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VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2021; 213 (3)

Cryo-electron tomography (cryo-ET) provides a promising approach to study intact structures of macromolecules in situ, but the efficient preparation o......

The structure of exopolyphosphatase (PPX) from Porphyromonas gingivalis in complex with substrate analogs and magnesium ions reveals the basis for polyphosphate processivity

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2021; 213 (3)

The enzymes exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GppA) play important roles in the bacterial stringent response. PPX degr......

Structural and biochemical insights into the substrate-binding mechanism of a glycoside hydrolase family 12 beta-1,3-1,4-glucanase from Chaetomium sp.

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2021; 213 (3)

beta-1,3-1,4-Glucanases are a type of hydrolytic enzymes capable of catalyzing the strict cleavage of beta-1,4 glycosidic bonds adjacent to beta-1,3 l......

Crystal structure of TbAlba1 from Trypanosoma brucei

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2021; 213 (3)

Alba (Acetylation lowers binding affinity) domain is a small, dimeric nucleic acid-binding domain, which is widely distributed in archaea and numbers ......

Direct transfer of electron microscopy samples to wetted carbon and graphene films via a support floatation block

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2021; 213 (1)

Support films are commonly used during cryo-EM specimen preparation to both immobilise the sample and minimise the exposure of particles at the air-wa......

Molecular basis for cysteine oxidation by plant cysteine oxidases from Arabidopsis thaliana

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2021; 213 (1)

Plant Cysteine Oxidases (PCOs) play important roles in controlling the stability of Group VII ethylene response factors (ERF-VIIs) via Arg/N-degron pa......

Nanostructure of mouse otoconia

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 210 (2)

Mammalian otoconia of the inner ear vestibular apparatus are calcium carbonate-containing mineralized structures critical for maintaining balance and ......

Structure of the enterovirus D68 RNA-dependent RNA polymerase in complex with NADPH implicates an inhibitor binding site in the RNA template tunnel

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 211 (1)

Enterovirus D68 (EV-D68) is an emerging viral pathogen belonging to the Enterovirus genus of the Picornaviridae family, which is a serious threat to h......

Structural characterization of a bat Adeno-associated virus capsid

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 211 (2)

Adeno-associated viruses (AAVs) are widespread among vertebrates. AAVs isolated from bats display low capsid protein sequence identities (< 60%) to......

Exaptation of two ancient immune proteins into a new dimeric pore-forming toxin in snails

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 211 (2)

The Membrane Attack Complex-Perforin (MACPF) family is ubiquitously found in all kingdoms. They have diverse cellular roles, however MACPFs with pore-......

Unit quaternion description of spatial rotations in 3D electron cryo-microscopy

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 212 (3)

Electron cryo-microscopy (cryoEM) involves the estimation of spatial rotations, or saying orientations, of projection images or three-dimensional (3D)......

An optimized approach using cryofixation for high-resolution 3D analysis by FIB-SEM

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 212 (1)

Compared with conventional two-dimensional transmission electron microscopy (TEM), focused ion beam scanning electron microscopy (FIB-SEM) can provide......

The variable oligomeric state of Amuc_1100 from Akkermansia muciniphila

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 212 (1)

Akkermansia muciniphila is a beneficial microorganism colonized in the human gut that can reverse many intestinal metabolic-related diseases. Amuc_110......

Multiscale structural evolution of citrate-triggered intrafibrillar and interfibrillar mineralization in dense collagen gels

期刊: JOURNAL OF STRUCTURAL BIOLOGY, 2020; 212 (1)

The mineralized extracellular matrix of bone is an organic-inorganic nanocomposite consisting primarily of carbonated hydroxyapatite, fibrous type I c......

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