵袭。萤光素酶报告基因检测结果表明，lncRNA RP11-436H11.5通过调节miR-335-5p的表达增强BCL-W的表达。LncRNA RP11-436H11.5可以作为miR-335-5p诱饵来抑制BCL-W的表达。

总之，该研究结果表明，LncRNA RP11-436H11.5可作为竞争性内源性RNA促进RCC细胞的增殖和侵袭，或可作为抑制RCC进展的治疗药物。

原始出处：

Kefeng Wang, Wei Jin, Yan Song, Xiang Fei. LncRNA RP11-436H11.5, functioning as a competitive endogenous RNA, upregulates BCL-W expression by sponging miR-335-5p and promotes proliferation and invasion in renal cell carcinoma. Mol Cancer. 2017; 16: 166. Published online 2017 Oct 25. doi: 10.1186/s12943-017-0735-3

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  1. [GetPortalCommentsPageByObjectIdResponse(id=1978598, encodeId=483d19e859808, content=<a href='/topic/show?id=3b90e79787f' target=_blank style='color:#2F92EE;'>#细胞癌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=77, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77978, encryptionId=3b90e79787f, topicName=细胞癌)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e457184, createdName=qingting, createdTime=Wed Feb 14 18:00:00 CST 2018, time=2018-02-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2005662, encodeId=f26d20056620e, content=<a href='/topic/show?id=6ff1525e13' target=_blank style='color:#2F92EE;'>#CRN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5257, encryptionId=6ff1525e13, topicName=CRN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=35fc2500023, createdName=yanjie6253_56072842, createdTime=Wed Feb 28 11:00:00 CST 2018, time=2018-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260149, encodeId=508426014930, content=谢谢了.学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=30331616752, createdName=txqjm, createdTime=Fri Nov 10 01:45:18 CST 2017, time=2017-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1301880, encodeId=43471301880c6, content=<a href='/topic/show?id=b469109e41d' target=_blank style='color:#2F92EE;'>#lncRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10974, encryptionId=b469109e41d, topicName=lncRNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2317301, createdName=xzw113, createdTime=Sun Nov 05 13:00:00 CST 2017, time=2017-11-05, status=1, ipAttribution=)]
    2018-02-14 qingting

    #细胞癌#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1978598, encodeId=483d19e859808, content=<a href='/topic/show?id=3b90e79787f' target=_blank style='color:#2F92EE;'>#细胞癌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=77, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77978, encryptionId=3b90e79787f, topicName=细胞癌)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e457184, createdName=qingting, createdTime=Wed Feb 14 18:00:00 CST 2018, time=2018-02-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2005662, encodeId=f26d20056620e, content=<a href='/topic/show?id=6ff1525e13' target=_blank style='color:#2F92EE;'>#CRN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5257, encryptionId=6ff1525e13, topicName=CRN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=35fc2500023, createdName=yanjie6253_56072842, createdTime=Wed Feb 28 11:00:00 CST 2018, time=2018-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260149, encodeId=508426014930, content=谢谢了.学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=30331616752, createdName=txqjm, createdTime=Fri Nov 10 01:45:18 CST 2017, time=2017-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1301880, encodeId=43471301880c6, content=<a href='/topic/show?id=b469109e41d' target=_blank style='color:#2F92EE;'>#lncRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10974, encryptionId=b469109e41d, topicName=lncRNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2317301, createdName=xzw113, createdTime=Sun Nov 05 13:00:00 CST 2017, time=2017-11-05, status=1, ipAttribution=)]
    2018-02-28 yanjie6253_56072842

    #CRN#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1978598, encodeId=483d19e859808, content=<a href='/topic/show?id=3b90e79787f' target=_blank style='color:#2F92EE;'>#细胞癌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=77, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77978, encryptionId=3b90e79787f, topicName=细胞癌)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e457184, createdName=qingting, createdTime=Wed Feb 14 18:00:00 CST 2018, time=2018-02-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2005662, encodeId=f26d20056620e, content=<a href='/topic/show?id=6ff1525e13' target=_blank style='color:#2F92EE;'>#CRN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5257, encryptionId=6ff1525e13, topicName=CRN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=35fc2500023, createdName=yanjie6253_56072842, createdTime=Wed Feb 28 11:00:00 CST 2018, time=2018-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260149, encodeId=508426014930, content=谢谢了.学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=30331616752, createdName=txqjm, createdTime=Fri Nov 10 01:45:18 CST 2017, time=2017-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1301880, encodeId=43471301880c6, content=<a href='/topic/show?id=b469109e41d' target=_blank style='color:#2F92EE;'>#lncRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10974, encryptionId=b469109e41d, topicName=lncRNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2317301, createdName=xzw113, createdTime=Sun Nov 05 13:00:00 CST 2017, time=2017-11-05, status=1, ipAttribution=)]
    2017-11-10 txqjm

    谢谢了.学习

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1978598, encodeId=483d19e859808, content=<a href='/topic/show?id=3b90e79787f' target=_blank style='color:#2F92EE;'>#细胞癌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=77, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77978, encryptionId=3b90e79787f, topicName=细胞癌)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e457184, createdName=qingting, createdTime=Wed Feb 14 18:00:00 CST 2018, time=2018-02-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2005662, encodeId=f26d20056620e, content=<a href='/topic/show?id=6ff1525e13' target=_blank style='color:#2F92EE;'>#CRN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=82, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5257, encryptionId=6ff1525e13, topicName=CRN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=35fc2500023, createdName=yanjie6253_56072842, createdTime=Wed Feb 28 11:00:00 CST 2018, time=2018-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260149, encodeId=508426014930, content=谢谢了.学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=30331616752, createdName=txqjm, createdTime=Fri Nov 10 01:45:18 CST 2017, time=2017-11-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1301880, encodeId=43471301880c6, content=<a href='/topic/show?id=b469109e41d' target=_blank style='color:#2F92EE;'>#lncRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10974, encryptionId=b469109e41d, topicName=lncRNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2317301, createdName=xzw113, createdTime=Sun Nov 05 13:00:00 CST 2017, time=2017-11-05, status=1, ipAttribution=)]
    2017-11-05 xzw113

    #lncRNA#

    0

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