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Diabetologia:使用HLA-A2多聚体组合检测自体反应性CD8 + T细胞

2018-04-12 MedSci MedSci原创

目前,我们需要经过验证的生物标志物来监测1型糖尿病患者的免疫干预效果。尽管这很重要,但对于监测抗原特异性T细胞的选择却很少。先前的报道描述了一些组合方法,它能够同时检测和定量多个胰岛特异性CD8 + T细胞群。近日,国际杂志 《Diabetologia》上在线发表一项由糖尿病协会免疫学会的T细胞多中心研究。在这里,研究人员开展研究以评估多中心环境下组合HLA-A2多聚体检测的性能。 在5个

目前,我们需要经过验证的生物标志物来监测1型糖尿病患者的免疫干预效果。尽管这很重要,但对于监测抗原特异性T细胞的选择却很少。先前的报道描述了一些组合方法,它能够同时检测和定量多个胰岛特异性CD8 + T细胞群。近日,国际杂志 《Diabetologia》上在线发表一项由糖尿病协会免疫学会的T细胞多中心研究。在这里,研究人员开展研究以评估多中心环境下组合HLA-A2多聚体检测的性能。 在5个参与中心,研究人员使用核心试剂和盲样重复样品开展组合HLA-A2多聚体分析。在初步实验中,使用抗原多聚体分析来自健康供体的样品。在随后的实验中,使用β细胞抗原和抗原多聚体分析来自健康供体和1型糖尿病个体的样品。 在每个参与中心成功实施组合分析,重复样品之间的CV表明病毒表位具有良好的重现性(平均%CV = 33.8)。对于β细胞表位,该测定法在单中心设置中非常有效(平均%CV=18.4),但在多中心重复测定中显示出六倍大的变异性(平均%CV = 119)。一般而言,在患有1型糖尿病的个体中比在健康供体中更普遍地检测到β细胞抗原特异性CD8 + T细胞。此外,发现识别HLA-A2限制性胰岛素和谷氨酸脱羧

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    2018-04-14 heli0118
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