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VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography

Zhang, JG; Zhang, DY; Sun, L; Ji, G; Huang, XJ; Niu, TX; Xu, JS; Ma, CY; Zhu, Y; Gao, N; Xu, W; Sun, F

Sun, F (corresponding author), Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China.

JOURNAL OF STRUCTURAL BIOLOGY, 2021; 213 (3):

Abstract

Cryo-electron tomography (cryo-ET) provides a promising approach to study intact structures of macromolecules in situ, but the efficient preparation o......

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