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J Periodontol:铒钇铝石榴石激光和超声波洁牙机对涂有二氧化钛的钛表面的龈下菌斑的影响

2017-11-11 MedSci MedSci原创

常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响评估:1)牙菌斑和生物腐蚀氧化钛涂层的去除; 2)表面变化感应;和3)对成骨细胞的剩余生物相容性。

常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响评估:1)牙菌斑和生物腐蚀氧化钛涂层的去除; 2)表面变化感应;和3)对成骨细胞的剩余生物相容性。

方法:龈下菌斑涂层钛表面中度粗糙,用乙醇固定,用超声波洁牙机(金属尖)或Er:YAG激光(20.3或38.2 J / cm2)以非接触方式处理。荧光检测残留菌斑。通过扫描电子显微镜评估圆盘表面形态。通过碘化丙锭/ DNA染色试验和共聚焦显微镜分析,细胞骨架,Ki67,骨桥蛋白和碱性磷酸酶的表达以及矿化结节的形成来测定Saos-2成骨细胞在新的和处理的圆盘上的生存能力,附着,增殖和分化。

结果:两种方法都能有效地清除被处理的表面,用超声波洁牙机使菌斑面积减少到11.7%,Er:YAG激光(38.2 J / cm2)减少到≤0.03%。超声处理的圆盘表现出显着的表面变化,二氧化钛(TiO 2)层不完全去除和少量菌斑聚集体,而Er:YAG激光(38.2J / cm 2)完全去除菌斑和TiO 2层,留下微观凹陷表面。两种处理都保持Saos-2成骨细胞表面良好的生物相容性。空气 - 水冷却保持圆盘温度低于47°C的临界阈值。

结论:本研究表明,使用金属尖的超声洁牙机比用高能量Er:YAG激光去除阳极氧化盘上的菌斑和TiO2层的效率要低,尽管两种方法似乎都能恢复被处理表面的足够的骨电导率

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    2018-01-12 30397613
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    2018-09-13 feather89
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    2018-05-19 wrj0131
  4. 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topicList=[TopicDto(id=92808, encryptionId=b1519280810, topicName=超声波)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ea5135506123, createdName=yyj074, createdTime=Thu Mar 22 16:34:00 CST 2018, time=2018-03-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=261005, encodeId=d867261005dd, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Mon Nov 13 13:47:26 CST 2017, time=2017-11-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1279697, encodeId=830b12e96979f, content=<a href='/topic/show?id=265c66e50ea' target=_blank style='color:#2F92EE;'>#激光#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=137, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66750, encryptionId=265c66e50ea, topicName=激光)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4fd6156, createdName=oliver169, createdTime=Mon Nov 13 13:34:00 CST 2017, time=2017-11-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260629, encodeId=c51e260629d6, content=不错的啊文章值得推荐的, beContent=null, objectType=article, channel=null, level=null, likeNumber=121, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=eff51996281, createdName=1e1b8538m79(暂无匿称), createdTime=Sat Nov 11 23:25:50 CST 2017, time=2017-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260620, encodeId=9a6126062048, content=常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响评估:1)牙菌斑和生物腐蚀氧化钛涂层的去除,2)表面变化感应,和3)对成骨细胞的剩余生物相容性., beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, 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level=null, likeNumber=135, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5K4BGdu4COj6QWFqgMd3xnWDdgLZKUGI32veZvO2qFAQvpYr1eMbUOTqMvlZWc3MsugtFMHzxDwial/0, createdBy=88731939304, createdName=圣艮山, createdTime=Sat Nov 11 22:37:21 CST 2017, time=2017-11-11, status=1, ipAttribution=)]
    2018-03-22 yyj074
  5. 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encryptionId=265c66e50ea, topicName=激光)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4fd6156, createdName=oliver169, createdTime=Mon Nov 13 13:34:00 CST 2017, time=2017-11-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260629, encodeId=c51e260629d6, content=不错的啊文章值得推荐的, beContent=null, objectType=article, channel=null, level=null, likeNumber=121, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=eff51996281, createdName=1e1b8538m79(暂无匿称), createdTime=Sat Nov 11 23:25:50 CST 2017, time=2017-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260620, encodeId=9a6126062048, content=常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响评估:1)牙菌斑和生物腐蚀氧化钛涂层的去除,2)表面变化感应,和3)对成骨细胞的剩余生物相容性., beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, 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level=null, likeNumber=135, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5K4BGdu4COj6QWFqgMd3xnWDdgLZKUGI32veZvO2qFAQvpYr1eMbUOTqMvlZWc3MsugtFMHzxDwial/0, createdBy=88731939304, createdName=圣艮山, createdTime=Sat Nov 11 22:37:21 CST 2017, time=2017-11-11, status=1, ipAttribution=)]
    2017-11-13 yfjms

    学习

    0

  6. 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encryptionId=265c66e50ea, topicName=激光)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4fd6156, createdName=oliver169, createdTime=Mon Nov 13 13:34:00 CST 2017, time=2017-11-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260629, encodeId=c51e260629d6, content=不错的啊文章值得推荐的, beContent=null, objectType=article, channel=null, level=null, likeNumber=121, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=eff51996281, createdName=1e1b8538m79(暂无匿称), createdTime=Sat Nov 11 23:25:50 CST 2017, time=2017-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260620, encodeId=9a6126062048, content=常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响评估:1)牙菌斑和生物腐蚀氧化钛涂层的去除,2)表面变化感应,和3)对成骨细胞的剩余生物相容性., beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7cJ6ejWUr2nIia8qOvAKNdLHr32Df6G8iaoaOx6pHaoiacwp26DaslxZHCGbibZhhCGven4rvmWP60GahtzgFjgCjR/0, createdBy=6b111703011, createdName=有备才能无患, createdTime=Sat Nov 11 23:21:20 CST 2017, time=2017-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260604, encodeId=982426060452, content=很好的文章.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Sat Nov 11 22:48:11 CST 2017, time=2017-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260601, encodeId=6d8d26060165, content=学习了不少事情!!, beContent=null, objectType=article, channel=null, level=null, likeNumber=135, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5K4BGdu4COj6QWFqgMd3xnWDdgLZKUGI32veZvO2qFAQvpYr1eMbUOTqMvlZWc3MsugtFMHzxDwial/0, createdBy=88731939304, createdName=圣艮山, createdTime=Sat Nov 11 22:37:21 CST 2017, time=2017-11-11, status=1, ipAttribution=)]
    2017-11-13 oliver169
  7. 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topicList=[TopicDto(id=92808, encryptionId=b1519280810, topicName=超声波)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ea5135506123, createdName=yyj074, createdTime=Thu Mar 22 16:34:00 CST 2018, time=2018-03-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=261005, encodeId=d867261005dd, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Mon Nov 13 13:47:26 CST 2017, time=2017-11-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1279697, encodeId=830b12e96979f, content=<a href='/topic/show?id=265c66e50ea' target=_blank style='color:#2F92EE;'>#激光#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=137, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66750, encryptionId=265c66e50ea, topicName=激光)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4fd6156, createdName=oliver169, createdTime=Mon Nov 13 13:34:00 CST 2017, time=2017-11-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260629, encodeId=c51e260629d6, content=不错的啊文章值得推荐的, beContent=null, objectType=article, channel=null, level=null, likeNumber=121, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=eff51996281, createdName=1e1b8538m79(暂无匿称), createdTime=Sat Nov 11 23:25:50 CST 2017, time=2017-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=260620, encodeId=9a6126062048, content=常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响评估:1)牙菌斑和生物腐蚀氧化钛涂层的去除,2)表面变化感应,和3)对成骨细胞的剩余生物相容性., beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, 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level=null, likeNumber=135, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5K4BGdu4COj6QWFqgMd3xnWDdgLZKUGI32veZvO2qFAQvpYr1eMbUOTqMvlZWc3MsugtFMHzxDwial/0, createdBy=88731939304, createdName=圣艮山, createdTime=Sat Nov 11 22:37:21 CST 2017, time=2017-11-11, status=1, ipAttribution=)]
    2017-11-11 1e1b8538m79(暂无匿称)

    不错的啊文章值得推荐的

    0

  8. 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level=null, likeNumber=135, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5K4BGdu4COj6QWFqgMd3xnWDdgLZKUGI32veZvO2qFAQvpYr1eMbUOTqMvlZWc3MsugtFMHzxDwial/0, createdBy=88731939304, createdName=圣艮山, createdTime=Sat Nov 11 22:37:21 CST 2017, time=2017-11-11, status=1, ipAttribution=)]
    2017-11-11 有备才能无患

    常规超声洁牙机与铒:钇铝石榴石(Er:YAG)激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响评估:1)牙菌斑和生物腐蚀氧化钛涂层的去除,2)表面变化感应,和3)对成骨细胞的剩余生物相容性.

    0

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level=null, likeNumber=135, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib06iaV3D9Tg5K4BGdu4COj6QWFqgMd3xnWDdgLZKUGI32veZvO2qFAQvpYr1eMbUOTqMvlZWc3MsugtFMHzxDwial/0, createdBy=88731939304, createdName=圣艮山, createdTime=Sat Nov 11 22:37:21 CST 2017, time=2017-11-11, status=1, ipAttribution=)]
    2017-11-11 清风拂面

    很好的文章.谢谢分享

    0

  10. 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    2017-11-11 圣艮山

    学习了不少事情!!

    0

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