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J CLin Periodontol:用喷砂抛光和冷等离子体清除生物膜覆盖的钛片后成骨细胞的生长

2017-06-22 MedSci MedSci原创

目的:研究适宜喷砂抛光与冷等离子体装置结合去除生物膜对体外细胞的影响。

目的:研究适宜喷砂抛光与冷等离子体装置结合去除生物膜对体外细胞的影响。

材料和方法:使用赤藓糖醇粉末(AP)喷砂抛光装置或冷气压氩等离子体(CAP)装置或两者(AP + CAP)结合,去除粗糙的钛片上形成7天的生物膜。随后,通过成骨细胞样细胞(MG-63)的细胞接种来评估去除效力,5天后通过扫描电子显微镜分析孵育细胞扩散程度。另外,通过测量各个处理组钛片的水接触角(WCA)来分析表面亲水性。

结果:AP机械去除样品菌班有利于细胞生长,细胞覆盖表面85%。与AP相比,AP + CAP组合并无优势(细胞覆盖范围57%~75%)。CAP单独处理后,微生物可再生长并破坏所有细胞。三个处理组的WCA均减小。

结论:AP处理有可能完全去除粗糙种植体表面的生物膜。而AP + CAP结合治疗不同于我们的假设,并不能提高成骨细胞的扩散度。

原始出处:

Rutger Matthes, Kathrin Duske, Tewodros Getachew Kebede, et al. Osteoblast growth, after cleaning of biofilm-covered titanium discs with air-polishing and cold plasma. Journal of Clinical Periodontology. 2017 May 16. doi: 10.1111/jcpe.12720.

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    2018-01-11 feather89
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    2017-08-16 30397613
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    2017-06-24 zhaojie88
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