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A Simple Technique for Direct Immobilization of Target Enzymes from Cell Lysates Based on the SpyTag/SpyCatcher Spontaneous Reaction

Cai, LX; Lin, YQ; Chu, YM; Chen, XP; Liu, LX; Zhang, M; Zhang, GY

Zhang, GY (通讯作者),Huaqiao Univ, Dept Bioengn & Biotechnol, Xiamen, Peoples R China.

BIO-PROTOCOL, 2022; 12 (1):

Abstract

Many of the current methods for enzyme purification and immobilization suffer from several drawbacks, such as requiring tedious multistep procedures o......

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