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circFL-seq, A Full-length circRNA Sequencing Method

期刊: BIO-PROTOCOL, 2022; 12 (8)

Due to overlapping sequences with linear cognates, identifying internal sequences of circular RNA (circRNA) remains a challenge. Recently, we have dev......

Fractionation and Extraction of Crude Nuclear Proteins From Arabidopsis Seedlings

期刊: BIO-PROTOCOL, 2022; 12 (2)

The plant nucleus is an important subcellular organelle that contains the genome, ribosomal RNA, and regulatory proteins, and performs a central role ......

Activation of Mitochondrial Ca2+ Oscillation and Mitophagy Induction by Femtosecond Laser Photostimulation

期刊: BIO-PROTOCOL, 2022; 12 (7)

Ultra-precise stimulation solely to individual mitochondria, without any influence to the whole cell, is quite difficult by traditional biochemical re......

14C-paraquat Efflux Assay Arabidopsis Mesophyll Protoplasts in

期刊: BIO-PROTOCOL, 2022; 12 (18)

Weeds compete with crops for growth resources, causing tremendous yield losses. Paraquat is one of the three most common non-selective herbicides. To ......

Measuring Endogenous GA and IAA

期刊: BIO-PROTOCOL, 2022; 12 (4)

Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were a......

Quantitative and Anatomical Imaging of Human Skin by Noninvasive Photoacoustic Dermoscopy

期刊: BIO-PROTOCOL, 2022; 12 (7)

Imaging plays a vital role in the diagnosis and treatment of skin diseases. However, pure optical imaging technique is limited to the visualization of......

A Simple Technique for Direct Immobilization of Target Enzymes from Cell Lysates Based on the SpyTag/SpyCatcher Spontaneous Reaction

期刊: BIO-PROTOCOL, 2022; 12 (1)

Many of the current methods for enzyme purification and immobilization suffer from several drawbacks, such as requiring tedious multistep procedures o......

Expression and Purification of the Human Cation-chloride Cotransporter KCC1 from HEK293F Cells for Structural Studies

期刊: BIO-PROTOCOL, 2021; 11 (7)

Cation-chloride cotransporters (CCCs) mediate the coupled, electroneutral symport of cations such as Na+ and/or K+ with chloride across membrane. Amon......

Visualization of Host Cell Kinase Activation by Viral Proteins Using GFP Fluorescence Complementation and Immunofluorescence Microscopy

期刊: BIO-PROTOCOL, 2021; 11 (13)

Non-receptor protein-tyrosine kinases regulate cellular responses to many external signals and are important drug discovery targets for cancer and inf......

A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation

期刊: BIO-PROTOCOL, 2021; 11 (15)

Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeuti......

Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta

期刊: BIO-PROTOCOL, 2021; 11 (16)

Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. sola......

Investigate Synaptic Vesicles Mobility in Neuronal Culture Axons by FRAP Imaging

期刊: BIO-PROTOCOL, 2021; 11 (6)

Synaptic vesicles (SVs) are clustered in the presynaptic terminals and consistently trafficking along axons. Based on their release features, SVs are ......

Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS

期刊: BIO-PROTOCOL, 2021; 11 (13)

Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues p......

In vitro Reconstitution Assays of Arabidopsis 20S Proteasome

期刊: BIO-PROTOCOL, 2021; 11 (7)

The majority of cellular proteins are degraded by the 26S proteasome in eukaryotes. However, intrinsically disordered proteins (IDPs), which contain l......

A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves

期刊: BIO-PROTOCOL, 2021; 11 (15)

Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure......

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