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Real-time droplet size analysis using laser micrometer as a process analytical technology tool for continuous dripping process

期刊: ENGINEERING IN LIFE SCIENCES, 2022; 22 (9)

Process analysis and monitoring during the manufacturing of the dripping pills are essential. However, research on developing sensor-based technology ......

JIF:2.463

Conversion of recombinant human ferritin light chain inclusion bodies into uniform nanoparticles in Escherichia coli for facile production

期刊: ENGINEERING IN LIFE SCIENCES, 2022; 22 (6)

Prokaryotic expression systems are widely used to produce many types of biologics because of their extreme conveniences and unmatchable cost. However,......

JIF:2.463

Analysis of microbial diversity and succession during Xiaoqu Baijiu fermentation using high-throughput sequencing technology

期刊: ENGINEERING IN LIFE SCIENCES, 2022; 22 (7)

In this study, high-throughput sequencing (HTS) was used to compare and analyze the microbial diversity and succession during the brewing process of x......

JIF:2.463

Study on dynamic changes of microbial community and lignocellulose transformation mechanism during green waste composting

期刊: ENGINEERING IN LIFE SCIENCES, 2022; 22 (5)

There are few reports on the material transformation and dominant microorganisms in the process of greening waste (GW) composting. In this study, the ......

JIF:2.463

Biosynthesis of (R)-(+)-perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase

期刊: ENGINEERING IN LIFE SCIENCES, 2022; 22 (5)

(R)-(+)-perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)-(+)-perillyl alcohol was investigated......

JIF:2.463

Novel application of anti-human Fc nanobody for screening high-producing CHO cells for monoclonal antibody

期刊: ENGINEERING IN LIFE SCIENCES, 2022; 22 (10)

Animal-derived anti-IgG secondary antibodies are currently employed to stain and screen of human monoclonal antibody(mAb)-producing cells, but using a......

JIF:2.463

Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high-gradient magnetic field

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (6)

Enzymatic degradation of emerging contaminants has gained great interest for the past few years. However, free enzyme often incurs high costs in pract......

Efficient co-production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (6)

To improve the fermentation efficiency of Propionibacterium acidipropionici, a semi-continuous coupled fermentation process was established to achieve......

Yeast autonomously replicating sequence (ARS): Identification, function, and modification

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (7)

Eukaryotic DNA replication begins with multiple origins of replication (ORIs) and thus exploring and identifying ORIs is essential for further underst......

Purification and characterization of the receptor-binding domain of SARS-CoV-2 spike protein from Escherichia coli

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (6)

SARS-CoV-2 is responsible for a disruptive worldwide viral pandemic, and renders a severe respiratory disease known as COVID-19. Spike protein of SARS......

A novel twin-column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (6)

Downstream processing of mAb charge variants is difficult owing to their similar molecular structures and surface charge properties. This study aimed ......

One-step selective affinity purification and immobilization of His-tagged enzyme by recyclable magnetic nanoparticles

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (6)

The NiFe2O4 magnetic nanoparticles (NF-MNPs) were prepared for one-step selective affinity purification and immobilization of His-tagged recombinant g......

A novel downstream process for highly pure 1,3-propanediol from an efficient fed-batch fermentation of raw glycerol by Clostridium pasteurianum

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (6)

An efficient downstream process without prior desalination was developed for recovering 1,3-propanediol (1,3-PDO) with high purity and yield from brot......

Cost-effective purification process development for chimeric hepatitis B core (HBc) virus-like particles assisted by molecular dynamic simulation

期刊: ENGINEERING IN LIFE SCIENCES, 2021; 21 (6)

Inserting foreign epitopes to hepatitis B core (HBc) virus-like particles (VLPs) could influence the molecular conformation and therefore vary the pur......

Preparation of lignosulfonate-based nanofiltration membranes with improved water desalination performance

期刊: ENGINEERING IN LIFE SCIENCES, ; ()

Pulping and papermaking generate large amounts of waste in the form of lignosulfonates which have limited valorized applications so far. Herein, we re......

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